bicoid mRNA localises to the Drosophila oocyte anterior by random Dynein-mediated transport and anchoring.

Abstract:

:bicoid mRNA localises to the Drosophila oocyte anterior from stage 9 of oogenesis onwards to provide a local source for Bicoid protein for embryonic patterning. Live imaging at stage 9 reveals that bicoid mRNA particles undergo rapid Dynein-dependent movements near the oocyte anterior, but with no directional bias. Furthermore, bicoid mRNA localises normally in shot2A2, which abolishes the polarised microtubule organisation. FRAP and photo-conversion experiments demonstrate that the RNA is stably anchored at the anterior, independently of microtubules. Thus, bicoid mRNA is localised by random active transport and anterior anchoring. Super-resolution imaging reveals that bicoid mRNA forms 110-120 nm particles with variable RNA content, but constant size. These particles appear to be well-defined structures that package the RNA for transport and anchoring.

journal_name

Elife

journal_title

eLife

authors

Trovisco V,Belaya K,Nashchekin D,Irion U,Sirinakis G,Butler R,Lee JJ,Gavis ER,St Johnston D

doi

10.7554/eLife.17537

subject

Has Abstract

pub_date

2016-10-28 00:00:00

issn

2050-084X

journal_volume

5

pub_type

杂志文章

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