当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES期刊下所有文献 > Synthesis and characterization of a novel organosilane-functionalized chitosan nanocarrier as an efficient gene delivery system: Expression of green fluorescent protein.

Synthesis and characterization of a novel organosilane-functionalized chitosan nanocarrier as an efficient gene delivery system: Expression of green fluorescent protein.

Abstract：

:Semi-essential arginine amino acid was selected to synthesis an organosilane linker for modifying chitosan biopolymer. The novel organosilane linker which was chemically synthesized by reaction of arginine with 3‑chloropropyl trimethoxy silane, covalently bonded to the chitosan amino group. The chemical structure of resulting nanocarrier was characterized by 1H NMR, wide-X-ray diffraction, TEM, FESEM and EDX. A maximum retardation capacity of the nanocarrier to the plox plasmid was obtained 3 at physiological pH (7.4). The mean of cell viability and cytotoxicity of the nanocarrier was determined 85% by MTT assay. In addition, the gene transfection of the nanocarrier was obtained top of 20% gene expression. The studies have shown very good DNase 1 enzyme protection of plasmid by the nanocarrier.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Kashkouli KI,Torkzadeh-Mahani M,Mosaddegh E

doi

10.1016/j.ijbiomac.2018.11.145

subject

Has Abstract

pub_date

2019-03-15 00:00:00

pages

143-148

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(18)33028-9

journal_volume

125

pub_type

杂志文章

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