Abstract:
:The genetics underlying autism spectrum disorder (ASD) are complex. Approximately 3-5% of ASD cases arise from maternally inherited duplications of 15q11.2-q13.1, termed Duplication 15q syndrome (Dup15q). 15q11.2-q13.1 includes the gene UBE3A which is believed to underlie ASD observed in Dup15q syndrome. UBE3A is an E3 ubiquitin ligase that targets proteins for degradation and trafficking, so finding UBE3A substrates and interacting partners is critical to understanding Dup15q ASD. In this study, we take an unbiased genetics approach to identify genes that genetically interact with Dube3a, the Drosophila melanogaster homolog of UBE3A. We conducted an enhancer/suppressor screen using a rough eye phenotype produced by Dube3a overexpression with GMR-GAL4. Using the DrosDel deficiency kit, we identified 3 out of 346 deficiency lines that enhanced rough eyes when crossed to two separate Dube3a overexpression lines, and subsequently identified IA2, GABA-B-R3, and lola as single genes responsible for rough eye enhancement. Using the FlyLight GAL4 lines to express uas-Dube3a + uas-GFP in the endogenous lola pattern, we observed an increase in the GFP signal compared to uas-GFP alone, suggesting a transcriptional co-activation effect of Dube3a on the lola promoter region. These findings extend the role of Dube3a/UBE3A as a transcriptional co-activator, and reveal new Dube3a interacting genes.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Hope KA,McGinn A,Reiter LTdoi
10.1038/s41598-019-38663-ysubject
Has Abstractpub_date
2019-02-20 00:00:00pages
2382issue
1issn
2045-2322pii
10.1038/s41598-019-38663-yjournal_volume
9pub_type
杂志文章abstract::Repetitive transcranial magnetic stimulation (rTMS) has been proposed as a tinnitus treatment option. Promising results have been obtained by consecutive stimulation of lateral frontal and auditory brain regions. We investigated a combined stimulation paradigm targeting the anterior cingulate cortex (ACC) with double ...
journal_title:Scientific reports
pub_type: 杂志文章,随机对照试验
doi:10.1038/srep18028
更新日期:2015-12-15 00:00:00
abstract::Although increases in cardiovascular load (pressure overload) are known to elicit ventricular remodeling including cardiomyocyte hypertrophy and interstitial fibrosis, the molecular mechanisms of pressure overload or AngII -induced cardiac interstitial fibrosis remain elusive. In this study, serpinE2/protease nexin-1 ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep37635
更新日期:2016-11-23 00:00:00
abstract::To identify the prevalence and characteristics of community-acquired Clostridium difficile infection (CA-CDI) in southwest China, we conducted a cross-sectional study. 978 diarrhea patients were enrolled and stool specimens' DNA was screened for virulence genes. Bacterial culture was performed and isolates were charac...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-21762-7
更新日期:2018-03-05 00:00:00
abstract::ES-62 is a secreted parasitic worm-derived immunomodulator that exhibits therapeutic potential in allergy by downregulating aberrant MyD88 signalling to normalise the inflammatory phenotype and mast cell responses. IL-33 plays an important role in driving mast cell responses and promoting type-2 allergic inflammation,...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-22716-9
更新日期:2018-03-14 00:00:00
abstract::It is of paramount importance to improve the control over large area growth of high quality molybdenum disulfide (MoS2) and other types of 2D dichalcogenides. Such atomically thin materials have great potential for use in electronics, and are thought to make possible the first real applications of spintronics. Here in...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-06699-7
更新日期:2017-07-25 00:00:00
abstract::Strategic delivery of IRDye 700DX (photosensitizer) is a key for improving its effect in photodynamic therapy. In this study, we have synthesized IRDye 700DX-conjugated polymers containing multiple cyclic RGD peptides to deliver IRDye 700DX selectively to tumor cells and tumor-associated blood vessels overexpressing α...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-26593-0
更新日期:2018-05-25 00:00:00
abstract::High frequency repetitive transcranial magnetic stimulation (HF-rTMS) over the left dorsolateral prefrontal cortex (L-DLPFC) is a widely applied treatment protocol for chronic smoking and major depressive disorder. However, no previous study has measured the effects of rTMS on both nicotine consumption and anxiety/dep...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-80927-5
更新日期:2021-01-15 00:00:00
abstract::We propose a method to accelerate small-angle scattering experiments by exploiting spatial correlation in two-dimensional data. We applied kernel density estimation to the average of a hundred short scans and evaluated noise reduction effects of kernel density estimation (smoothing). Although there is no advantage of ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-37345-5
更新日期:2019-02-06 00:00:00
abstract::Human umbilical cord mesenchymal stem cells (huMSCs) can treat primary ovarian insufficiency (POI) related to ovarian granulosa cell (OGC) apoptosis caused by cisplatin chemotherapy. Exosomes are a class of membranous vesicles with diameters of 30-200 nm that are constitutively released by eukaryotic cells. Exosomes m...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-02786-x
更新日期:2017-05-31 00:00:00
abstract::The development of microstructure during melting, reactive wetting and solidification of solder pastes on Cu-plated printed circuit boards has been studied by synchrotron radiography. Using Sn-3.0Ag-0.5Cu/Cu and Sn-0.7Cu/Cu as examples, we show that the interfacial Cu6Sn5 layer is present within 0.05 s of wetting, and...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep40010
更新日期:2017-01-12 00:00:00
abstract::The beetle Homalinotus depressus (Coleoptera: Curculionidae) is a major pest of coconuts in the Northern region of Brazil, for which environmentally friendly methods of control are desired. Behavioral responses of H. depressus to airborne volatile extracts from conspecifics suggested the presence of a male-produced ag...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-37156-8
更新日期:2019-01-28 00:00:00
abstract::To explore soil organic carbon (SOC) accumulation mechanisms, the dynamics of C functional groups and macroaggregation were studied synchronously through aggregate fractionation and (13)C NMR spectroscopy in sandy loam soil following an 18-year application of compost and fertilizer in China. Compared with no fertilize...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep13804
更新日期:2015-09-11 00:00:00
abstract::This study aimed to define the width and length of the dental arch in 12-year-old Vietnamese children, and to elucidate differences between genders and among ethnic groups. A cross-sectional study was conducted in 4565 12 years-old children from the 4 major ethnic groups in Vietnam (Kinh, Muong, Thai, and Tay), with a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-39710-4
更新日期:2019-02-28 00:00:00
abstract::Fowl cholera is a serious, highly contagious disease caused by the bacterium Pasteurella multocida (P. multocida) in a range of avian species and is characterized by an acute form of septicaemia. The pathogenic mechanism of chicken lung injury caused by the bacterium is unclear. Therefore, P. multocida Q (a reference ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-62042-7
更新日期:2020-04-14 00:00:00
abstract::Monocytes and macrophages are key players in maintaining immune homeostasis. Identifying strategies to manipulate their functions via gene delivery is thus of great interest for immunological research and biomedical applications. We set out to establish conditions for mRNA transfection in hard-to-transfect primary hum...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-60506-4
更新日期:2020-03-06 00:00:00
abstract::The Fukushima Daiichi nuclear power reactor units that generated large amounts of airborne discharges during the period of March 12-21, 2011 were identified individually by analyzing the combination of measured (134)Cs/(137)Cs depositions on ground surfaces and atmospheric transport and deposition simulations. Because...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep31376
更新日期:2016-08-22 00:00:00
abstract::The gist of natural scenes can be extracted very rapidly and even without focal attention. However, it is unclear whether and to what extent the gist of natural scenes can break through the bottleneck of crowding, a phenomenon in which object recognition will be immensely impaired. In the first two experiments, a targ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-32455-6
更新日期:2018-09-19 00:00:00
abstract::Stabilization exercise (SE) is evident for the management of chronic non-specific low back pain (LBP). The optimal dose-response-relationship for the utmost treatment success is, thus, still unknown. The purpose is to systematically review the dose-response-relationship of stabilisation exercises on pain and disabilit...
journal_title:Scientific reports
pub_type: 杂志文章,meta分析
doi:10.1038/s41598-020-73954-9
更新日期:2020-10-09 00:00:00
abstract::As a nutritional factor, folic acid can prevent cardiac and neural defects during embryo development. Our previous study showed that arsenic impairs embryo development by down-regulating Dvr1/GDF1 expression in zebrafish. Here, we investigated whether folic acid could protect against arsenic-mediated embryo toxicity. ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep16093
更新日期:2015-11-05 00:00:00
abstract::Coherent Anti-Stokes Raman Spectroscopy (CARS) is performed on single spores (conidia) of the fungus Aspergillus nidulans in order to establish a baseline measurement for fungal spores. Chemical maps of single spores are generated and spectral differentiation between the cell wall and the cytoplasm is achieved. Princi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-37978-6
更新日期:2019-02-11 00:00:00
abstract::Caterpillars of the silk moth genus Hyalophora (Lepidoptera; Saturniidae) construct multilayered cocoons that function as overwintering housing during the pupal to adult developmental period. While all cocoons share the primary function of protecting the developing moth, cocoons spun by different Hyalophora silk moth ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-62547-1
更新日期:2020-03-27 00:00:00
abstract::Cisplatin (CDDP) is potent anticancer agent used for several tumor types. Thymoquinone (TQ) is naturally occurring compound drawing great attention as anticancer and chemomodulator for chemotherapies. Herein, we studied the potential cytotoxicity of thymoquinone, CDDP and their combination against human oral squamous ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-13357-5
更新日期:2017-10-13 00:00:00
abstract::This work provides new insights into the dynamics of silica nanoparticle-based removal of organic fluids (here oil) from naturally occurring porous media. We have used 4D (time-resolved 3D) imaging at pore-scale using X-ray computed micro-tomography (μCT) technique. The captured 3D tomographic time-series data reveal ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-29569-2
更新日期:2018-07-24 00:00:00
abstract::This corrects the article DOI: 10.1038/srep45235. ...
journal_title:Scientific reports
pub_type: 杂志文章,已发布勘误
doi:10.1038/srep46913
更新日期:2017-12-22 00:00:00
abstract::The integral membrane-bound Nrf1 transcription factor fulfils important functions in maintaining cellular homeostasis and organ integrity, but how it is controlled vectorially is unknown. Herein, creative use of Gal4-based reporter assays with protease protection assays (GRAPPA), and double fluorescence protease prote...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep02006
更新日期:2013-01-01 00:00:00
abstract::A combination of a solution process and the control of the electric potential for magnetism represents a new approach to operating spintronic devices with a highly controlled efficiency and lower power consumption with reduced production cost. As a paradigmatic example, we investigated Co/Pt(111) in the Bloch-wall reg...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep43700
更新日期:2017-03-03 00:00:00
abstract::Vibrio vulnificus causes fatal infections in humans, and antibiotics are commonly used in treatment regimens against V. vulnificus infection. However, the therapeutic effects of antibiotics are limited by multidrug resistance. In this study, we demonstrated that an antimicrobial peptide (AMP), HPA3PHis, loaded onto a ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-14127-z
更新日期:2017-10-19 00:00:00
abstract::Organotypic, three-dimensional (3D) cancer models have enabled investigations of complex microtissues in increasingly realistic conditions. However, a drawback of these advanced models remains the poor biological relevance of cancer cell lines, while higher clinical significance would be obtainable with patient-derive...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-06544-x
更新日期:2017-07-26 00:00:00
abstract::We present a formulation of electromagnetic spin-orbit coupling in magneto-optic media, and propose an alternative source of spin-orbit coupling to non-paraxial optics vortices. Our treatment puts forth a formulation of nonreciprocal transverse-spin angular-momentum-density shifts for evanescent waves in magneto-optic...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep39972
更新日期:2017-01-06 00:00:00
abstract::Ecdysteroids (arthropod molting hormones) play an important role in the development and sexual maturation of arthropods, and they have been shown to have anabolic and "energizing" effect in higher vertebrates. The aim of this study was to assess ecdysteroid diversity, levels according to bird species and months, as we...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-53090-9
更新日期:2019-11-18 00:00:00