Caloric restriction promotes functional changes involving short-chain fatty acid biosynthesis in the rat gut microbiota.


:Caloric restriction (CR) is known to promote health and longevity, likely via modification of the gut microbiota (GM). However, functional and metabolic changes induced in the GM during CR are still unidentified. Here, we investigated the short- and long-term effects of CR on the rat GM using a metaproteogenomic approach. We show that a switch from ad libitum (AL) low fat diet to CR in young rats is able to induce rapid and deep changes in their GM metaproteomic profile, related to a reduction of the Firmicutes/Bacteroidetes ratio and an expansion of lactobacilli. Specifically, we observed a significant change in the expression of the microbial enzymes responsible for short-chain fatty acid biosynthesis, with CR boosting propionogenesis and limiting butyrogenesis and acetogenesis. Furthermore, these CR-induced effects were maintained up to adulthood and started to be reversed after a short-term diet change. We also found that CR alters the abundance of an array of host proteins released in stool, mainly related to epithelial barrier integrity and inflammation. Hence, our results provide thorough information about CR-induced modifications to GM and host functional activity, and might constitute the basis for novel GM-based approaches aimed at monitoring the effectiveness of dietary interventions.


Sci Rep


Scientific reports


Tanca A,Abbondio M,Palomba A,Fraumene C,Marongiu F,Serra M,Pagnozzi D,Laconi E,Uzzau S




Has Abstract


2018-10-03 00:00:00












  • Prediction of Alzheimer's disease using blood gene expression data.

    abstract::Identification of AD (Alzheimer's disease)-related genes obtained from blood samples is crucial for early AD diagnosis. We used three public datasets, ADNI, AddNeuroMed1 (ANM1), and ANM2, for this study. Five feature selection methods and five classifiers were used to curate AD-related genes and discriminate AD patien...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Lee T,Lee H

    更新日期:2020-02-26 00:00:00

  • Mixture toxicity assisting the design of eco-friendlier plant protection products: a case-study using a commercial herbicide combining nicosulfuron and terbuthylazine.

    abstract::The development of environmentally friendly plant protection products (PPPs), including pesticides, is a challenge nowadays. A commercial herbicide combining terbuthylazine and nicosulfuron as active substances (a.s.) was selected as a model PPP. The suitability of manipulating the ratio between a.s. towards alternati...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Queirós L,Vidal T,Nogueira AJA,Gonçalves FJM,Pereira JL

    更新日期:2018-04-03 00:00:00

  • Learning to find spatially reversed sounds.

    abstract::Adaptation to systematic visual distortions is well-documented but there is little evidence of similar adaptation to radical changes in audition. We use a pseudophone to transpose the sound streams arriving at the left and right ears, evaluating the perceptual effects it provokes and the possibility of learning to loc...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Bermejo F,Di Paolo EA,Gilberto LG,Lunati V,Barrios MV

    更新日期:2020-03-12 00:00:00

  • Traumatic Brain Injury Leads to Accelerated Atherosclerosis in Apolipoprotein E Deficient Mice.

    abstract::Traumatic brain injury (TBI) has been associated with atherosclerosis and cardiovascular mortality in humans. However the causal relationship between TBI and vascular disease is unclear. This study investigated the direct role of TBI on vascular disease using a murine model of atherosclerosis. Apolipoprotein E deficie...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Wang J,Su E,Wang H,Guo C,Lawrence DA,Eitzman DT

    更新日期:2018-04-04 00:00:00

  • Conserved-residue mutations in Wzy affect O-antigen polymerization and Wzz-mediated chain-length regulation in Pseudomonas aeruginosa PAO1.

    abstract::O antigen (O-Ag) in many bacteria is synthesized via the Wzx/Wzy-dependent pathway in which Wzy polymerizes lipid-linked O-Ag subunits to modal lengths regulated by Wzz. Characterization of 83 site-directed mutants of Wzy from Pseudomonas aeruginosa PAO1 (WzyPa) in topologically-mapped periplasmic (PL) and cytoplasmic...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Islam ST,Huszczynski SM,Nugent T,Gold AC,Lam JS

    更新日期:2013-12-06 00:00:00

  • Excess protein enabled dog domestication during severe Ice Age winters.

    abstract::Dogs (Canis familiaris) are the first animals to be domesticated by humans and the only ones domesticated by mobile hunter-gatherers. Wolves and humans were both persistent, pack hunters of large prey. They were species competing over resources in partially overlapping ecological niches and capable of killing each oth...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Lahtinen M,Clinnick D,Mannermaa K,Salonen JS,Viranta S

    更新日期:2021-01-07 00:00:00

  • Sex-Related Differences in Impact on Safety of Pharmacogenetic Profile for Colon Cancer Patients Treated with FOLFOX-4 or XELOX Adjuvant Chemotherapy.

    abstract::Polymorphisms contribute to inter-individual differences and show a promising predictive role for chemotherapy-related toxicity in colon cancer (CC). TOSCA is a multicentre, randomized, non-inferiority, phase III study conducted in high-risk stage II/stage III CC patients treated with 6 vs 3 months of FOLFOX-4 or XELO...

    journal_title:Scientific reports

    pub_type: 杂志文章,随机对照试验


    authors: Ruzzo A,Graziano F,Galli F,Galli F,Rulli E,Lonardi S,Ronzoni M,Massidda B,Zagonel V,Pella N,Mucciarini C,Labianca R,Ionta MT,Bagaloni I,Veltri E,Sozzi P,Barni S,Ricci V,Foltran L,Nicolini M,Biondi E,Bramati A,

    更新日期:2019-08-08 00:00:00

  • Cardiac endothelial cell-derived exosomes induce specific regulatory B cells.

    abstract::The mechanism of immune tolerance is to be further understood. The present study aims to investigate the role of the Cardiac endothelial cell (CEC)-derived exosomes in the induction of regulatory B cells. In this study, CECs were isolated from the mouse heart. Exosomes were purified from the culture supernatant of the...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Song J,Chen X,Wang M,Xing Y,Zheng Z,Hu S

    更新日期:2014-12-23 00:00:00

  • Cholesterol modulates acetylcholine receptor diffusion by tuning confinement sojourns and nanocluster stability.

    abstract::Translational motion of neurotransmitter receptors is key for determining receptor number at the synapse and hence, synaptic efficacy. We combine live-cell STORM superresolution microscopy of nicotinic acetylcholine receptor (nAChR) with single-particle tracking, mean-squared displacement (MSD), turning angle, ergodic...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Mosqueira A,Camino PA,Barrantes FJ

    更新日期:2018-08-10 00:00:00

  • Contribution of DNA methylation to the expression of FCGRT in human liver and myocardium.

    abstract::FcRn mediates recycling and transcytosis of IgG and albumin in various cell types. The MHC-class-I-like protein of the FcRn heterodimer is encoded by FCGRT. Few determinants of variable FCGRT expression in humans have been identified so far. In this study, we investigated the presence of DNA methylation in regulatory ...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Cejas RB,Ferguson DC,Quiñones-Lombraña A,Bard JE,Blanco JG

    更新日期:2019-06-17 00:00:00

  • Image-based closed-loop feedback for highly mono-dispersed microdroplet production.

    abstract::Micron-scale droplets isolated by an immiscible liquid can provide miniaturised reaction vessels which can be manipulated in microfluidic networks, and has seen a rapid growth in development. In many experiments, the precise volume of these microdroplets is a critical parameter which can be influenced by many external...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Crawford DF,Smith CA,Whyte G

    更新日期:2017-09-05 00:00:00

  • Inhibition of protein aggregation by zwitterionic polymer-based core-shell nanogels.

    abstract::Protein aggregation is a process by which misfolded proteins polymerizes into aggregates and forms fibrous structures with a β-sheet conformation, known as amyloids. It is an undesired outcome, as it not only causes numerous neurodegenerative diseases, but is also a major deterrent in the development of protein biopha...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Rajan R,Matsumura K

    更新日期:2017-04-04 00:00:00

  • Artificial targeting of misfolded cytosolic proteins to endoplasmic reticulum as a mechanism for clearance.

    abstract::We report that misfolded cytosolic proteins can be cleared from mammalian cells by directing them to endoplasmic reticulum (ER). NAT1 R64W and Parkin R42P are naturally occurring misfolded variants of cytosolic enzymes that acetylate arylamines and ubiquitinate proteins, respectively. We demonstrate that proteasome in...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Liu F,Koepp DM,Walters KJ

    更新日期:2015-07-14 00:00:00

  • Novel selective, potent naphthyl TRPM8 antagonists identified through a combined ligand- and structure-based virtual screening approach.

    abstract::Transient receptor potential melastatin 8 (TRPM8), a nonselective cation channel, is the predominant mammalian cold temperature thermosensor and it is activated by cold temperatures and cooling compounds, such as menthol and icilin. Because of its role in cold allodynia, cold hyperalgesia and painful syndromes TRPM8 a...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Beccari AR,Gemei M,Lo Monte M,Menegatti N,Fanton M,Pedretti A,Bovolenta S,Nucci C,Molteni A,Rossignoli A,Brandolini L,Taddei A,Za L,Liberati C,Vistoli G

    更新日期:2017-09-08 00:00:00

  • Spatially organizing biochemistry: choosing a strategy to translate synthetic biology to the factory.

    abstract::Natural biochemical systems are ubiquitously organized both in space and time. Engineering the spatial organization of biochemistry has emerged as a key theme of synthetic biology, with numerous technologies promising improved biosynthetic pathway performance. One strategy, however, may produce disparate results for d...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Jakobson CM,Tullman-Ercek D,Mangan NM

    更新日期:2018-05-29 00:00:00

  • Enhanced homologous recombination by the modulation of targeting vector ends.

    abstract::The field of genome editing was founded on the establishment of methods, such as the clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated protein (CRISPR/Cas) system, used to target DNA double-strand breaks (DSBs). However, the efficiency of genome editing also largely depends on the...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Hirotsune S,Kiyonari H,Jin M,Kumamoto K,Yoshida K,Shinohara M,Watanabe H,Wynshaw-Boris A,Matsuzaki F

    更新日期:2020-02-13 00:00:00

  • Electrically controllable sudden reversals in spin and valley polarization in silicene.

    abstract::We study the spin and valley dependent transport in a silicene superlattice under the influence of a magnetic exchange field, a perpendicular electric field and a voltage potential. It is found that a gate-voltage-controllable fully spin and valley polarized current can be obtained in the proposed device, and the spin...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Zhang Q,Chan KS,Li J

    更新日期:2016-09-20 00:00:00

  • Higher cytolytic score correlates with an immunosuppressive tumor microenvironment and reduced survival in glioblastoma.

    abstract::Cytolytic score (CYT), calculated from mRNA expression levels of granzyme and perforin, positively correlates with CD8+ T cell infiltration/activity in a variety of cancers. Unlike other cancers, higher CYT has been associated with worse prognosis in glioblastoma (GBM). To address this discrepancy, we sought to invest...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Haddad AF,Chen JS,Oh T,Pereira MP,Joshi RS,Aghi MK

    更新日期:2020-10-16 00:00:00

  • Injection of Fluoro-Gold into the tibial nerve leads to prolonged but reversible functional deficits in rats.

    abstract::Tract tracing with neuronal tracers not only represents a straightforward approach to identify axonal projection connection between regions of the nervous system at distance but also provides compelling evidence for axonal regeneration. An ideal neuronal tracer meets certain criteria including high labeling efficacy, ...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Mi D,Yuan Y,Zhang Y,Niu J,Wang Y,Yan J,Yang Y,Hu W

    更新日期:2019-07-09 00:00:00

  • Electronic Health Record Driven Prediction for Gestational Diabetes Mellitus in Early Pregnancy.

    abstract::Gestational diabetes mellitus (GDM) is conventionally confirmed with oral glucose tolerance test (OGTT) in 24 to 28 weeks of gestation, but it is still uncertain whether it can be predicted with secondary use of electronic health records (EHRs) in early pregnancy. To this purpose, the cost-sensitive hybrid model (CSHM...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Qiu H,Yu HY,Wang LY,Yao Q,Wu SN,Yin C,Fu B,Zhu XJ,Zhang YL,Xing Y,Deng J,Yang H,Lei SD

    更新日期:2017-11-27 00:00:00

  • Efficient Blood-Brain Barrier Opening in Primates with Neuronavigation-Guided Ultrasound and Real-Time Acoustic Mapping.

    abstract::Brain diseases including neurological disorders and tumors remain under treated due to the challenge to access the brain, and blood-brain barrier (BBB) restricting drug delivery which, also profoundly limits the development of pharmacological treatment. Focused ultrasound (FUS) with microbubbles is the sole method to ...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Wu SY,Aurup C,Sanchez CS,Grondin J,Zheng W,Kamimura H,Ferrera VP,Konofagou EE

    更新日期:2018-05-22 00:00:00

  • Rapamycin protects against gentamicin-induced acute kidney injury via autophagy in mini-pig models.

    abstract::Gentamicin may cause acute kidney injury. The pathogenesis of gentamicin nephrotoxicity is unclear. Autophagy is a highly conserved physiological process involved in removing damaged or aged biological macromolecules and organelles from the cytoplasm. The role of autophagy in the pathogenesis of gentamicin nephrotoxic...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Cui J,Bai XY,Sun X,Cai G,Hong Q,Ding R,Chen X

    更新日期:2015-06-08 00:00:00

  • Automated Retinal Layer Segmentation Using Graph-based Algorithm Incorporating Deep-learning-derived Information.

    abstract::Regular drusen, an accumulation of material below the retinal pigment epithelium (RPE), have long been established as a hallmark early feature of nonneovascular age-related macular degeneration (AMD). Advances in imaging have expanded the phenotype of AMD to include another extracellular deposit, reticular pseudodruse...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Mishra Z,Ganegoda A,Selicha J,Wang Z,Sadda SR,Hu Z

    更新日期:2020-06-12 00:00:00

  • High temporal resolution of leaf area data improves empirical estimation of grain yield.

    abstract::Empirical yield estimation from satellite data has long lacked suitable combinations of spatial and temporal resolutions. Consequently, the selection of metrics, i.e., temporal descriptors that predict grain yield, has likely been driven by practicality and data availability rather than by systematic targetting of cri...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Waldner F,Horan H,Chen Y,Hochman Z

    更新日期:2019-10-31 00:00:00

  • Synthesis and structure of a new thiazoline-based palladium(II) complex that promotes cytotoxicity and apoptosis of human promyelocytic leukemia HL-60 cells.

    abstract::Cisplatin is one of the most widely used chemotherapeutic agents in the treatment of different tumors but has high toxicity and side effects. Therefore, the synthesis of new chemotherapeutic agents is necessary, so that they are effective in the treatment of cancer while avoiding such toxicity. In this study, we have ...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Espino J,Fernández-Delgado E,Estirado S,de la Cruz-Martinez F,Villa-Carballar S,Viñuelas-Zahínos E,Luna-Giles F,Pariente JA

    更新日期:2020-10-07 00:00:00

  • Alcohol Consumption During Pregnancy and Risk of Placental Abnormality: The Japan Environment and Children's Study.

    abstract::There have been no large nationwide birth cohort studies examining for the effects of maternal alcohol use during pregnancy on placental abnormality. This study searched for associations between alcohol consumption and the placental abnormalities of placenta previa, placental abruption, and placenta accreta using the ...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Ohira S,Motoki N,Shibazaki T,Misawa Y,Inaba Y,Kanai M,Kurita H,Shiozawa T,Nakazawa Y,Tsukahara T,Nomiyama T,Japan Environment & Children’s Study (JECS) Group.

    更新日期:2019-07-16 00:00:00

  • Optical nonlinearity enhancement with graphene-decorated silicon waveguides.

    abstract::Broadband on-chip optical frequency combs (OFCs) are important for expanding the functionality of photonic integrated circuits. Here, we demonstrate a huge local optical nonlinearity enhancement using graphene. A waveguide is decorated with graphene by precisely manipulating graphene's area and position. Our approach ...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Ishizawa A,Kou R,Goto T,Tsuchizawa T,Matsuda N,Hitachi K,Nishikawa T,Yamada K,Sogawa T,Gotoh H

    更新日期:2017-04-12 00:00:00

  • Rigorous broadband study of the intrinsic ferromagnetic linewidth of monocrystalline garnet spheres.

    abstract::This work demonstrates the first application of direct broadband (1 GHz-30 GHz) quality (Q) factor measurements of the uniform precession mode in magnetised garnet spheres for the accurate determination of the room-temperature intrinsic ferromagnetic linewidth (ΔH). The spheres were enclosed in a subwavelength cavity,...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Pacewicz A,Krupka J,Salski B,Aleshkevych P,Kopyt P

    更新日期:2019-07-01 00:00:00

  • Towards a reliable assessment of Asian elephant population parameters: the application of photographic spatial capture-recapture sampling in a priority floodplain ecosystem.

    abstract::The hitherto difficult task of reliably estimating populations of wide-ranging megafauna has been enabled by advances in capture-recapture methodology. Here we combine photographic sampling with a Bayesian spatially-explicit capture-recapture (SCR) model to estimate population parameters for the endangered Asian eleph...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Goswami VR,Yadava MK,Vasudev D,Prasad PK,Sharma P,Jathanna D

    更新日期:2019-06-12 00:00:00

  • Genome-wide analysis of enhancer RNA in gene regulation across 12 mouse tissues.

    abstract::Enhancers play a crucial role in gene regulation but the participation of enhancer transcripts (i.e. enhancer RNA, eRNAs) in regulatory systems remains unclear. We provide a computational analysis on eRNAs using genome-wide data across 12 mouse tissues. The expression of genes targeted by transcribing enhancer is posi...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Cheng JH,Pan DZ,Tsai ZT,Tsai HK

    更新日期:2015-07-29 00:00:00