Abstract：
:Sinapine is the main secondary metabolite present in rapeseed pomace (RSP) with its concentration being dependent on rapeseed processing, growing conditions, extraction parameters and the country of origin. Here we report, the concentration of sinapine from an extract of defatted RSP harvested in the North East of Scotland. Using liquid chromatography tandem mass spectrometry, the most abundant phenolic compound in the RSP extract was, as expected, sinapine (109.1 mg/g RSP extract). Additionally, sinapic, caffeic, ferulic and syringic acids were identified (0.159-3.91 mg/g RSP extract). Sinapine together with the phenolics at the concentration present in the RSP extract, exhibited ≥50% activity relative to the extract in antioxidant assays. Furthermore, sinapine provided plasmid DNA (pBR322) protection, from 2,2'-azobis(2-amidinopropane) dihydrochloride and inhibited acetylcholinesterase activity by 85%. Molecular docking was utilised to explain the inhibitory activity. RSP can be an excellent source of bioactive compounds for pharmaceuticals, food additive and nutraceutical applications.
journal_name
Food Chemjournal_title
Food chemistryauthors
Yates K,Pohl F,Busch M,Mozer A,Watters L,Shiryaev A,Kong Thoo Lin Pdoi
10.1016/j.foodchem.2018.10.045subject
Has Abstractpub_date
2019-03-15 00:00:00pages
768-775eissn
0308-8146issn
1873-7072pii
S0308-8146(18)31811-9journal_volume
276pub_type
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