Abstract:
:To investigate the repair process of DNA damage induced by ionizing radiation in isolation from various types of cytoplasmic damage, we transfected X-irradiated enhanced green fluorescent protein (EGFP)-expressing plasmid DNA into non-irradiated mammalian cells using lipofectamine. The repair kinetics of the irradiated plasmids in the cells were visualized under microscopy as the EGFP fluorescence emitted by transfected cells. Using an agarose gel electrophoresis method, the yields of single- and double-strand breaks of the plasmids were also quantified. As positive control experiments, plasmid DNA with single- or double-strand breaks induced by a nicking or restriction enzyme were also transfected into the cells. The DNA repair rates for X-ray-irradiated plasmids were significantly lower than those of the enzymatically digested positive control samples. These results indicate that X-rays could induce less repairable damage than that induced by enzymes.
journal_name
Radiat Prot Dosimetryjournal_title
Radiation protection dosimetryauthors
Nakaue H,Obata Y,Kaminaga K,Akimitsu N,Yokoya Adoi
10.1093/rpd/ncy241subject
Has Abstractpub_date
2019-05-01 00:00:00pages
79-83issue
1-2eissn
0144-8420issn
1742-3406pii
5244130journal_volume
183pub_type
杂志文章abstract::The material for this study comprised control protocols from 248 mammography screening facilities, prepared by physicists employed at 16 Regional Coordinating Centres and the results from the clinical evaluation of mammographic images in 248 facilities in Poland. All mammograms were evaluated independently by three ex...
journal_title:Radiation protection dosimetry
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journal_title:Radiation protection dosimetry
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journal_title:Radiation protection dosimetry
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