Oestradiol induction of a glucocorticoid-responsive gene by a chimaeric receptor.


:Steroid hormone receptors are a class of cell-specific trans-acting transcription regulatory factors whose activity is controlled by specific binding of the hormone. The hormone-receptor complex appears to associate with promoter/enhancer elements of specific target genes, resulting in activation of transcription (see refs 1 and 2 for reviews). Sequence comparison between the oestrogen, glucocorticoid and progesterone receptors (refs 7, 8 and unpublished results) and site-directed mutation analysis, has identified in each at least two functional domains important for steroid receptor function. Region E (Fig. 1a), is the hormone-binding domain; region C is a 66-amino-acid region (Figs 1a,b) that is more highly conserved than the hormone-binding domain and has the potential to form at least two zinc-stabilized 'DNA-binding fingers' analogous to those proposed for the Xenopus transcription factor TFIIIA. We and others have suggested that this region may be the receptor's DNA-binding domain. We show here that point mutations replacing two cysteines by two histidines in the first potential DNA-binding finger of the human oestrogen receptor (hER) prevent it from activating gene transcription. We further show that a chimaeric receptor formed by replacing this 66-amino-acid region of the hER with that of the human glucocorticoid receptor (hGR) activates expression of a glucocorticoid-inducible gene, but not of an oestrogen-inducible gene, in the presence of oestradiol. Thus, region C determines the receptor's specificity for target genes.






Green S,Chambon P




Has Abstract


1987-01-01 00:00:00














  • Sex in flies: the splice of life.

    abstract::The discovery that the primary transcripts of many genes are processed to produce more than one kind of messenger RNA focuses attention on the control of RNA processing as a developmental regulatory mechanism. In the fruit-fly Drosophila melanogaster, differential splicing of a hierarchy of regulatory genes determines...


    pub_type: 杂志文章,评审


    authors: Baker BS

    更新日期:1989-08-17 00:00:00

  • Conservation and change in the DNA sequences coding for alcohol dehydrogenase in sibling species of Drosophila.

    abstract::The DNA sequences of the alcohol dehydrogenase (Adh) genes of four very closely related species of Drosophila show that the rates of nucleotide change vary greatly in different functional domains of this gene. A phylogeny of these species based on the Adh sequence data is consistent with that based on polytene chromos...


    pub_type: 杂志文章


    authors: Bodmer M,Ashburner M

    更新日期:1984-05-31 00:00:00

  • Coexistence of Fermi arcs and Fermi pockets in a high-T(c) copper oxide superconductor.

    abstract::In the pseudogap state of the high-transition-temperature (high-T(c)) copper oxide superconductors, angle-resolved photoemission (ARPES) measurements have seen Fermi arcs-that is, open-ended gapless sections in the large Fermi surface-rather than a closed loop expected of an ordinary metal. This is all the more puzzli...


    pub_type: 杂志文章


    authors: Meng J,Liu G,Zhang W,Zhao L,Liu H,Jia X,Mu D,Liu S,Dong X,Zhang J,Lu W,Wang G,Zhou Y,Zhu Y,Wang X,Xu Z,Chen C,Zhou XJ

    更新日期:2009-11-19 00:00:00

  • Aggression by polyembryonic wasp soldiers correlates with kinship but not resource competition.

    abstract::Kin selection theory predicts that individuals will show less aggression and more altruism towards relatives. However, recent theoretical developments suggest that with limited dispersal, competition between relatives can override the effects of relatedness. The predicted and opposing influences of relatedness and com...


    pub_type: 杂志文章


    authors: Giron D,Dunn DW,Hardy IC,Strand MR

    更新日期:2004-08-05 00:00:00

  • Quantifying entanglement in macroscopic systems.

    abstract::Traditionally, entanglement was considered to be a quirk of microscopic objects that defied a common-sense explanation. Now, however, entanglement is recognized to be ubiquitous and robust. With the realization that entanglement can occur in macroscopic systems - and with the development of experiments aimed at exploi...


    pub_type: 杂志文章


    authors: Vedral V

    更新日期:2008-06-19 00:00:00

  • Localized sex in bacteria.

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    pub_type: 杂志文章


    authors: Smith JM,Dowson CG,Spratt BG

    更新日期:1991-01-03 00:00:00

  • Perovskite oxides for visible-light-absorbing ferroelectric and photovoltaic materials.

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    pub_type: 杂志文章


    authors: Grinberg I,West DV,Torres M,Gou G,Stein DM,Wu L,Chen G,Gallo EM,Akbashev AR,Davies PK,Spanier JE,Rappe AM

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  • Repeated tricyclics induce a progressive dopamine autoreceptor subsensitivity independent of daily drug treatment.

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    authors: Chiodo LA,Antelman SM

    更新日期:1980-10-02 00:00:00

  • A salmonella protein antagonizes Rac-1 and Cdc42 to mediate host-cell recovery after bacterial invasion.

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    authors: Fu Y,Galán JE

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    pub_type: 杂志文章,评审


    authors: Kersten S,Desvergne B,Wahli W

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    authors: Priya R,Allanki S,Gentile A,Mansingh S,Uribe V,Maischein HM,Stainier DYR

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    authors: Irwin MJ,Hudson KR,Fraser JD,Gascoigne NR

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    authors: Hetz SK,Bradley TJ

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    pub_type: 杂志文章


    authors: Cheour M,Martynova O,Näätänen R,Erkkola R,Sillanpää M,Kero P,Raz A,Kaipio ML,Hiltunen J,Aaltonen O,Savela J,Hämäläinen H

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    authors: Dörr JR,Yu Y,Milanovic M,Beuster G,Zasada C,Däbritz JH,Lisec J,Lenze D,Gerhardt A,Schleicher K,Kratzat S,Purfürst B,Walenta S,Mueller-Klieser W,Gräler M,Hummel M,Keller U,Buck AK,Dörken B,Willmitzer L,Reimann M,

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    authors: Lima CD,Wang JC,Mondragón A

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    authors: Stollewerk A,Schoppmeier M,Damen WG

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    pub_type: 杂志文章


    authors: Rothschild L

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    pub_type: 杂志文章,评审


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    authors: Png KJ,Halberg N,Yoshida M,Tavazoie SF

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    authors: Wang Y,Gao W,Shi X,Ding J,Liu W,He H,Wang K,Shao F

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    pub_type: 评论,杂志文章


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    pub_type: 杂志文章


    authors: Caroni P,Carlson SS,Schweitzer E,Kelly RB

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    pub_type: 杂志文章


    authors: Inada N,Oguri M,Pindor B,Hennawi JF,Chiu K,Zheng W,Ichikawa S,Gregg MD,Becker RH,Suto Y,Strauss MA,Turner EL,Keeton CR,Annis J,Castander FJ,Eisenstein DJ,Frieman JA,Fukugita M,Gunn JE,Johnston DE,Kent SM,Nichol

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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Rotzler S,Schramek H,Brenner HR

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