Abstract:
:ABT-737, ABT-263 (Navitoclax) and ABT-199 (Venetoclax) are under intensive preclinical and clinical investigation as treatments for hematologic and other malignancies. These small molecules mimic pro-death B-cell lymphoma-2 (Bcl-2) Homology 3 (BH3) domain-only proteins. They also bear a structural resemblance to certain sigma (σ) receptor ligands. Moreover, the Bcl-2 and σ receptor protein families are both located primarily at the endoplasmic reticulum, mediate cell death and survival through protein-protein interactions, and physically associate. Accordingly, we examined the ability of the ABT series of BH3 mimetics to interact with σ receptors using radioligand-binding techniques. Negative allosteric modulation of [3H](+)-pentazocine, an agonist, binding to σ1 receptors in guinea pig brain membranes was observed for ABT-737, ABT-263 and ABT-199. Findings included reduction of specific binding to distinct plateaus in concentration-dependent fashion, significant slowing of radioligand dissociation kinetics, and decreases in radioligand affinity with no or modest changes in maximal receptor densities. Using a ternary complex model, dissociation constants (KX) for modulator binding to the σ1 receptor ranged from 1 to 2.5 μM, while negative cooperativity factors (α), representing the changes in affinity of ligand and modulator when bound as a ternary complex with the receptor, ranged from 0.15 to 0.42. These observations were extended and reinforced by studies using intact small cell (NCI-H69) and non-small cell (NCI-H23) lung cancer cells, and by using an antagonist σ1 receptor radioligand, E-N-1-(3'-[125I]iodoallyl)-N'-4-(3″,4″-dimethoxyphenethyl)piperazine, in mouse brain membranes. By contrast, exploratory studies indicate marked enhancement of the σ2 receptor binding of [3H]1,3-di-(o-tolyl)guanidine/(+)-pentazocine in NCI-H23 cells and guinea pig brain membranes. These findings raise intriguing questions regarding mechanism and potential functional outcomes.
journal_name
Pharmacol Resjournal_title
Pharmacological researchauthors
Lever JR,Fergason-Cantrell EAdoi
10.1016/j.phrs.2019.01.040subject
Has Abstractpub_date
2019-04-01 00:00:00pages
87-100eissn
1043-6618issn
1096-1186pii
S1043-6618(18)31579-2journal_volume
142pub_type
杂志文章abstract::Idiopathic pulmonary fibrosis (IPF) is a debilitating condition where excess collagen deposition occurs in the extracellular matrix. At first sight, it is expected that the level of different kinds of matrix metalloproteinases might be downregulated in IPF as it is a matrix degrading collagenase. However, the role of ...
journal_title:Pharmacological research
pub_type: 杂志文章,评审
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journal_title:Pharmacological research
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journal_title:Pharmacological research
pub_type: 临床试验,杂志文章
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
doi:10.1006/phrs.2000.0683
更新日期:2000-09-01 00:00:00
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journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/j.phrs.2011.04.006
更新日期:2011-09-01 00:00:00
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doi:10.1006/phrs.2000.0702
更新日期:2000-10-01 00:00:00
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doi:10.1016/j.phrs.2009.03.013
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journal_title:Pharmacological research
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doi:10.1016/j.phrs.2017.02.003
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pub_type: 杂志文章
doi:10.1016/j.phrs.2019.104512
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journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/s1043-6618(03)00193-2
更新日期:2003-11-01 00:00:00
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journal_title:Pharmacological research
pub_type: 杂志文章,评审
doi:10.1016/j.phrs.2004.05.005
更新日期:2005-01-01 00:00:00
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journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/j.phrs.2016.01.023
更新日期:2016-03-01 00:00:00
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journal_title:Pharmacological research
pub_type: 杂志文章,评审
doi:10.1006/phrs.1998.0416
更新日期:1999-03-01 00:00:00
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journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/j.phrs.2015.03.017
更新日期:2015-05-01 00:00:00
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journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/s1043-6618(05)80116-1
更新日期:1991-02-01 00:00:00
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journal_title:Pharmacological research
pub_type: 临床试验,杂志文章
doi:10.1016/j.phrs.2019.104319
更新日期:2019-08-01 00:00:00