Notch3 and its CADASIL mutants differentially regulate cellular phenotypes.

Abstract:

:Notch3 is a member of the Notch family and its mutations are known to cause a hereditary human disorder called cerebral autosomal-dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). However, the specific function and signaling cascade initiated by CADASIL mutants remain unknown. To gain further insight into mechanism of action of CADASIL mutants, the present study conducted several experiments on the effects of Notch3 mutants in multiple cell lines. The protein levels of Notch3, fibronectin, collagen, inducible nitric oxide synthase and DNA (cytosine-5)-methyltransferase 1 (DNMT1) were determined by western blotting. The mRNA levels of IL-1β and TNF-α were measured by reverse transcription semi-quantitative PCR and DNMT1 mRNA levels were determined by quantitative PCR. Trypan blue staining was used for proliferation analysis and wound healing assays were performed to determine cell migration capability. The present study reported that R90C and R169C Notch3 mutants, and wild-type Notch3 had different effects on several cell lines. In T/GHA-VSMC cells, following the transfection of the two mutants, collagen and fibronectin expression increased, whereas expression decreased in IMR-90 cells. In BV2 cells, the two mutants resulted in decreased nitric oxide and iNOS production. In HeLa cells, proliferation and migration increased significantly following the transfection of the two mutants, whereas in the MCF-7 and HCC1937 cell lines, cell proliferation and migration decreased. In addition, the two mutants suppressed the expression of DNMT1 in HeLa and IMR-90 cells. Overall, the present study provided novel insights that further explored the underlying mechanisms of CADASIL.

journal_name

Exp Ther Med

authors

Lin C,Huang Z,Zhou R,Zhou Y,Shentu Y,Yu K,Zhang Y

doi

10.3892/etm.2020.9549

subject

Has Abstract

pub_date

2021-02-01 00:00:00

pages

117

issue

2

eissn

1792-0981

issn

1792-1015

pii

ETM-0-0-09549

journal_volume

21

pub_type

杂志文章
  • Platelet-derived growth factor-BB promotes proliferation and migration of retinal microvascular pericytes by up-regulating the expression of C-X-C chemokine receptor types 4.

    abstract::Stromal cell-derived growth factor (SDF)-1α acts as a ligand to C-X-C chemokine receptors 4 (CXCR4) and 7 (CXCR7), which are involved in the formation of choroidal neovascularization. Previous studies have demonstrated crosstalk between the platelet-derived growth factor (PDGF)-BB/PDGF receptor (PDGFR)-β and SDF-1α/CX...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2019.8016

    authors: Xiang DN,Feng YF,Wang J,Zhang X,Shen JJ,Zou R,Yuan YZ

    更新日期:2019-11-01 00:00:00

  • In vivo imaging of adipose-derived mesenchymal stem cells in female nude mice after simulated childbirth injury.

    abstract::The aim of the present study was to track in vivo the distribution and survival of adipose-derived mesenchymal stem cells (ASCs) transplanted into female BALB/c nude mice following simulated childbirth injury, using green fluorescent protein and luciferase dual labeling, bioluminescent imaging (BLI) and histological e...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2014.2092

    authors: Dai M,Xu P,Hou M,Teng Y,Wu Q

    更新日期:2015-02-01 00:00:00

  • Effect of mouse nerve growth factor on the expression of glial fibrillary acidic protein in hippocampus of neonatal rats with hypoxic-ischemic brain damage.

    abstract::The present study aimed to investigate the influence of mouse nerve growth factor (mNGF) on glial fibrillary acidic protein (GFAP) expression in neonatal rats with hypoxic-ischemic brain damage (HIBD). A total of 60 7-day-old neonatal rats were randomly divided into control, HIBD and mNGF groups (n=20). The rats in th...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2012.827

    authors: Yin X,Dong L,Wei W,Wang Y,Chai Y,Feng Z

    更新日期:2013-02-01 00:00:00

  • Clinical value of the correlations of mural coronary artery compression extent with myocardial bridge length and thickness evaluated by 128-slice CT.

    abstract::This study aimed to investigate the correlations between the detection rate of mural coronary artery (MCA) by 128-slice CT and the MCA compression extent in systole with myocardial bridge (MB) length and thickness. A retrospective analysis was conducted for 580 patients receiving multislicespiral CT coronary angiograp...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2012.879

    authors: Niu YJ,Zhang XL,Cao AD,Leng B

    更新日期:2013-03-01 00:00:00

  • Effects of single-session dietary counseling by dieticians on salt reduction in cardiology outpatients who consumed large amounts of salt.

    abstract::The purpose of the present study was to assess the effects of single-session dietary counseling on salt restriction in Japanese cardiology outpatients as assessed using spot urine measurements. A total of 72 patients (73±11 years old, including 30 females) who visited a cardiology outpatient clinic and had a salt inta...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2015.2452

    authors: Yamasaki T,Sadanaga T,Hirota S

    更新日期:2015-07-01 00:00:00

  • Characterization of novel intramedullary nailing method for treating femoral shaft fracture through finite element analysis.

    abstract::Femoral shaft fracture is one of the most common types of fracture encountered in the clinic. For certain complex femoral shaft fractures, the traditional intramedullary nail may not provide sufficient stability. Therefore, novel intramedullary nail systems are required. The femur 3D model and the internal fixation mo...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2020.8763

    authors: Cui Y,Xing W,Pan Z,Kong Z,Sun L,Sun L,Cheng X,Liu C

    更新日期:2020-08-01 00:00:00

  • Insight into the binding interaction of kaempferol-7-O-α-L-rhamnopyranoside with human serum albumin by multiple fluorescence spectroscopy and molecular modeling.

    abstract::Human serum albumin (HSA) is a transporting protein that has multiple functions. The binding interaction between HSA and small molecules affects its function and efficacy of small molecules. The present study reports that kaempferol-7-O-α-L-rhamnopyranoside (KR) interacts with HSA as indicated by multiple fluorescence...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2017.4427

    authors: Zhang W,Chen C,Zhang C,Duan J,Yao H,Li Y,Meng A,Shi J

    更新日期:2017-06-01 00:00:00

  • Laryngeal schwannoma excised under a microlaryngoscope without tracheotomy: A case report.

    abstract::Schwannomas are benign encapsulated tumors arising from Schwann cells in the peripheral nervous system. Between 25 and 45% of schwannomas occur in the head and neck region. Schwannomas of the larynx are extremely rare with an incidence of 0.1-1.5% in all benign laryngeal tumors. Laryngeal schwannomas usually occur in ...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2014.1528

    authors: Wang B,Dong P,Shen B,Xu H,Zheng J

    更新日期:2014-04-01 00:00:00

  • Identification of key gene modules and transcription factors for human osteoarthritis by weighted gene co-expression network analysis.

    abstract::Osteoarthritis (OA) is one of the most prevalent causes of joint disease. However, the pathological mechanisms of OA have remained to be completely elucidated, and further investigation into the underlying mechanisms of OA development and the identification of novel therapeutic targets are urgently required. In the pr...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2019.7848

    authors: Gao X,Sun Y,Li X

    更新日期:2019-10-01 00:00:00

  • Cyanidin ameliorates cisplatin-induced cardiotoxicity via inhibition of ROS-mediated apoptosis.

    abstract::Oxidative stress and apoptosis serve an essential role in cisplatin-induced cardiotoxicity, which limits its clinical use, and increases the risk of cardiovascular disease. As a natural drug, the antioxidant and antitumor effects of cyanidin have been recognized, but its protective effect on cisplatin-induced cardiomy...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2017.5617

    authors: Qian P,Yan LJ,Li YQ,Yang HT,Duan HY,Wu JT,Fan XW,Wang SL

    更新日期:2018-02-01 00:00:00

  • A compound heterozygous mutation of the alkaline phosphatase ALPL gene causes hypophosphatasia in a Han Chinese family.

    abstract::Hypophosphatasia (HPP) is a rare hereditary systemic disease that is characterized by defective bone and/or dental mineralization, and is caused by mutations in the alkaline phosphatase gene (ALPL). The present study investigated the ALPL mutation in a Chinese Han family with HPP and studied the pathogenesis of the mu...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2020.9281

    authors: Huang H,Wang J,Liang Y,Wei X,Guo D,Sun H,Zhang X,Xu X,Xiong F

    更新日期:2020-12-01 00:00:00

  • Association between the M235T polymorphism of the AGT gene and cytokines in patients with hypertension.

    abstract::The aim of the present study was to explore the association between the M235T polymorphism of the angiotensinogen (AGT) gene and cytokines in patients with essential hypertension (EH). A total of 300 patients with EH and an age-matched control group of 150 individuals without EH, secondary hypertension, myocardial inf...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2011.433

    authors: Cheng JL,Wang AL,Wan J

    更新日期:2012-03-01 00:00:00

  • A comparison study between CT angiography with 64-multislice spiral computed tomography and selective X-ray coronary angiography.

    abstract::The aim of this study was to evaluate the diagnostic value of 64-multislice spiral computed tomography (64-MSCT) for coronary stenosis compared with selective X-ray coronary angiography (SCA). Patients with chest pain, chest tightness or coronary stenosis received SCA and they acted as the controls. The sensitivity an...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2013.905

    authors: Cheng L,Jing S,Zhang Y

    更新日期:2013-03-01 00:00:00

  • Gestational weight management and pregnancy outcomes among women of advanced maternal age.

    abstract::In this study, we assessed the effects of pre-pregnancy body mass index (BMI) and gestational weight gain (GWG) on the pregnancy outcomes of women of advanced age using a back-propagation (BP) artificial neural network. We conducted a retrospective analysis on postpartum and hospital delivery data from 1,015 women of ...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2019.7752

    authors: Lin J,Fu Y,Han Q,Yan J,Chen R,Zhang H

    更新日期:2019-09-01 00:00:00

  • Agnogenic massive pulmonary embolism with syncope as initial symptom: A case report and review of the literature.

    abstract::Pulmonary embolism may escape prompt diagnosis since clinical symptoms and signs are nonspecific. The occurrence of syncope as the sole initial symptom in a previously healthy patient with no predisposing factors to embolism and no hemodynamic instability is extremely rare, which may have been a factor in the delayed ...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2013.992

    authors: Jiang AG,Lu HY

    更新日期:2013-05-01 00:00:00

  • MicroRNA-148b inhibits proliferation and the epithelial-mesenchymal transition and increases radiosensitivity in non-small cell lung carcinomas by regulating ROCK1.

    abstract::Non-small cell lung cancer (NSCLC) accounts for ~80% of all types of lung cancer, which has the highest morbidity and mortality of all types of cancer worldwide. It is important to identify novel biomarkers and the molecular mechanism of NSCLC to improve current treatments of NSCLC. The present study aimed to investig...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2018.5845

    authors: Luo H,Liang C

    更新日期:2018-04-01 00:00:00

  • Decreased microRNA-140-5p contributes to respiratory syncytial virus disease through targeting Toll-like receptor 4.

    abstract::The abnormal expression of miRNAs (miRs) has previously been reported in respiratory syncytial virus (RSV) disease. However, to the best of our knowledge, the expression of miR-140-5p in patients with an RSV infection has never been explored. Reverse transcription-quantitative polymerase chain reaction was performed t...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2018.6272

    authors: Zhang Y,Shao L

    更新日期:2018-08-01 00:00:00

  • Association of epidermal growth factor receptor and K-Ras mutations with smoking history in non-small cell lung cancer patients.

    abstract::Lung cancer, a major health problem affecting the epithelial lining of the lower respiratory tract, is considered to be one of the deadliest types of cancer in males and females and it is well-known that smoking is the chief cause of lung cancer. In addition to smoking and environmental factors, genetic susceptibility...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2012.829

    authors: Baykara O,Tansarikaya M,Demirkaya A,Kaynak K,Tanju S,Toker A,Buyru N

    更新日期:2013-02-01 00:00:00

  • Polyethylene glycol 4000 treatment for children with constipation: A randomized comparative multicenter study.

    abstract::The aim of this study was to evaluate the efficacy and safety of polyethylene glycol 4000 (PEG 4000) for the treatment of constipation in children over 8 years of age. A total of 216 children from 7 hospitals were enrolled. A total of 105 patients received oral PEG 4000 (20 g/day) and 111 patients received oral lactul...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2012.491

    authors: Wang Y,Wang B,Jiang X,Jiang M,Xu C,Shao C,Jia L,Huang Z,Xu X,Liu H,Shang L

    更新日期:2012-05-01 00:00:00

  • Capillary loss reflects disease activity and prognosis in patients with systemic sclerosis.

    abstract::Capillary density on nailfold capillaroscopy (NFC) is considered a promising instrument for assessing disease characteristics in patients with systemic sclerosis (SSc), however, there is no agreement yet over how to analyze and interpret the results. The objective of this study was to investigate the possible associat...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2020.8979

    authors: Gheorghiu AM,Oneata R,Ancuta I,Enuica A,Linte O,Macovei L,Bojinca M,Stoica V,Mihai C

    更新日期:2020-10-01 00:00:00

  • MicroRNA-135a-5p is involved in osteoporosis progression through regulation of osteogenic differentiation by targeting RUNX2.

    abstract::A number of microRNAs (miRs) have been revealed to be involved in the development of osteoporosis, including postmenopausal osteoporosis. The aim of the present study was to investigate miR-135a-5p expression and the cellular function of miR-135a-5p and its underlying mechanism in postmenopausal osteoporosis. miR-135a...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2019.7849

    authors: Shi X,Zhang Z

    更新日期:2019-10-01 00:00:00

  • Analysis of the efficacy of resveratrol treatment in patients with scarred uterus.

    abstract::Scarred uterus is caused by cesarean section surgery, and this condition affects further gestation and delivery in patients. Previous evidence suggested that resveratrol, a polyphenol compound, presents beneficial outcomes for patients with scarred uterus. Therefore, the aim of the present study was to analyze the eff...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2018.6126

    authors: Ma H,Qiao Z

    更新日期:2018-06-01 00:00:00

  • Expression variations and clinical significance of MMP-1, MMP-2 and inflammatory factors in serum of patients with deep venous thrombosis of lower extremity.

    abstract::Expression levels and clinical significance of matrix metalloproteinase-1 (MMP1), MMP-2 and inflammatory factors in the serum of patients with deep venous thrombosis (DVT) of lower extremity were investigated. Fifty untreated DVT patients were selected as the DVT group, and 50 patients undergoing health examination we...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2018.6922

    authors: Zhang T,Li Q,Wang L,Li G

    更新日期:2019-01-01 00:00:00

  • Overexpression of long non-coding RNA zinc finger antisense 1 in acute myeloid leukemia cell lines influences cell growth and apoptosis.

    abstract::The association between long non-coding RNA zinc finger antisense 1 (ZFAS1) and acute myeloid leukemia (AML) has not yet been investigated. The present study aimed to assess the potential role of ZFAS1 in AML cell proliferation and apoptosis. The expression of ZFAS1 mRNA in various AML cell lines (HL-60, KG-1, ML-1 an...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2017.4535

    authors: Guo H,Wu L,Zhao P,Feng A

    更新日期:2017-07-01 00:00:00

  • MicroRNA-490-3p regulates cell proliferation and apoptosis in gastric cancer via direct targeting of AKT1.

    abstract::microRNA (miRNA) expression profiles of gastric cancer (GC) and adjacent healthy gastric mucosa tissue were used to search for differentially expressed miRNAs, identifying downregulated miRNA-490-3p in GC. The present study aimed to investigate the cellular function of miRNA-490-3p and its underlying mechanism in the ...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2018.7042

    authors: Yu H,Sun J,Jiang S,Xu Y

    更新日期:2019-02-01 00:00:00

  • Surgical treatment of incarcerated calculi via laparoscopic bile duct exploration using laparotomy biliary lithotomy forceps.

    abstract::The present study aimed to investigate the practicability and clinical value of applying laparotomy biliary lithotomy forceps to laparoscopic bile duct exploration (LCBDE) for the surgical treatment of incarcerated calculi. A total of 63 patients were diagnosed with cholecystolithiasis and choledocholithiasis. The pre...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2016.3618

    authors: Jiang H,Wang SY,Jin XL,Jin JC,Gu HB,Zhang FM

    更新日期:2016-10-01 00:00:00

  • Validation of a novel UPLC-HRMS method for human whole-blood cyclosporine and comparison with a CMIA immunoassay.

    abstract::Therapeutic drug monitoring is an essential tool when managing the therapeutic use of immunosuppressant cyclosporine A (CsA) in cases with solid organ transplantation. In China, the concentration of CsA is primarily measured using immunoassays. However, existing literature recommends mass spectrometry as the current g...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2021.9623

    authors: Wang X,Qin W,Chen W,Liu H,Zhang D,Zhang X,Li P

    更新日期:2021-03-01 00:00:00

  • Utility of indocyanine green injection in patients with cervical cancer besides the identification of sentinel lymph node (Review).

    abstract::Due to the wide introduction of screening tests, patients with neoplastic diseases of the uterine cervix trend to be diagnosed in early stages of the disease, and less invasive surgical procedures are needed in certain cases. In this respect, the technique of sentinel lymph node dissection has been widely implemented ...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章,评审

    doi:10.3892/etm.2020.9095

    authors: Bacalbasa N,Balescu I,Diaconu C,Savu C,Savu C,Neacsu A,Belu E,Bratu O,Cretoiu D,Halmaciu I,Iliescu L,Balalau C,Filipescu A,Vilcu M,Brezean I

    更新日期:2020-10-01 00:00:00

  • Single administration of vildagliptin attenuates postprandial hypertriglyceridemia and endothelial dysfunction in normoglycemic individuals.

    abstract::Postprandial hypertriglyceridemia impairs endothelial function and plays an important role in the development of atherosclerosis. The aim of the present study was to examine the postprandial effects of the dipeptidyl peptidase-4 inhibitor vildagliptin and the α-glucosidase inhibitor voglibose on endothelial dysfunctio...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2014.2051

    authors: Noguchi K,Hirota M,Miyoshi T,Tani Y,Noda Y,Ito H,Nanba S

    更新日期:2015-01-01 00:00:00

  • Allicin activates autophagic cell death to alleviate the malignant development of thyroid cancer.

    abstract::Allicin has been reported to inhibit cancer cell proliferation, induce cell apoptosis and enhance the accumulation of reactive oxygen species. However, it has remained elusive whether allicin improves multidrug resistance in thyroid cancer cells through modulating autophagy. The present study demonstrated that combine...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2018.5828

    authors: Xiang Y,Zhao J,Zhao M,Wang K

    更新日期:2018-04-01 00:00:00