Abstract：
:Non-viral gene delivery vectors studied in the gene therapy applications are often designed with the cationic nitrogen containing groups necessary for binding and cell release of nucleic acids. Disadvantage is a relatively high toxicity which restricts the in vivo use of such nanoparticles. Here we show, that the 3rd generation carbosilane dendrimers possessing (trimethyl)phosphonium (PMe3) groups on their periphery were able to effectively deliver the functional siRNA into the cells (B14, Cricetulus griseus), release it into the cytosol and finally to achieve up to 40% gene silencing of targeted gene (glyceraldehyde-3-phosphate dehydrogenase (GAPDH)) with the comparable or, in some cases, even better effectivity as their ammonium counterparts. Moreover, such cationic dendrimers show relatively low in vivo toxicity as compared to their ammonium analogues when analyzed by standard fish embryo test (FET) on Danio rerio in vivo model, with LD50 = 6.26 µM after 48 h of incubation. This is more than 10-fold improvement as compared to published values for various other types of cationic dendrimers. We discuss the potential of further increase of the transfection efficiency, endosomal escape and decrease of toxicity of such non-viral vectors, based on the systematic screening of different types of substituents on central phosphonium atom.
journal_name
Int J Pharmjournal_title
International journal of pharmaceuticsauthors
Herma R,Wrobel D,Liegertová M,Müllerová M,Strašák T,Maly M,Semerádtová A,Štofik M,Appelhans D,Maly Jdoi
10.1016/j.ijpharm.2019.03.018subject
Has Abstractpub_date
2019-05-01 00:00:00pages
51-65eissn
0378-5173issn
1873-3476pii
S0378-5173(19)30196-6journal_volume
562pub_type
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