Abstract：
:A peptide of unknown structure was found as a side product in a commercial preparation of the 1-34 fragment of bovine parathyroid hormone (PTH). CNBr cleavage and amino acid analysis showed that this peptide is the des-lys-13 form of 1-34 bovine PTH. The peptide thus represents a deletion mutant of PTH and structure-function studies are of interest. This peptide was a full agonist in the adenylyl cyclase bioassay for PTH, but its potency was about 5% of that found for the complete 1-34 peptide. Proton NMR studies showed that the pK values for the histidine residues in the des-lys-13 form were essentially identical to those of the intact peptide. However, pH-dependent changes in the chemical shifts for the tryptophan protons (residue 23) and several unidentified methyl group resonances were observed in the des-lys peptide. The latter are major shifts and probably represent ring-current effects; these were not seen in the intact 1-34 peptide. The results show that Lys-13 is important in the folding of the active domain of PTH, and are interpreted in the context of a previously published model for the folding of this hormone.
journal_name
Mol Cell Endocrinoljournal_title
Molecular and cellular endocrinologyauthors
Zull JE,Smith LM,Chuang J,Jentoft Jdoi
10.1016/0303-7207(87)90037-2subject
Has Abstractpub_date
1987-06-01 00:00:00pages
267-71issue
3eissn
0303-7207issn
1872-8057journal_volume
51pub_type
杂志文章abstract：:NPS(o-nitrophenyl sulfenyl)-ACTH stimulated steroidogenesis in Y1 cells to the same maximum level as did ACTH, but with a 60-fold lower potency than the native hormone. NPS-ACTH also stimulated the extracellular accumulation of cAMP in Y1 cells with lower potency than the unmodified hormone. The amount of cAMP accumul...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(80)90111-2
更新日期：1980-03-01 00:00:00
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journal_title：Molecular and cellular endocrinology
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更新日期：2011-03-30 00:00:00
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journal_title：Molecular and cellular endocrinology
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更新日期：2014-02-15 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
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更新日期：2011-01-15 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
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更新日期：2003-03-28 00:00:00
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journal_title：Molecular and cellular endocrinology
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更新日期：2006-03-27 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
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更新日期：1994-05-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2019.110636
更新日期：2020-01-15 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(95)03715-2
更新日期：1996-02-05 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2019.03.001
更新日期：2019-04-15 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2015.09.031
更新日期：2015-12-05 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2020.111098
更新日期：2021-02-05 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.mce.2013.05.012
更新日期：2013-11-05 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(76)90007-1
更新日期：1975-12-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2014.10.025
更新日期：2015-02-05 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(91)90269-x
更新日期：1991-04-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/s0303-7207(01)00588-3
更新日期：2001-10-25 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(93)90231-8
更新日期：1993-12-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
doi：10.1016/0303-7207(90)90244-3
更新日期：1990-06-18 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.mce.2012.12.024
更新日期：2013-05-22 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2005.10.008
更新日期：2005-12-21 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2015.09.025
更新日期：2015-12-05 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2017.01.051
更新日期：2017-03-15 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2016.06.028
更新日期：2016-10-15 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.mce.2016.08.047
更新日期：2017-11-05 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(92)90104-e
更新日期：1992-12-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(82)90017-x
更新日期：1982-04-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
doi：10.1016/s0303-7207(96)03952-4
更新日期：1996-12-20 00:00:00
abstract：:Evidence from in vitro studies and naturally occurring human diseases indicate that several endocrine cells, and particularly somatotrophs, recognize cAMP as a growth factor. Accordingly, mutations of the alpha subunit of the stimulatory G protein gene (GNAS) leading to the constitutive activation of adenylyl cyclase ...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.mce.2010.04.009
更新日期：2010-09-15 00:00:00
abstract：:Fibroblast growth factor (FGF)-21 is an endocrine member of the FGF family with healthy effects on glucose and lipid metabolism. FGF21 reduces glycemia and lipidemia in rodent models of obesity and type 2 diabetes. In addition to its effects improving insulin sensitivity, FGF21 causes weight loss by increasing energy ...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.mce.2015.09.018
更新日期：2015-12-15 00:00:00
