Elucidating a role for the cytoplasmic domain in the Mycobacterium tuberculosis mechanosensitive channel of large conductance.


:Microbial survival in dynamic environments requires the ability to successfully respond to abrupt changes in osmolarity. The mechanosensitive channel of large conductance (MscL) is a ubiquitous channel that facilitates the survival of bacteria and archaea under severe osmotic downshock conditions by relieving excess turgor pressure in response to increased membrane tension. A prominent structural feature of MscL, the cytoplasmic C-terminal domain, has been suggested to influence channel assembly and function. In this report, we describe the X-ray crystal structure and electrophysiological properties of a C-terminal domain truncation of the Mycobacterium tuberculosis MscL (MtMscLΔC). A crystal structure of MtMscLΔC solubilized in the detergent n-dodecyl-β-D-maltopyranoside reveals the pentameric, closed state-like architecture for the membrane spanning region observed in the previously solved full-length MtMscL. Electrophysiological characterization demonstrates that MtMscLΔC retains mechanosensitivity, but with conductance and tension sensitivity more closely resembling full length EcMscL than MtMscL. This study establishes that the C-terminal domain of MtMscL is not required for oligomerization of the full-length channel, but rather influences the tension sensitivity and conductance properties of the channel. The collective picture that emerges from these data is that each MscL channel structure has characteristic features, highlighting the importance of studying multiple homologs.


Sci Rep


Scientific reports


Herrera N,Maksaev G,Haswell ES,Rees DC




Has Abstract


2018-10-01 00:00:00












  • A Peptide-Nanoparticle System with Improved Efficacy against Multidrug Resistant Bacteria.

    abstract::The recent rise of multidrug resistant microbial strains requires development of new and novel therapeutic alternatives. In this study, we present a novel antibacterial system that comprises of modified naturally abundant antimicrobial peptides in conjugation with silver nanoparticles. Further, we propose a simple rou...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Pal I,Bhattacharyya D,Kar RK,Zarena D,Bhunia A,Atreya HS

    更新日期:2019-03-14 00:00:00

  • Non-linear Equation using Plasma Brain Natriuretic Peptide Levels to Predict Cardiovascular Outcomes in Patients with Heart Failure.

    abstract::Brain natriuretic peptide (BNP) is the most effective predictor of outcomes in chronic heart failure (CHF). This study sought to determine the qualitative relationship between the BNP levels at discharge and on the day of cardiovascular events in CHF patients. We devised a mathematical probabilistic model between the ...

    journal_title:Scientific reports

    pub_type: 临床试验,杂志文章


    authors: Fukuda H,Suwa H,Nakano A,Sakamoto M,Imazu M,Hasegawa T,Takahama H,Amaki M,Kanzaki H,Anzai T,Mochizuki N,Ishii A,Asanuma H,Asakura M,Washio T,Kitakaze M

    更新日期:2016-11-15 00:00:00

  • A randomized controlled trial of motor imagery combined with structured progressive circuit class therapy on gait in stroke survivors.

    abstract::Structured Progressive Circuit Class Therapy (SPCCT) was developed based on task-oriented therapy, providing benefits to patients' motivation and motor function. Training with Motor Imagery (MI) alone can improve gait performance in stroke survivors, but a greater effect may be observed when combined with SPCCT. Healt...

    journal_title:Scientific reports

    pub_type: 杂志文章,随机对照试验


    authors: Bovonsunthonchai S,Aung N,Hiengkaew V,Tretriluxana J

    更新日期:2020-04-24 00:00:00

  • Hydrogen-bonded host-guest systems are stable in ionic liquids.

    abstract::We show that H-bonded host-guest systems associate in ionic liquids (ILs), pure salts with melting point below room temperature, in which dipole-dipole electrostatic interactions should be negligible in comparison with dipole-charge interactions. Binding constants (Ka) obtained from titrations of four H-bonded host-gu...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Naranjo T,Álvarez-Asencio R,Pedraz P,Nieto-Ortega B,Silva SM,Burzurí E,Rutland MW,Pérez EM

    更新日期:2020-09-22 00:00:00

  • Effects of intramuscular and vaginal progesterone supplementation on frozen-thawed embryo transfer.

    abstract::This study was to investigate effects of progesterone vaginal sustained-release gel and intramuscular injection of progesterone on frozen-thawed embryos transfer in 3013 patients receiving vaginal progesterone sustained-release gel and progesterone injection in artificial cycle for frozen-thawed embryo transfer. All p...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Jiang L,Luo ZY,Hao GM,Gao BL

    更新日期:2019-10-24 00:00:00

  • Experimental demonstration of plasmon enhanced energy transfer rate in NaYF4:Yb(3+),Er(3+) upconversion nanoparticles.

    abstract::Energy transfer upconversion (ETU) is known to be the most efficient frequency upconversion mechanism. Surface plasmon can further enhance the upconversion process, opening doors to many applications. However, ETU is a complex process involving competing transitions between multiple energy levels and it has been diffi...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Lu D,Mao C,Cho SK,Ahn S,Park W

    更新日期:2016-01-07 00:00:00

  • Novel phages of healthy skin metaviromes from South Africa.

    abstract::Recent skin metagenomic studies have investigated the harbored viral diversity and its possible influence on healthy skin microbial populations, and tried to establish global patterns of skin-phage evolution. However, the detail associated with the phages that potentially play a role in skin health has not been invest...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: van Zyl LJ,Abrahams Y,Stander EA,Kirby-McCollough B,Jourdain R,Clavaud C,Breton L,Trindade M

    更新日期:2018-08-16 00:00:00

  • Method for high frequency tracking and sub-nm sample stabilization in single molecule fluorescence microscopy.

    abstract::While fluorescence microscopes and atomic force microscopes are widely used to visualize, track, and manipulate single biomolecules, the resolution of these methods is limited by sample drift. To minimize drift, active feedback methods have recently been used to stabilize single molecule microscopes on the sub-nanomet...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Schmidt PD,Reichert BH,Lajoie JG,Sivasankar S

    更新日期:2018-09-17 00:00:00

  • Obstructive Sleep Apnea and the Subsequent Risk of Chronic Rhinosinusitis: A Population-Based Study.

    abstract::The relationship between obstructive sleep apnea (OSA) and chronic rhinosinusitis (CRS) still remains unclear. This retrospective cohort study aimed to investigate the relationship between OSA and subsequent CRS using a population-based dataset. The study used data from the Taiwan Longitudinal Health Insurance Databas...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Kao LT,Hung SH,Lin HC,Liu CK,Huang HM,Wu CS

    更新日期:2016-02-10 00:00:00

  • Abstract images and words can convey the same meaning.

    abstract::Intuitively, deriving meaning from an abstract image is a uniquely human, idiosyncratic experience. Here we show that, despite having no universally recognised lexical association, abstract images spontaneously elicit specific concepts conveyed by words, with a consistency akin to that of concrete images. We presented...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Kuipers JR,Jones MW,Thierry G

    更新日期:2018-05-08 00:00:00

  • Amiodarone as an autophagy promoter reduces liver injury and enhances liver regeneration and survival in mice after partial hepatectomy.

    abstract::The deregulation of autophagy is involved in liver regeneration. Here, we investigated the role of autophagy in the regulation of liver regeneration after partial hepatectomy (PHx) and the development of pharmacological interventions for improved liver regeneration after PHx. We show that autophagy was activated in th...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Lin CW,Chen YS,Lin CC,Chen YJ,Lo GH,Lee PH,Kuo PL,Dai CY,Huang JF,Chung WL,Yu ML

    更新日期:2015-10-30 00:00:00

  • Characterization of a xylanase-producing Cellvibrio mixtus strain J3-8 and its genome analysis.

    abstract::Cellvibrio mixtus strain J3-8 is a gram-negative, xylanase-producing aerobic soil bacterium isolated from giant snails in Singapore. It is able to produce up to 10.1 U ml(-1) of xylanase, which is comparable to xylanase production from known bacterial and fungal strains. Genome sequence analysis of strain J3-8 reveals...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Wu YR,He J

    更新日期:2015-05-21 00:00:00

  • Design, transform and control of optical field in discrete optical system: an example.

    abstract::A discrete optical system can broaden the spatial distribution of the input light through optical coupling in array waveguides, just like diffraction in continuous media. Here, we theoretically demonstrate several kinds of control methods of optical field propagation in a discrete optical system, which is composed of ...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Deng H,Yuan Y,Yuan L

    更新日期:2017-07-12 00:00:00

  • Effect of acute exercise on RBC deformability and RBC nitric oxide synthase signalling pathway in young sickle cell anaemia patients.

    abstract::Sickle cell anaemia (SCA) is characterized by reduced red blood cell (RBC) deformability and nitric oxide (NO) bioavailability. The aim of the study was to investigate whether exercise might affect these parameters in SCA. SCA patients and healthy controls (AA) performed an acute submaximal exercise test until subject...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Grau M,Jerke M,Nader E,Schenk A,Renoux C,Collins B,Dietz T,Bizjak DA,Joly P,Bloch W,Connes P,Prokop A

    更新日期:2019-08-14 00:00:00

  • Proof-of-Concept Study of Drug Brain Permeability Between in Vivo Human Brain and an in Vitro iPSCs-Human Blood-Brain Barrier Model.

    abstract::The development of effective central nervous system (CNS) drugs has been hampered by the lack of robust strategies to mimic the blood-brain barrier (BBB) and cerebrovascular impairments in vitro. Recent technological advancements in BBB modeling using induced pluripotent stem cells (iPSCs) allowed to overcome some of ...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Roux GL,Jarray R,Guyot AC,Pavoni S,Costa N,Théodoro F,Nassor F,Pruvost A,Tournier N,Kiyan Y,Langer O,Yates F,Deslys JP,Mabondzo A

    更新日期:2019-11-05 00:00:00

  • Estimating and mitigating amplification bias in qualitative and quantitative arthropod metabarcoding.

    abstract::Amplicon based metabarcoding promises rapid and cost-efficient analyses of species composition. However, it is disputed whether abundance estimates can be derived from metabarcoding due to taxon specific PCR amplification biases. PCR-free approaches have been suggested to mitigate this problem, but come with considera...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Krehenwinkel H,Wolf M,Lim JY,Rominger AJ,Simison WB,Gillespie RG

    更新日期:2017-12-15 00:00:00

  • Universality of fragment shapes.

    abstract::The shape of fragments generated by the breakup of solids is central to a wide variety of problems ranging from the geomorphic evolution of boulders to the accumulation of space debris orbiting Earth. Although the statistics of the mass of fragments has been found to show a universal scaling behavior, the comprehensiv...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Domokos G,Kun F,Sipos AÁ,Szabó T

    更新日期:2015-03-16 00:00:00

  • Anisotropic Optical Response of Silver Nanorod Arrays: Surface Enhanced Raman Scattering Polarization and Angular Dependences Confronted with Ellipsometric Parameters.

    abstract::Silver nanorod arrays prepared by oblique angle deposition (AgOADs) represent versatile, simple and inexpensive substrates for high sensitivity surface enhanced Raman scattering (SERS) applications. Their anisotropic nature suggests that their optical responses such as the SERS signal, the depolarization ratio, reflec...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Šubr M,Petr M,Kylián O,Štěpánek J,Veis M,Procházka M

    更新日期:2017-06-27 00:00:00

  • Pulse-Wave-Pattern Classification with a Convolutional Neural Network.

    abstract::Owing to the diversity of pulse-wave morphology, pulse-based diagnosis is difficult, especially pulse-wave-pattern classification (PWPC). A powerful method for PWPC is a convolutional neural network (CNN). It outperforms conventional methods in pattern classification due to extracting informative abstraction and featu...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Li G,Watanabe K,Anzai H,Song X,Qiao A,Ohta M

    更新日期:2019-10-17 00:00:00

  • Mind wandering simultaneously prolongs reactions and promotes creative incubation.

    abstract::Mind wandering (MW) refers to the disengagement of attention from the external environment and the generation of thoughts unrelated to the task at hand. It is a ubiquitous cognitive process resulting in lapses of attention. MW imposes a negative impact on attention-based task performance, but also has been associated ...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Leszczynski M,Chaieb L,Reber TP,Derner M,Axmacher N,Fell J

    更新日期:2017-08-31 00:00:00

  • Active neutron and gamma-ray imaging of highly enriched uranium for treaty verification.

    abstract::The detection and characterization of highly enriched uranium (HEU) presents a large challenge in the non-proliferation field. HEU has a low neutron emission rate and most gamma rays are low energy and easily shielded. To address this challenge, an instrument known as the dual-particle imager (DPI) was used with a por...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Hamel MC,Polack JK,Ruch ML,Marcath MJ,Clarke SD,Pozzi SA

    更新日期:2017-08-11 00:00:00

  • Identification of novel molecular signatures of IgA nephropathy through an integrative -omics analysis.

    abstract::IgA nephropathy (IgAN) is the most prevalent among primary glomerular diseases worldwide. Although our understanding of IgAN has advanced significantly, its underlying biology and potential drug targets are still unexplored. We investigated a combinatorial approach for the analysis of IgAN-relevant -omics data, aiming...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Krochmal M,Cisek K,Filip S,Markoska K,Orange C,Zoidakis J,Gakiopoulou C,Spasovski G,Mischak H,Delles C,Vlahou A,Jankowski J

    更新日期:2017-08-22 00:00:00

  • Radiographic Knee Osteoarthritis and Knee Pain: Cross-sectional study from Five Different Racial/Ethnic Populations.

    abstract::The weak correlation between pain and structural changes in knee osteoarthritis is widely reported. In a previous within-person, knee-matched case-control study among Caucasians, the severity of radiographic osteoarthritis (ROA) was strongly associated with both the presence of frequent knee pain and pain severity. We...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Wang K,Kim HA,Felson DT,Xu L,Kim DH,Nevitt MC,Yoshimura N,Kawaguchi H,Lin J,Kang X,Zhang Y

    更新日期:2018-01-22 00:00:00

  • Root biomass and exudates link plant diversity with soil bacterial and fungal biomass.

    abstract::Plant diversity has been shown to determine the composition and functioning of soil biota. Although root-derived organic inputs are discussed as the main drivers of soil communities, experimental evidence is scarce. While there is some evidence that higher root biomass at high plant diversity increases substrate avail...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Eisenhauer N,Lanoue A,Strecker T,Scheu S,Steinauer K,Thakur MP,Mommer L

    更新日期:2017-04-04 00:00:00

  • A novel composite conductive microfiltration membrane and its anti-fouling performance with an external electric field in membrane bioreactors.

    abstract::Membrane fouling remains an obstacle to wide-spread applications of membrane bioreactors (MBRs) for wastewater treatment and reclamation. Herein, we report a simple method to prepare a composite conductive microfiltration (MF) membrane by introducing a stainless steel mesh into a polymeric MF membrane and to effective...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Huang J,Wang Z,Zhang J,Zhang X,Ma J,Wu Z

    更新日期:2015-03-18 00:00:00

  • Selective cognitive dysfunction and physical disability improvement after autologous hematopoietic stem cell transplantation in highly active multiple sclerosis.

    abstract::The aim was to assess the cognitive dysfunction and physical disability after autologous hematopoietic stem cell transplantation (AHSCT), to explore the potential factors influencing disability regression after AHSCT and to estimate the safety of low-dose immunosuppressive therapy in highly active Multiple Sclerosis (...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Giedraitiene N,Kizlaitiene R,Peceliunas V,Griskevicius L,Kaubrys G

    更新日期:2020-12-04 00:00:00

  • Artificial biofilms establish the role of matrix interactions in staphylococcal biofilm assembly and disassembly.

    abstract::We demonstrate that the microstructural and mechanical properties of bacterial biofilms can be created through colloidal self-assembly of cells and polymers, and thereby link the complex material properties of biofilms to well understood colloidal and polymeric behaviors. This finding is applied to soften and disassem...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Stewart EJ,Ganesan M,Younger JG,Solomon MJ

    更新日期:2015-08-14 00:00:00

  • Impact of HCV core gene quasispecies on hepatocellular carcinoma risk among HALT-C trial patients.

    abstract::Mutations at positions 70 and/or 91 in the core protein of genotype-1b, hepatitis C virus (HCV) are associated with hepatocellular carcinoma (HCC) risk in Asian patients. To evaluate this in a US population, the relationship between the percentage of 70 and/or 91 mutant HCV quasispecies in baseline serum samples of ch...

    journal_title:Scientific reports

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: El-Shamy A,Pendleton M,Eng FJ,Doyle EH,Bashir A,Branch AD

    更新日期:2016-06-01 00:00:00

  • Measuring Tumor Mutational Burden (TMB) in Plasma from mCRPC Patients Using Two Commercial NGS Assays.

    abstract::Tumor tissue mutational burden (TMB) has emerged as a promising predictive biomarker for immune checkpoint therapy. Measuring TMB from circulating tumor DNA (ctDNA) found in plasma is attractive in tissue-constrained indications. We compared the performance of two plasma-based commercial TMB assays including the effec...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Qiu P,Poehlein CH,Marton MJ,Laterza OF,Levitan D

    更新日期:2019-01-14 00:00:00

  • Hollow porous SiO2 nanocubes towards high-performance anodes for lithium-ion batteries.

    abstract::The high theoretical capacity and low discharge potential of silicon have attracted much attention on Si-based anodes. Herein, hollow porous SiO2 nanocubes have been prepared via a two-step hard-template process and evaluated as electrode materials for lithium-ion batteries. The hollow porous SiO2 nanocubes exhibited ...

    journal_title:Scientific reports

    pub_type: 杂志文章


    authors: Yan N,Wang F,Zhong H,Li Y,Wang Y,Hu L,Chen Q

    更新日期:2013-01-01 00:00:00