Abstract:
:A new protein quantification method based on first order derivative spectrophotometry was established to eliminate various interferences, mainly chitosan, to the utmost using bovine serum albumin (BSA) as a model food protein. Absorbance spectra of BSA solutions were recorded and their first order derivative calculated. The values of derivative absorbance at 288 nm were used to generate linear calibration curve of BSA. The new method was applied in entrapping BSA into chitosan-tripolyphosphate beads. A general calibration curve was established with a CI (width of 95% confidence interval of three repeat measurements of unknown samples) less than 0.0262 g/L and a LOQ (limit of quantification) of 0.11 g/L, showing excellent tolerance to various interferences, which was further verified by the good mass balance of BSA during encapsulation. Overall, the method successfully eliminated the interferences from chitosan and other factors to facilitate the measurement of protein in complicated environments.
journal_name
Food Chemjournal_title
Food chemistryauthors
Yuan D,O'Riordan ED,Jacquier JCdoi
10.1016/j.foodchem.2019.02.121subject
Has Abstractpub_date
2019-08-15 00:00:00pages
1-6eissn
0308-8146issn
1873-7072pii
S0308-8146(19)30463-7journal_volume
289pub_type
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