Abstract:
:bicoid mRNA localises to the Drosophila oocyte anterior from stage 9 of oogenesis onwards to provide a local source for Bicoid protein for embryonic patterning. Live imaging at stage 9 reveals that bicoid mRNA particles undergo rapid Dynein-dependent movements near the oocyte anterior, but with no directional bias. Furthermore, bicoid mRNA localises normally in shot2A2, which abolishes the polarised microtubule organisation. FRAP and photo-conversion experiments demonstrate that the RNA is stably anchored at the anterior, independently of microtubules. Thus, bicoid mRNA is localised by random active transport and anterior anchoring. Super-resolution imaging reveals that bicoid mRNA forms 110-120 nm particles with variable RNA content, but constant size. These particles appear to be well-defined structures that package the RNA for transport and anchoring.
journal_name
Elifejournal_title
eLifeauthors
Trovisco V,Belaya K,Nashchekin D,Irion U,Sirinakis G,Butler R,Lee JJ,Gavis ER,St Johnston Ddoi
10.7554/eLife.17537subject
Has Abstractpub_date
2016-10-28 00:00:00issn
2050-084Xjournal_volume
5pub_type
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