Abstract:
INTRODUCTION:A growing body of evidence indicates that brain cytokines are involved in the control of the cardiovascular system. Tumour necrosis factor (TNF) is an archetypal cytokine, which exerts its proinflammatory actions via type 1 receptor (TNFR1). Interleukin 10 (IL-10) plays a critical anti-inflammatory role by binding to its receptor (IL-10Ra). The orchestrated inflammatory response is largely dependent on an intricate balance between proinflammatory and anti-inflammatory cytokines and expression of their receptors. AIM:In the study we evaluated the expression of the cytokines and their receptors in the brains of spontaneously hypertensive (SH) and normotensive Wistar-Kyoto (WKY) rats, and how the cytokines affect arterial blood pressure. METHODS:In SH and WKY rats we recorded systolic blood pressure with tail cuff method and measured concentration of TNF, IL-10, TNFR1, and IL-10Ra in the serum, the brainstem, and the hypothalamus; we also measured serum concentrations of copeptin, a surrogate of vasopressin release, angiotensin II and norepinephrine. We immunostained brainstem sections for TNFR1, IL-10Ra, neurons, astrocytes and microglia for confocal imaging. In urethane anaesthetized SH and WKY rats, we invasively recorded blood pressure response to intracerebroventricular (IVC) infusion of TNF or IL-10. We also pharmacologically evaluated baroreflex with phenylephrine and chemoreflex with cyanide in SH and WKY rats. RESULTS:Compared to WKY rats, SH rats had: (1) higher blood pressure; (2) blunted baroreflex and augmented peripheral chemoreflex; (3) greater pressor response to ICV infused TNF and greater hypotensive response to ICV infused IL-10; (4) higher concentration of TNF in the ventral and dorsal aspects of the medulla oblongata; (5) higher expression of TNFR1 in the dorsal medulla; (6) higher concentration of IL-10 in both aspects of the medulla; (7) lower expression of IL-10Ra in the dorsal medulla. Confocal imaging showed co-localization of TNFR1 and IL-10Ra with neurons, astrocytes and microglia in both SH and WKY rats. The concentration of the cytokines and their receptors were significantly higher in the brain than in the serum. There were no significant differences in the concentration of the cytokines and their receptors in the hypothalamic region and in the serum between SH and WKY rats. Serum concentrations of norepinephrine, angiotensin II and copeptin were similar between SH and WKY rats. CONCLUSIONS:Taken together, these findings suggest the presence of a potent milieu for effective TNF signalling in the brainstem, which is associated with the hypertensive phenotype and enhanced hemodynamic response to intrabrain administration of the cytokines. In addition, we hypothesize that the increased IL-10 concentration in the brainstem is a compensatory mechanism for the upregulated TNF system.
journal_name
Cytokinejournal_title
Cytokineauthors
Segiet A,Smykiewicz P,Kwiatkowski P,Żera Tdoi
10.1016/j.cyto.2018.07.003subject
Has Abstractpub_date
2019-01-01 00:00:00pages
185-194eissn
1043-4666issn
1096-0023pii
S1043-4666(18)30290-4journal_volume
113pub_type
杂志文章相关文献
CYTOKINE文献大全abstract::Cytokine induction following stimulation by endotoxin (LPS) was investigated in human peripheral whole blood. Blood cells were suspended in autologous plasma diluted in basal medium (BM-plasma) or Compound Sodium Lactate Solution (CSL-plasma), or in autologous serum diluted in CSL. Induction of interleukin 1 beta, int...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/1043-4666(94)90020-5
更新日期:1994-05-01 00:00:00
abstract::We investigated the effects of either intravenous (IV) or intrabronchial (IB) treatment with transforming growth factor beta1 (TGF-beta1) during bacterial pneumonia in rats. Immediately following IB Escherichia coli inoculation (T0), animals (n=270) were randomized to receive a single treatment with human recombinant ...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2003.07.003
更新日期:2003-11-21 00:00:00
abstract::Recently, a novel technique for "real time" quantitative Reverse Transcriptase-PCR which measures PCR-product accumulation during the exponential phase of the PCR reaction using a dual-labelled fluorogenic probe, has been developed. This method allows direct detection of PCR-product formation by measuring the increase...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.1998.0426
更新日期:1999-04-01 00:00:00
abstract::The aim of the study was to characterize and to quantify peripheral and tissue. IL-35- and IL-37-producing cells in Inflammatory Bowel Disease (IBD) patients. We studied a total of 38 active UC, 31 inactive UC, 17 active CD, and 13 inactive CD and 50 non-inflamed tissues as control group. Gene expression was measured ...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2015.04.009
更新日期:2015-10-01 00:00:00
abstract::Treatment of non-small cell lung carcinoma (NSCLC) remains at a disappointingly low success rate. Not only is metastatic spread common in NSCLC, but therapeutic success decreases dramatically once metastases are present. Understanding factors which contribute to poor prognosis in NSCLC is critical for development of m...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2009.11.015
更新日期:2010-03-01 00:00:00
abstract::Burn injury is associated with profound inflammation and activation of the innate immune system involving gammadelta T-cells. Similarly, Toll-like receptors (TLR) are associated with activation of the innate immune response; however, it is unclear whether TLR expression is altered in gammadelta T-cells after major bur...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2008.09.001
更新日期:2008-12-01 00:00:00
abstract::Angiopoietin-like proteins (ANGPTL) are responsible for inhibiting lipoprotein lipase activity, and ANGPTL3 and ANGPTL4 deficiencies have been shown to lower lipoprotein levels in animal models and in humans carrying loss-of-function mutations. Sickle cell anemia (SCA) is a hereditary hemolytic anemia characterized by...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2019.04.014
更新日期:2019-08-01 00:00:00
abstract::In order to study structure-function relationships of the M(r) 55,000 subunit of the murine IL-2R (p55 IL-2R) by epitope mapping, we have expressed the p55 IL-2R molecule in a cell-free translation system. Under these in vitro conditions, we detected the expected p55 IL-2R polypeptide initiated at Met 1 and, in additi...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/1043-4666(92)90066-z
更新日期:1992-07-01 00:00:00
abstract::We examined the possible alteration of circulating transforming growth factor-beta1 (TGF-beta1) concentrations in a time-dependent fashion in human plasma. Plasma TGF-beta1 was measured three times at 2 week-intervals from each of 12 healthy participants. Platelet factor 4 (PF4) was measured in parallel with TGF-beta1...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.2001.0895
更新日期:2001-11-07 00:00:00
abstract::CCR2, and its principle ligand MCP-1/CCL2, have been well documented for their ability to induce monocyte infiltration and promote the pathogenesis of rheumatoid arthritis and atherosclerosis. In order to assess additional roles for CCR2, we inserted allogeneic implants into CCR2-/- and MCP-1-/- mice and characterized...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.2002.1031
更新日期:2002-05-21 00:00:00
abstract::Within the human TNF promoter we have identified two sites at positions -189 and -101 that show C/EBP specific binding of nuclear proteins from cells of the human monocytic line Mono Mac 6. Supershift analysis with anti C/EBP antibodies revealed that the complexes formed consist of both C/EBP alpha and C/EBP beta. Whe...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.1996.0046
更新日期:1996-05-01 00:00:00
abstract::Human interferon regulatory factor 7 (IRF7) plays an important role in the innate antiviral immune response. To date, the characteristics and functions of waterfowl IRF7 have not been clarified. This study reports the cDNA sequence, tissue distribution, and antiviral function of duck IRF7. The duck IRF7 gene has a 153...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2018.06.001
更新日期:2019-01-01 00:00:00
abstract::The interleukin (IL)-7 receptor (IL-7R) is expressed on human pre-B but not mature B-cells. We hypothesised that aberrant expression of IL-7R contributes to B-cell oncogenesis. Surface expression of IL-7R components CD127 and CD132, and intracellular Ki-67 and Bcl-2 were examined by flow cytometry on peripheral blood ...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2009.12.001
更新日期:2010-04-01 00:00:00
abstract::Since liver regeneration after partial hepatectomy (PHx) is known to improve by pretreatment with recombinant human G-CSF (rhG-CSF), we investigated the mechanism by evaluating the distribution and activity of sinusoidal NK cells. F344 rats were treated with rhG-CSF (250 microg/kg/day) for 5 days before PHx. Pretreatm...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2006.04.001
更新日期:2006-04-01 00:00:00
abstract::Non-Hodgkin's lymphomas (NHL) constitute a heterogenous group of mainly B-cell lymphoproliferative diseases with different patterns of clinical behaviour. Biological mechanisms leading to development of NHL are not clearly understood. Transforming growth factor-beta1 (TGF-beta1) influences B cell growth and developmen...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2005.12.010
更新日期:2006-02-07 00:00:00
abstract::We investigated the effects of human interleukin 10 (IL-10) on the antibacterial and antifungal activities of human neutrophils (PMNs) against Staphylococcus aureus and Candida albicans. Incubation of PMNs from healthy volunteers with 20-100 ng/ml of IL-10 at 37 degrees C for 1 h suppressed phagocytosis of serum-opson...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.1999.0567
更新日期:2000-04-01 00:00:00
abstract::The purpose of these studies was to test whether pentoxifylline, a drug that can inhibit the production and action of cytokines hypothesized to be endogenous pyrogens (for example, interleukin 1 and tumor necrosis factor [TNF]), is antipyretic. We also tested the effects of pentoxifylline on plasma activities of inter...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/1043-4666(90)90032-o
更新日期:1990-07-01 00:00:00
abstract:OBJECTIVE:The effects of LDL-apheresis (LDLa) with dextran sulphate on plasma cytokines in 6 homozygous familial hypercholesterolemic (HozFH) patients, were evaluated. METHODS:Plasma IL-1α; IL-1ra; IL-4; IL-6; IL-10; IL-12(p40); IL-12(p70); TNF-α, sTNF-R, VEGF, VEGF-R1, E-Selectin (ESEL), and P-Selectin (PSEL) concent...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2011.04.003
更新日期:2011-08-01 00:00:00
abstract::This study was conducted to compare the secretion of TGF-beta isoforms by human ovarian carcinoma (OVCA) cell lines (n=12) and human peritoneal mesothelial cells (HPMC;n=6) and to examine the regulation of their production by inflammatory cytokines. TGF-beta isoforms were furthermore analysed in OVCA-associated asciti...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.1999.0632
更新日期:2000-07-01 00:00:00
abstract:BACKGROUND:Vascular endothelial growth factor (VEGF) is the key regulator of angiogenesis in the development of various cancers. Previous studies have examined the relationship between VEGF gene promoter polymorphisms such as -2578C/A and -460C/T and bladder cancer risk; however, these results are inconclusive. Therefo...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2020.155112
更新日期:2020-07-01 00:00:00
abstract:OBJECTIVES:To investigate the distribution of multiple cytokines in gastroschisis and reveal its association with clinical outcomes, including gastrointestinal disorders and fetal brain damage caused by chronic inflammation in gastroschisis. METHODS:We obtained amniotic fluid and arterial cord blood from 10 patients w...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2020.155406
更新日期:2021-02-01 00:00:00
abstract::Parasites of the genus Leishmania cause the disease leishmaniasis. As the sandfly vector transfers the promastigotes into the skin of the human host, the infection is either cured or exacerbated. In the process, there emerge several unsolved paradoxes of leishmaniasis. Chronologically, as the infections starts in skin...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2020.155304
更新日期:2020-09-29 00:00:00
abstract:BACKGROUND:Why severity varies in blastomycosis outbreaks remains unresolved. In experimental pulmonary blastomycosis, susceptibility varied in mouse strains. In susceptible BALB/c the response is Th-2, in immunized, resistance is associated with Th-1. Can susceptibility be redirected by IL-12? Methods, results: BALB/c...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2006.08.004
更新日期:2006-09-01 00:00:00
abstract::Acute respiratory distress syndrome following an acute lung injury (ALI) is a life threatening inflammatory condition predominantly characterized by vascular protein leakage, neutrophil recruitment and overexpression of proinflammatory cytokines. Pulmonary and systemic bacterial infections are the major cause of ALI w...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2020.155049
更新日期:2020-05-01 00:00:00
abstract::Cytotoxic action of leukocytes may be involved in the pathogenesis of inflammatory heart muscle disorders. We investigated the expression of rat leukocyte chemotactic cytokines--cytokine induced neutrophil chemoattractant (CINC) and JE--in cultured neonatal rat cardiac myocytes; CINC belongs to the interleukin 8 (IL-8...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.1995.0037
更新日期:1995-04-01 00:00:00
abstract::LIGHT (lymphotoxin-like inducible protein that competes with glycoprotein D for binding herpesvirus entry mediator on T cells) is a recently identified of the tumor necrosis factor alpha (TNF-alpha) ligand superfamily. We wanted to establish whether the presence of chronic viral hepatitis could be implicated in enhanc...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2007.07.189
更新日期:2007-09-01 00:00:00
abstract::Immunoincompetence is a profound problem in end-stage renal failure patients undergoing hemodialysis, and chronic inflammation with altered serum levels of inflammation markers has been reported. Gene expression patterns have had little relevance for leukocyte research so far because of limitations in transcript level...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2004.12.011
更新日期:2005-05-07 00:00:00
abstract::The objectives were to determine if blood IL-10 levels rise during the early stages of acute (wasting) pre-pubescent malnutrition in metabolically distinct murine models known to depress inflammatory immune competence and whether systemic IL-10 production is affected in these pathologies. Weanling C57BL/6J mice were a...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2009.05.015
更新日期:2009-08-01 00:00:00
abstract::Murine fibrosarcoma L929 cells were transfected with human Fas cDNA. The mode of cell death was analysed following treatment either with tumour necrosis factor (TNF) or with agonistic antibodies to Fas. While triggering of the TNF receptors led to necrosis, clustering of the Fas antigen resulted in apoptotic cell deat...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.1997.0252
更新日期:1997-11-01 00:00:00
abstract::Interleukin-17A (IL-17A) contributes to the pathogenesis of arthritis. Data from experimental arthritis indicate IL-17 receptor signaling as a critical pathway in turning an acute synovitis into a chronic destructive arthritis. The identification of six IL-17 family members (IL-17A-F) may extend the role of this novel...
journal_title:Cytokine
pub_type: 杂志文章,评审
doi:10.1016/j.cyto.2007.09.014
更新日期:2008-02-01 00:00:00