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Evaluation of the antioxidant activity of passion fruit (Passiflora edulis and Passiflora alata) extracts on stimulated neutrophils and myeloperoxidase activity assays.

Abstract：

:The antioxidant activity of methanol extracts from Passiflora edulis and Passiflora alata pulp, and P. edulis rinds, healthy or infected with the passion fruit woodiness virus (PWV), was investigated using the oxidant activities of the neutrophil and the neutrophil granule enzyme myeloperoxidase (MPO), both playing key roles in inflammation. The reactive oxygen species produced by stimulated neutrophils were evaluated by lucigenin-enhanced chemiluminescence (CL) and the activity of purified MPO was measured by SIEFED (Specific Immunological Extraction Followed by Enzymatic Detection), a technique for studying the direct interaction of a compound with the enzyme. The rind extracts of P. edulis possessed higher and dose-dependent inhibitory effects on CL response and on the peroxidase activity of MPO than total pulp extracts from both passion fruit species. The quantification of isoorientin in the extracts showed a correlation with their antioxidant activity, suggesting the potential of P. edulis rinds as functional food or as a possible source of natural flavonoids.

journal_name

Food Chem

journal_title

Food chemistry

authors

Zeraik ML,Serteyn D,Deby-Dupont G,Wauters JN,Tits M,Yariwake JH,Angenot L,Franck T

doi

10.1016/j.foodchem.2011.03.001

subject

Has Abstract

pub_date

2011-09-15 00:00:00

pages

259-65

issue

2

eissn

0308-8146

issn

1873-7072

pii

S0308-8146(11)00367-0

journal_volume

128

pub_type

杂志文章

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