当前位置： SCI文献检索 > Experimental and Therapeutic Medicine期刊下所有文献 > MicroRNA-490-3p regulates cell proliferation and apoptosis in gastric cancer via direct targeting of AKT1.

MicroRNA-490-3p regulates cell proliferation and apoptosis in gastric cancer via direct targeting of AKT1.

Abstract：

:microRNA (miRNA) expression profiles of gastric cancer (GC) and adjacent healthy gastric mucosa tissue were used to search for differentially expressed miRNAs, identifying downregulated miRNA-490-3p in GC. The present study aimed to investigate the cellular function of miRNA-490-3p and its underlying mechanism in the occurrence and progression of GC. Reverse transcription-quantitative polymerase chain reaction was used to measure miRNA-490-3p expression levels in GC tissue and adjacent healthy tissue samples. The regulatory effect of miRNA-490-3p on the proliferation and apoptosis of GC cells was detected by cell counting kit-8, colony formation assay and flow cytometry. Bioinformatic methods were used to predict AKT1 as the target of miRNA-490-3p and this was verified by a dual-luciferase reporter assay. Furthermore, western blot analysis was used to measure protein expression of AKT1 in GC cells following overexpression or knockdown of miRNA-490-3p. The present study demonstrated that miRNA-490-3p expression was downregulated in GC tissue, compared with adjacent healthy tissue. In particular, miRNA-490-3p expression levels were significantly decreased in GC tissue samples from patients with advanced cancer (stage III+IV) compared with samples from patients with early-stage (stage I+II) cancer. Additionally, miRNA-490-3p expression levels were significantly decreased in GC tissue samples from patients whose tumor size was >3 cm, compared with those <3 cm. In vitro, downregulation of miRNA-490-3p promoted cell proliferation and suppressed apoptosis. In addition, rescue experiments demonstrated that overexpression of AKT1 partially reversed the effect of miRNA-490-3p on cell proliferation and apoptosis. The present study demonstrated that miRNA-490-3p regulated proliferation and apoptosis in gastric cancer cells via direct targeting of AKT1.

journal_name

Exp Ther Med

journal_title

Experimental and therapeutic medicine

authors

Yu H,Sun J,Jiang S,Xu Y

doi

10.3892/etm.2018.7042

subject

Has Abstract

pub_date

2019-02-01 00:00:00

pages

1330-1336

issue

eissn

1792-0981

issn

1792-1015

pii

ETM-0-0-7042

journal_volume

pub_type

杂志文章

在线工具

MicroRNA-490-3p regulates cell proliferation and apoptosis in gastric cancer via direct targeting of AKT1.