Oestradiol induction of a glucocorticoid-responsive gene by a chimaeric receptor.


:Steroid hormone receptors are a class of cell-specific trans-acting transcription regulatory factors whose activity is controlled by specific binding of the hormone. The hormone-receptor complex appears to associate with promoter/enhancer elements of specific target genes, resulting in activation of transcription (see refs 1 and 2 for reviews). Sequence comparison between the oestrogen, glucocorticoid and progesterone receptors (refs 7, 8 and unpublished results) and site-directed mutation analysis, has identified in each at least two functional domains important for steroid receptor function. Region E (Fig. 1a), is the hormone-binding domain; region C is a 66-amino-acid region (Figs 1a,b) that is more highly conserved than the hormone-binding domain and has the potential to form at least two zinc-stabilized 'DNA-binding fingers' analogous to those proposed for the Xenopus transcription factor TFIIIA. We and others have suggested that this region may be the receptor's DNA-binding domain. We show here that point mutations replacing two cysteines by two histidines in the first potential DNA-binding finger of the human oestrogen receptor (hER) prevent it from activating gene transcription. We further show that a chimaeric receptor formed by replacing this 66-amino-acid region of the hER with that of the human glucocorticoid receptor (hGR) activates expression of a glucocorticoid-inducible gene, but not of an oestrogen-inducible gene, in the presence of oestradiol. Thus, region C determines the receptor's specificity for target genes.






Green S,Chambon P




Has Abstract


1987-01-01 00:00:00














  • The genome sequence of Schizosaccharomyces pombe.

    abstract::We have sequenced and annotated the genome of fission yeast (Schizosaccharomyces pombe), which contains the smallest number of protein-coding genes yet recorded for a eukaryote: 4,824. The centromeres are between 35 and 110 kilobases (kb) and contain related repeats including a highly conserved 1.8-kb element. Regions...


    pub_type: 杂志文章


    authors: Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fr

    更新日期:2002-02-21 00:00:00

  • Paths to understanding the genetic basis of autoimmune disease.

    abstract::Some people inherit an unfortunate combination of genetic sequences, such that exposure to an external trigger causes their immune response to turn on their own tissues. Although mutations in a single gene can cause autoimmunity, most autoimmune diseases are associated with several sequence variants. Marked advances i...


    pub_type: 杂志文章,评审


    authors: Rioux JD,Abbas AK

    更新日期:2005-06-02 00:00:00

  • A primitive placoderm sheds light on the origin of the jawed vertebrate face.

    abstract::Extant vertebrates form two clades, the jawless Cyclostomata (lampreys and hagfishes) and the jawed Gnathostomata (all other vertebrates), with contrasting facial architectures. These arise during development from just a few key differences in the growth patterns of the cranial primordia: notably, the nasal sacs and h...


    pub_type: 杂志文章


    authors: Dupret V,Sanchez S,Goujet D,Tafforeau P,Ahlberg PE

    更新日期:2014-03-27 00:00:00

  • Crystal structure of the specificity domain of ribonuclease P.

    abstract::RNase P is the only endonuclease responsible for processing the 5' end of transfer RNA by cleaving a precursor and leading to tRNA maturation. It contains an RNA component and a protein component and has been identified in all organisms. It was one of the first catalytic RNAs identified and the first that acts as a mu...


    pub_type: 杂志文章


    authors: Krasilnikov AS,Yang X,Pan T,Mondragón A

    更新日期:2003-02-13 00:00:00

  • Author Correction: SLC19A1 transports immunoreactive cyclic dinucleotides.

    abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...


    pub_type: 杂志文章,已发布勘误


    authors: Luteijn RD,Zaver SA,Gowen BG,Wyman SK,Garelis NE,Onia L,McWhirter SM,Katibah GE,Corn JE,Woodward JJ,Raulet DH

    更新日期:2020-03-01 00:00:00

  • Structural details of an adenine tract that does not cause DNA to bend.

    abstract::Runs of adenines (adenine tracts) have been implicated as the main determinant of sequence-directed DNA bending. The most widely used experimental test for bending relies on the observation that bent DNA migrates more slowly than straight DNA on a polyacrylamide electrophoresis gel. It was shown recently that the poly...


    pub_type: 杂志文章


    authors: Burkhoff AM,Tullius TD

    更新日期:1988-02-04 00:00:00

  • Regulation of embryonic growth and lysosomal targeting by the imprinted Igf2/Mpr gene.

    abstract::The receptor for insulin-like growth factor type 2, also known as the cation-independent mannose-6-phosphate receptor (Igf2/Mpr), is a multifunctional receptor thought to play a role in lysosomal targeting, cell growth and signal transduction. Igf2/Mpr has been mapped to the mouse Tme locus and shown to be an imprinte...


    pub_type: 杂志文章


    authors: Wang ZQ,Fung MR,Barlow DP,Wagner EF

    更新日期:1994-12-01 00:00:00

  • Direct observation of kinesin stepping by optical trapping interferometry.

    abstract::Do biological motors move with regular steps? To address this question, we constructed instrumentation with the spatial and temporal sensitivity to resolve movement on a molecular scale. We deposited silica beads carrying single molecules of the motor protein kinesin on microtubules using optical tweezers and analysed...


    pub_type: 杂志文章


    authors: Svoboda K,Schmidt CF,Schnapp BJ,Block SM

    更新日期:1993-10-21 00:00:00

  • Towards complete cofactor arrangement in the 3.0 A resolution structure of photosystem II.

    abstract::Oxygenic photosynthesis in plants, algae and cyanobacteria is initiated at photosystem II, a homodimeric multisubunit protein-cofactor complex embedded in the thylakoid membrane. Photosystem II captures sunlight and powers the unique photo-induced oxidation of water to atmospheric oxygen. Crystallographic investigatio...


    pub_type: 杂志文章


    authors: Loll B,Kern J,Saenger W,Zouni A,Biesiadka J

    更新日期:2005-12-15 00:00:00

  • Crystal structure of nucleotide-free dynamin.

    abstract::Dynamin is a mechanochemical GTPase that oligomerizes around the neck of clathrin-coated pits and catalyses vesicle scission in a GTP-hydrolysis-dependent manner. The molecular details of oligomerization and the mechanism of the mechanochemical coupling are currently unknown. Here we present the crystal structure of h...


    pub_type: 杂志文章


    authors: Faelber K,Posor Y,Gao S,Held M,Roske Y,Schulze D,Haucke V,Noé F,Daumke O

    更新日期:2011-09-18 00:00:00

  • Synthesis of growth hormone by bacteria.

    abstract::A hybrid gene was constructed between the beta-lactamase gene of plasmid pBR322 and the cloned coding sequence for rat growth hormone. This gene is expressed in bacteria and growth hormone sequences are detectable by immunological methods. ...


    pub_type: 杂志文章


    authors: Seeburg PH,Shine J,Martial JA,Ivarie RD,Morris JA,Ullrich A,Baxter JD,Goodman HM

    更新日期:1978-12-21 00:00:00

  • A GPR174-CCL21 module imparts sexual dimorphism to humoral immunity.

    abstract::Humoral immune responses to immunization and infection and susceptibilities to antibody-mediated autoimmunity are generally lower in males1-3. However, the mechanisms underlying such sexual dimorphism are not well understood. Here we show that there are intrinsic differences between the B cells that produce germinal c...


    pub_type: 杂志文章


    authors: Zhao R,Chen X,Ma W,Zhang J,Guo J,Zhong X,Yao J,Sun J,Rubinfien J,Zhou X,Wang J,Qi H

    更新日期:2020-01-01 00:00:00

  • Photoelectrochemical cells.

    abstract::Until now, photovoltaics--the conversion of sunlight to electrical power--has been dominated by solid-state junction devices, often made of silicon. But this dominance is now being challenged by the emergence of a new generation of photovoltaic cells, based, for example, on nanocrystalline materials and conducting pol...


    pub_type: 杂志文章


    authors: Grätzel M

    更新日期:2001-11-15 00:00:00

  • Modes of faulting at mid-ocean ridges.

    abstract::Abyssal-hill-bounding faults that pervade the oceanic crust are the most common tectonic feature on the surface of the Earth. The recognition that these faults form at plate spreading centres came with the plate tectonic revolution. Recent observations reveal a large range of fault sizes and orientations; numerical mo...


    pub_type: 杂志文章


    authors: Buck WR,Lavier LL,Poliakov AN

    更新日期:2005-04-07 00:00:00

  • Natural and anthropogenic variations in methane sources during the past two millennia.

    abstract::Methane is an important greenhouse gas that is emitted from multiple natural and anthropogenic sources. Atmospheric methane concentrations have varied on a number of timescales in the past, but what has caused these variations is not always well understood. The different sources and sinks of methane have specific isot...


    pub_type: 历史文章,杂志文章


    authors: Sapart CJ,Monteil G,Prokopiou M,van de Wal RS,Kaplan JO,Sperlich P,Krumhardt KM,van der Veen C,Houweling S,Krol MC,Blunier T,Sowers T,Martinerie P,Witrant E,Dahl-Jensen D,Röckmann T

    更新日期:2012-10-04 00:00:00

  • Maternal mRNA deadenylation and decay by the piRNA pathway in the early Drosophila embryo.

    abstract::Piwi-associated RNAs (piRNAs), a specific class of 24- to 30-nucleotide-long RNAs produced by the Piwi-type of Argonaute proteins, have a specific germline function in repressing transposable elements. This repression is thought to involve heterochromatin formation and transcriptional and post-transcriptional silencin...


    pub_type: 杂志文章


    authors: Rouget C,Papin C,Boureux A,Meunier AC,Franco B,Robine N,Lai EC,Pelisson A,Simonelig M

    更新日期:2010-10-28 00:00:00

  • Human gut Bacteroidetes can utilize yeast mannan through a selfish mechanism.

    abstract::Yeasts, which have been a component of the human diet for at least 7,000 years, possess an elaborate cell wall α-mannan. The influence of yeast mannan on the ecology of the human microbiota is unknown. Here we show that yeast α-mannan is a viable food source for the Gram-negative bacterium Bacteroides thetaiotaomicron...


    pub_type: 杂志文章


    authors: Cuskin F,Lowe EC,Temple MJ,Zhu Y,Cameron E,Pudlo NA,Porter NT,Urs K,Thompson AJ,Cartmell A,Rogowski A,Hamilton BS,Chen R,Tolbert TJ,Piens K,Bracke D,Vervecken W,Hakki Z,Speciale G,Munōz-Munōz JL,Day A,Peña MJ,

    更新日期:2015-01-08 00:00:00

  • Publisher Correction: Deterministic delivery of remote entanglement on a quantum network.

    abstract::Change history: In this Letter, the received date should be 20 December 2017, instead of 27 April 2018. This has been corrected online. ...


    pub_type: 杂志文章,已发布勘误


    authors: Humphreys PC,Kalb N,Morits JPJ,Schouten RN,Vermeulen RFL,Twitchen DJ,Markham M,Hanson R

    更新日期:2018-10-01 00:00:00

  • Encoding of danger by parabrachial CGRP neurons.

    abstract::Animals must respond to various threats to survive. Neurons that express calcitonin gene-related peptide in the parabrachial nucleus (CGRPPBN neurons) relay sensory signals that contribute to satiation and pain-induced fear behaviour, but it is unclear how they encode these distinct processes. Here, by recording calci...


    pub_type: 杂志文章


    authors: Campos CA,Bowen AJ,Roman CW,Palmiter RD

    更新日期:2018-03-29 00:00:00

  • Bacterial pathogenomics.

    abstract::Genomes from all of the crucial bacterial pathogens of humans, plants and animals have now been sequenced, as have genomes from many of the important commensal, symbiotic and environmental microorganisms. Analysis of these sequences has revealed the forces that shape pathogen evolution and has brought to light unexpec...


    pub_type: 杂志文章,评审


    authors: Pallen MJ,Wren BW

    更新日期:2007-10-18 00:00:00

  • Rise of the temporary workforce.

    abstract:: ...


    pub_type: 评论,新闻


    authors: Gewin V

    更新日期:2019-02-01 00:00:00

  • Altered substrate specificity of herpes simplex virus thymidine kinase confers acyclovir-resistance.

    abstract::Acyclovir (9-[2-hydroxyethoxymethyl]guanine or ACV) is a nucleoside analogue with considerable potential for the treatment of herpes simplex virus (HSV) infections in man. Two virus-coded enzymes are important in the mechanism of action of this drug: thymidine kinase (TK) which initiates its activation by converting i...


    pub_type: 杂志文章


    authors: Darby G,Field HJ,Salisbury SA

    更新日期:1981-01-01 00:00:00

  • Ice-binding structure and mechanism of an antifreeze protein from winter flounder.

    abstract::Antifreeze proteins provide fish with protection against the freezing effect of polar environments by binding to ice surfaces and inhibiting growth of ice crystals. We present the X-ray crystal structure at 1.5 A resolution of a lone alpha-helical antifreeze protein from winter flounder, which provides a detailed look...


    pub_type: 杂志文章


    authors: Sicheri F,Yang DS

    更新日期:1995-06-01 00:00:00

  • MHC class II region encoding proteins related to the multidrug resistance family of transmembrane transporters.

    abstract::The T-cell immune response is directed against antigenic peptide fragments generated in intracellular compartments, the cytosol or the endocytic system. Peptides derived from cytosolic proteins, usually of biosynthetic origin, are presented efficiently to T-cell receptors by major histocompatibility complex (MHC) clas...


    pub_type: 杂志文章


    authors: Deverson EV,Gow IR,Coadwell WJ,Monaco JJ,Butcher GW,Howard JC

    更新日期:1990-12-20 00:00:00

  • An ultra-lightweight design for imperceptible plastic electronics.

    abstract::Electronic devices have advanced from their heavy, bulky origins to become smart, mobile appliances. Nevertheless, they remain rigid, which precludes their intimate integration into everyday life. Flexible, textile and stretchable electronics are emerging research areas and may yield mainstream technologies. Rollable ...


    pub_type: 杂志文章


    authors: Kaltenbrunner M,Sekitani T,Reeder J,Yokota T,Kuribara K,Tokuhara T,Drack M,Schwödiauer R,Graz I,Bauer-Gogonea S,Bauer S,Someya T

    更新日期:2013-07-25 00:00:00

  • Widespread changes in protein synthesis induced by microRNAs.

    abstract::Animal microRNAs (miRNAs) regulate gene expression by inhibiting translation and/or by inducing degradation of target messenger RNAs. It is unknown how much translational control is exerted by miRNAs on a genome-wide scale. We used a new proteomic approach to measure changes in synthesis of several thousand proteins i...


    pub_type: 杂志文章


    authors: Selbach M,Schwanhäusser B,Thierfelder N,Fang Z,Khanin R,Rajewsky N

    更新日期:2008-09-04 00:00:00

  • An unexpected specialization for horizontal disparity in primate primary visual cortex.

    abstract::The horizontal separation of the eyes means that objects nearer or farther than the fixation point project to different locations on the two retinae, differing principally in their horizontal coordinates (horizontal binocular disparity). Disparity-selective neurons have generally been studied with disparities applied ...


    pub_type: 杂志文章


    authors: Cumming BG

    更新日期:2002-08-08 00:00:00

  • A Crohn's disease variant in Atg16l1 enhances its degradation by caspase 3.

    abstract::Crohn's disease is a debilitating inflammatory bowel disease (IBD) that can involve the entire digestive tract. A single-nucleotide polymorphism (SNP) encoding a missense variant in the autophagy gene ATG16L1 (rs2241880, Thr300Ala) is strongly associated with the incidence of Crohn's disease. Numerous studies have dem...


    pub_type: 杂志文章


    authors: Murthy A,Li Y,Peng I,Reichelt M,Katakam AK,Noubade R,Roose-Girma M,DeVoss J,Diehl L,Graham RR,van Lookeren Campagne M

    更新日期:2014-02-27 00:00:00

  • Building C(sp3)-rich complexity by combining cycloaddition and C-C cross-coupling reactions.

    abstract::Prized for their ability to rapidly generate chemical complexity by building new ring systems and stereocentres1, cycloaddition reactions have featured in numerous total syntheses2 and are a key component in the education of chemistry students3. Similarly, carbon-carbon (C-C) cross-coupling methods are integral to syn...


    pub_type: 杂志文章


    authors: Chen TG,Barton LM,Lin Y,Tsien J,Kossler D,Bastida I,Asai S,Bi C,Chen JS,Shan M,Fang H,Fang FG,Choi HW,Hawkins L,Qin T,Baran PS

    更新日期:2018-08-01 00:00:00

  • Cranial skeletal biology.

    abstract::To artists, the face is a mirror of the soul. To biologists, the face reflects remarkable structural diversity--think of bulldogs and wolfhounds or galapagos finches. How do such variations in skeletal form arise? Do the same mechanisms control skeletogenesis elsewhere in the body? The answers lie in the molecular mac...


    pub_type: 杂志文章,评审


    authors: Helms JA,Schneider RA

    更新日期:2003-05-15 00:00:00