Abstract:
:A sensitive and selective high performance liquid chromatography-tandem mass spectrometric method (HPLC-MS/MS) has been developed for simultaneous analysis of 11 components (7 flavonoids and 4 phenolic acids) in Herba Scutellariae barbatae. Simultaneous separation of these 11 compounds was achieved on a C18 analytical column with gradient elution of methanol and 0.1‰ acetic acid (v/v) at a flow rate of 0.8mL/min. The precursor and product ions of analytes were monitored on a hybrid quadrupole linear ion trap mass spectrometer equipped with a turbo ion spray interface in negative mode using multiple-reaction monitoring (MRM). Full validation of the assay was carried out including linearity, precision, accuracy, limits of detection and quantification. The results demonstrated that the method developed was reliable, rapid and specific. Then the method was successfully applied to differentiate 18 batches of Herba Scutellariae barbatae samples and 3 batches of its confused plants from different sources.
journal_name
Food Chemjournal_title
Food chemistryauthors
Qiao S,Shi R,Liu M,Zhang C,Yang W,Shi X,Jiang X,Wang C,Wang Qdoi
10.1016/j.foodchem.2011.05.064subject
Has Abstractpub_date
2011-12-01 00:00:00pages
1297-304issue
3eissn
0308-8146issn
1873-7072pii
S0308-8146(11)00767-9journal_volume
129pub_type
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