Abstract：
:In this work the development and in house validation of the HMF direct determination in corn and cane syrups by HPLC-UV was carried out for the first time. The separation was done with isocratic elution of a mobile phase comprising water (with 0.5% formic acid) and acetonitrile (90:10, v/v) on Phenomenex C18 column (5.0 μm, 4.6 × 150 mm), at 30 °C, flow rate of 0.8 mL min(-1) and detection at 285 nm. The validated method showed excellent performance with low limits (LOD and LOQ of 0.09 and 0.26 mg L(-1), respectively), good accuracy (recovery rates between 100% and 104%) and precision (RSD's for repeatability and intermediate precision between 0.57% and 6.43%). Good selectivity and linearity were also observed. HMF contents in both foods were very high (406.6-2121.3 mg kg(-1) for corn syrup and 109.2-893.1 mg kg(-1) for cane syrup), which arouses concern about food safety of these products.
journal_name
Food Chemjournal_title
Food chemistryauthors
de Andrade JK,Komatsu E,Perreault H,Torres YR,da Rosa MR,Felsner MLdoi
10.1016/j.foodchem.2015.05.131subject
Has Abstractpub_date
2016-01-01 00:00:00pages
481-486eissn
0308-8146issn
1873-7072pii
S0308-8146(15)00875-4journal_volume
190pub_type
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