Abstract:
:Transcription of eukaryotic protein-coding genes requires passage of RNA polymerase II (Pol II) through nucleosomes, but it is unclear how this is achieved. Here we report the cryo-EM structure of transcribing Saccharomyces cerevisiae Pol II engaged with a downstream nucleosome core particle at an overall resolution of 4.4 Å. Pol II and the nucleosome are observed in a defined relative orientation that is not predicted. Pol II contacts both sides of the nucleosome dyad using its clamp head and lobe domains. Structural comparisons reveal that the elongation factors TFIIS, DSIF, NELF, SPT6, and PAF1 complex can be accommodated on the Pol II surface in the presence of the oriented nucleosome. Our results provide a starting point for analysing the mechanisms of chromatin transcription.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Farnung L,Vos SM,Cramer Pdoi
10.1038/s41467-018-07870-ysubject
Has Abstractpub_date
2018-12-21 00:00:00pages
5432issue
1issn
2041-1723pii
10.1038/s41467-018-07870-yjournal_volume
9pub_type
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