Abstract：
:The translocator protein 18 kDa (TSPO), initially characterized as peripheral benzodiazepine receptor, is a conserved outer mitochondrial membrane protein, implicated in cholesterol transport thereby affecting steroid hormone biosynthesis, as well as in general mitochondrial function related to bioenergetics, oxidative stress, and Ca2+ homeostasis. TSPO is highly expressed in steroidogenic tissues such as adrenal glands, but shows low expression in the central nervous system. During various disease states such as inflammation, neurodegeneration or cancer, the expression of mitochondrial TSPO in affected tissues is upregulated. The expression of TSPO can be traced for diagnostic purpose by high affinity radio-ligands. Moreover, the function of TSPO is modulated by synthetic as well as endogenous ligands with agonistic or antagonistic properties. Thus, TSPO ligands serve functions as both important biomarkers and putative therapeutic agents. In the present study, we aimed to characterize the effects of TSPO ligands on mouse BV-2 microglia cells, which express significant levels of TSPO, and analyzed the effect of XBD173, PK11195, and Ro5-4864, as well as the inflammatory reagent Lipopolysaccharides (LPS) on neurosteroid synthesis and on basic mitochondrial functions such as oxidative phosphorylation, mitochondrial membrane potential and Ca2+ homeostasis. Specific TSPO-dependent effects were separated from off-target effects by comparing lentiviral TSPO knockdown with shRNA scramble-controls and wild-type BV-2 cells. Our data demonstrate ligand-specific effects on different cellular functions in a TSPO-dependent or independent manner, providing evidence for both specific TSPO-mediated, as well as off-target effects.
journal_name
Psychoneuroendocrinologyjournal_title
Psychoneuroendocrinologyauthors
Bader S,Wolf L,Milenkovic VM,Gruber M,Nothdurfter C,Rupprecht R,Wetzel CHdoi
10.1016/j.psyneuen.2019.03.029subject
Has Abstractpub_date
2019-08-01 00:00:00pages
65-76eissn
0306-4530issn
1873-3360pii
S0306-4530(18)30944-2journal_volume
106pub_type
杂志文章abstract：BACKGROUND:It has been suggested that depressed persons have increased oxidative stress and decreased anti-oxidant defences. 8-Hydroxy-2'-deoxyguanosine (8-OHdG) and F2-isoprostanes, measures of oxidative DNA and lipid damage respectively, are among the most reliable oxidative stress markers, but studies on their assoc...
journal_title：Psychoneuroendocrinology
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.psyneuen.2014.09.025
更新日期：2015-01-01 00:00:00
abstract：:The effects of corticosteroids on memory performance have been the subject of some controversy. Whilst many studies have shown that high levels of corticosteroids can impair memory performance, others have shown they can facilitate it. One explanation for these discrepant effects arises from the differential activatio...
journal_title：Psychoneuroendocrinology
pub_type： 临床试验,杂志文章
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更新日期：2004-07-01 00:00:00
abstract：:Adolescent animals are vulnerable to the effects of stress on brain development. We hypothesized that long-term effects of adolescent chronic stress are mediated by glucocorticoid receptor (GR) signaling. We used a specific GR modulator (CORT108297) to pharmacologically disrupt GR signaling in adolescent rats during e...
journal_title：Psychoneuroendocrinology
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更新日期：2020-02-01 00:00:00
abstract：BACKGROUND:Alterations of hypothalamic-pituitary-adrenal (HPA) axis function and sympathetic-adrenal activity have been proposed as key factors in biological models of posttraumatic stress disorder (PTSD). METHODS:We examined neuroendocrine function in female survivors of intimate partner violence (IPV) with lifetime ...
journal_title：Psychoneuroendocrinology
pub_type： 杂志文章
doi：10.1016/j.psyneuen.2006.03.007
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journal_title：Psychoneuroendocrinology
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journal_title：Psychoneuroendocrinology
pub_type： 临床试验,杂志文章
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abstract：:Determinations of gonadotropins and testosterone were performed in 22 men with hypospadias (H), seven male-to-female transsexuals (T) and in 16 normal men (N), all of similar age. The response of serum FSH and LH to GnRH was determined in six subjects in each group. Oligospermia was found in five of 13 patients with H...
journal_title：Psychoneuroendocrinology
pub_type： 杂志文章
doi：10.1016/0306-4530(86)90032-6
更新日期：1986-01-01 00:00:00
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journal_title：Psychoneuroendocrinology
pub_type： 临床试验,杂志文章,随机对照试验
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journal_title：Psychoneuroendocrinology
pub_type： 临床试验,杂志文章
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更新日期：2001-04-01 00:00:00
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journal_title：Psychoneuroendocrinology
pub_type： 杂志文章,随机对照试验
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journal_title：Psychoneuroendocrinology
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.psyneuen.2010.09.009
更新日期：2011-06-01 00:00:00
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doi：10.1016/j.psyneuen.2017.10.005
更新日期：2018-01-01 00:00:00
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journal_title：Psychoneuroendocrinology
pub_type： 临床试验,杂志文章,随机对照试验
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journal_title：Psychoneuroendocrinology
pub_type： 临床试验,杂志文章,随机对照试验
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journal_title：Psychoneuroendocrinology
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doi：10.1016/j.psyneuen.2017.12.023
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journal_title：Psychoneuroendocrinology
pub_type： 临床试验,杂志文章,随机对照试验
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pub_type： 杂志文章
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journal_title：Psychoneuroendocrinology
pub_type： 杂志文章
doi：10.1016/j.psyneuen.2020.105095
更新日期：2020-12-01 00:00:00
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journal_title：Psychoneuroendocrinology
pub_type： 临床试验,杂志文章
doi：10.1016/j.psyneuen.2013.11.010
更新日期：2014-02-01 00:00:00
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journal_title：Psychoneuroendocrinology
pub_type： 杂志文章,随机对照试验
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更新日期：2017-03-01 00:00:00
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journal_title：Psychoneuroendocrinology
pub_type： 杂志文章
doi：10.1016/j.psyneuen.2018.08.016
更新日期：2018-12-01 00:00:00
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journal_title：Psychoneuroendocrinology
pub_type： 杂志文章
doi：10.1016/j.psyneuen.2014.12.004
更新日期：2015-02-01 00:00:00
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更新日期：2019-11-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0306-4530(92)90018-3
更新日期：1992-11-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/s0306-4530(02)00026-4
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pub_type： 杂志文章
doi：10.1016/j.psyneuen.2021.105150
更新日期：2021-01-20 00:00:00
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journal_title：Psychoneuroendocrinology
pub_type： 杂志文章
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更新日期：2016-01-01 00:00:00
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journal_title：Psychoneuroendocrinology
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更新日期：2006-10-01 00:00:00
