Abstract:
:Bovine β-lactoglobulin (BLG) is the main allergen in cows' milk, and the most commonly used method for detecting BLG is enzyme-linked immunosorbent assay (ELISA). However, antibodies used in commercial ELISA kits do not recognize specifically BLG IgE epitopes. Here, an antibody specific to IgE linear epitopes for BLG was used to develop a sandwich ELISA using a rabbit anti-BLG polyclonal antibody. The linear range for BLG detection was 31.25-8000ng/mL and limit of detection was 1.96ng/mL. BLG content in dairy samples was determined, and there was a good agreement between this immunoassay and reversed-phase high-performance liquid chromatography with high recovery. Additionally, BLG content in food samples had an average recovery of 104.25%. Allergenic residues were also detected in hydrolyzed infant formulas. The method developed could be a practical approach to determine BLG and its allergenic residues in food with a high degree of sensitivity, reliability and recovery.
journal_name
Food Chemjournal_title
Food chemistryauthors
He S,Li X,Gao J,Tong P,Chen Hdoi
10.1016/j.foodchem.2017.01.060subject
Has Abstractpub_date
2017-07-15 00:00:00pages
33-40eissn
0308-8146issn
1873-7072pii
S0308-8146(17)30071-7journal_volume
227pub_type
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