High throughput screening and profiling of high-value carotenoids from a wide diversity of bacteria in surface seawater.

Abstract:

:Carotenoids are valuable natural colorants that exhibit numerous health promoting properties, and thus are widely used in food, feeds, pharmaceutical and nutraceuticals industries. In this study, we isolated and identified novel microbial sources that produced high-value carotenoids using high throughput screening (HTS). A total of 701 pigmented microbial strains library including marine bacteria and red yeast was constructed. Carotenoids profiling using HPLC-DAD-MS methods showed 88 marine bacterial strains with potential for the production of high-value carotenoids including astaxanthin (28 strains), zeaxanthin (21 strains), lutein (1 strains) and canthaxanthin (2 strains). A comprehensive 16S rRNA gene based phylogenetic analysis revealed that these strains can be classified into 30 species belonging to five bacterial classes (Flavobacteriia, α-Proteobacteria, γ-Proteobacteria, Actinobacteria and Bacilli). Importantly, we discovered novel producers of zeaxanthin and lutein, and a high diversity in both carotenoids and producing microbial strains, which are promising and highly selective biotechnological sources for high-value carotenoids.

journal_name

Food Chem

journal_title

Food chemistry

authors

Asker D

doi

10.1016/j.foodchem.2018.03.109

subject

Has Abstract

pub_date

2018-09-30 00:00:00

pages

103-111

eissn

0308-8146

issn

1873-7072

pii

S0308-8146(18)30551-X

journal_volume

261

pub_type

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