Abstract:
:There are currently no licensed therapeutic treatment or preventive vaccines against Ebolavirus disease, and the 2013-2016 West African outbreak of Ebolavirus disease spread rapidly and resulted in almost 30,000 cases and more than 11,000 deaths. However, the devastating outbreak has spurred the development of novel Ebolavirus vaccines. Here, we demonstrate that alphavirus-based DNA-launched self-replicating RNA replicon vaccines (DREP) encoding either the glycoprotein (GP) gene or co-expressing the GP and VP40 genes of Sudan or Zaire Ebolavirus are immunogenic in mice inducing both binding and neutralizing antibodies as well as CD8 T cell responses. In addition, antibodies were cross-reactive against another Ebolavirus, although the specificity was higher for the vaccination antigen. DREP vaccines were more immunogenic than recombinant MVA vaccines expressing the same Ebolavirus antigens. However, a DREP prime followed by an MVA boost immunization regimen improved vaccine immunogenicity as compared to DREP and MVA homologous prime-boost immunizations. Moreover, we show that a bivalent approach targeting both Sudan and Zaire Ebolavirus can be employed without significant loss of immunity. This opens for further investigation of a pan-Ebolavirus or even a pan-filovirus vaccine.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Öhlund P,García-Arriaza J,Zusinaite E,Szurgot I,Männik A,Kraus A,Ustav M,Merits A,Esteban M,Liljeström P,Ljungberg Kdoi
10.1038/s41598-018-31003-6subject
Has Abstractpub_date
2018-08-20 00:00:00pages
12459issue
1issn
2045-2322pii
10.1038/s41598-018-31003-6journal_volume
8pub_type
杂志文章abstract::Traumatic brain injury (TBI) has been associated with atherosclerosis and cardiovascular mortality in humans. However the causal relationship between TBI and vascular disease is unclear. This study investigated the direct role of TBI on vascular disease using a murine model of atherosclerosis. Apolipoprotein E deficie...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-23959-2
更新日期:2018-04-04 00:00:00
abstract::The cartilaginous and non-neopterygian bony fishes have an electric sense typically comprised of hundreds or thousands of sensory canals distributed in broad clusters over the head. This morphology facilitates neural encoding of local electric field intensity, orientation, and polarity, used for determining the positi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep08962
更新日期:2015-03-11 00:00:00
abstract::We propose a novel method to achieve tunable plasmon focusing in graphene/photonic-crystal hybrid structure exhibiting all-angle negative refraction at terahertz frequencies. A two-dimensional photonic crystal composed of a square lattice of dielectric rods is constructed on the substrate of a graphene sheet to provid...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep41788
更新日期:2017-02-02 00:00:00
abstract::β-lactam antibiotics act as suicide substrates of transpeptidases responsible for the last cross-linking step of peptidoglycan synthesis in the bacterial cell wall. Nucleophilic attack of the β-lactam carbonyl by the catalytic residue (Ser or Cys) of transpeptidases results in the opening of the β-lactam ring and in t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09341-8
更新日期:2017-08-22 00:00:00
abstract::This study evaluates the association between indoor microbial diversity early in life and hyperactivity/inattention symptoms in children at ages 10 and 15 years.A random sample enriched with subjects with hyperactivity/inattention at age 15 years was selected from the German LISA birth cohort. Bedroom floor dust was c...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-53527-1
更新日期:2019-11-22 00:00:00
abstract::Multiferroic BiFeO3 exhibits excellent magnetoelectric coupling critical for magnetic information processing with minimal power consumption. However, the degenerate nature of the easy spin axis in the (111) plane presents roadblocks for real world applications. Here, we explore the stabilization and switchability of t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep12969
更新日期:2015-08-06 00:00:00
abstract::Childhood obesity is a risk factor for asthma, but the molecular mechanisms linking both remain elusive. Since obesity leads to chronic low-grade inflammation and affects metabolic signaling we hypothesized that postnatal hyperalimentation (pHA) induced by maternal high-fat-diet during lactation leads to early-onset o...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep24168
更新日期:2016-04-18 00:00:00
abstract::Nerve fibers are known to reside within malignant tumors and the greater the neuronal density the worse prognosis for the patient. Recent discoveries using tumor bearing animal models have eluded to the autonomic nervous system having a direct effect on tumor growth and metastasis. We report the first direct and chron...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-71670-y
更新日期:2020-09-09 00:00:00
abstract::The relationships between polypeptide composition, sequence, structure and function have been puzzling biologists ever since first protein sequences were determined. Here, we study the statistics of occurrence of all possible pentapeptide sequences in known proteins. To compensate for the non-uniform distribution of i...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-33433-8
更新日期:2018-10-11 00:00:00
abstract::Type 2 diabetes mellitus (T2DM) is a low-grade chronic inflammatory diseases, which have been implicated in the pathogenesis of cognitive decline. We aim to evaluate associations between inflammatory markers and the risk of mild cognitive impairment (MCI) in T2DM. This study of 140 diabetic patients involved 71 with M...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-12515-z
更新日期:2017-09-26 00:00:00
abstract::The fly Ormia ochracea has the remarkable ability, given an eardrum separation of only 520 μm, to pinpoint the 5 kHz chirp of its cricket host. Previous research showed that the two eardrums are mechanically coupled, which amplifies the directional cues. We have now performed a mechanics and optimization analysis whic...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep02489
更新日期:2013-01-01 00:00:00
abstract::Optical thermometry has been widely studied to achieve an inaccessible temperature measurement in submicron scale and it has been reported that the temperature sensitivity depends mainly on host types. In this work, we propose a new method to improve the optical temperature sensitivity of Yb3+-Er3+ co-doped CaWO4 phos...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep43383
更新日期:2017-02-27 00:00:00
abstract::We employed an RNA-guided CRISPR/Cas9 DNA editing system to precisely remove the entire HIV-1 genome spanning between 5' and 3' LTRs of integrated HIV-1 proviral DNA copies from latently infected human CD4+ T-cells. Comprehensive assessment of whole-genome sequencing of HIV-1 eradicated cells ruled out any off-target ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep22555
更新日期:2016-03-04 00:00:00
abstract::To determine levels of change in risk factors for cardiovascular disease among people with and without a previous diagnosis of diabetes from 2007 to 2016 in Iran. Data were obtained from five rounds of the World Health Organization STEPwise approach to Surveillance (STEPS) cross-sectional surveys. Participants were 76...
journal_title:Scientific reports
pub_type: 历史文章,杂志文章
doi:10.1038/s41598-020-68640-9
更新日期:2020-07-16 00:00:00
abstract::Blood coagulation is regulated through protein-protein and protein-lipid interactions that occur at the sub-endothelium following vascular damage. Soluble clotting proteins bind to membrane components in a phosphatidylserine (PS) dependent manner to assemble multi-protein complexes that regulate clot formation; howeve...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-73647-3
更新日期:2020-10-15 00:00:00
abstract::The objective of this study was to assess the diagnosis value of urinary inflammatory index (UII) and systemic immune-inflammation index (SII) for UTI. Nine inflammatory indexes including neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio, SII and six UIIs were calculated for Receiver operating characteristi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-76352-3
更新日期:2020-11-09 00:00:00
abstract::Huntington disease (HD) is a late-onset genetic neurodegenerative disorder caused by expansion of cytosine-adenine-guanine (CAG) trinucleotide in the exon 1 of the gene encoding the polyglutamine (polyQ). It has been shown that protein degradation and lipid metabolism is altered in HD. In many neurodegenerative disord...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-19160-0
更新日期:2018-01-15 00:00:00
abstract::Preventing ischemia-related cell damage is a priority when preserving tissue for transplantation. Perfusion protocols have been established for a variety of applications and proven to be superior to procedures used in clinical routine. Extracorporeal perfusion of muscle tissue though cumbersome is highly desirable sin...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11956
更新日期:2015-07-06 00:00:00
abstract::We present measurements of the outdoor stability of PCDTBT:PC71BM based bulk heterojunction organic solar cells for over the course of a year. We find that the devices undergo a burn-in process lasting 450 hours followed by a TS80 lifetime of up to 6200 hours. We conclude that in the most stable devices, the observed ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep21632
更新日期:2016-02-09 00:00:00
abstract::Consistent cell preparation is a fundamental preliminary step for understanding complex cellular mechanisms in various cell-based research fields, including basic cell biology, cancer research, and tissue engineering. However, certain elusive factors, such as cellular de-differentiation and contamination with mycoplas...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-02001-x
更新日期:2017-05-15 00:00:00
abstract::In humans, oxytocin (OT) enhances prosocial behaviour. However, it is still unclear how the prosocial effects of OT are modulated by emotional features and/or individuals' characteristics. In a placebo-controlled design, we tested 20 healthy male volunteers to investigate these behavioural and neurophysiological modul...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep00384
更新日期:2012-01-01 00:00:00
abstract::Transplantation of human bone marrow mesenchymal stromal cells (hBM-MSC) promotes functional recovery after stroke in animal models, but the mechanisms underlying these effects remain incompletely understood. We tested the efficacy of Good Manufacturing Practices (GMP) compliant hBM-MSC, injected intravenously 3.5 hou...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-07274-w
更新日期:2017-07-31 00:00:00
abstract::Bacterial blight and bacterial leaf streak are serious, economically damaging, diseases of rice caused by the bacteria Xanthomonas oryzae pv. oryzae and X. oryzae pv. oryzicola. Bacillus amyloliquefaciens FZB42 was shown to possess biocontrol activity against these Xanthomonas strains by producing the antibiotic compo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep12975
更新日期:2015-08-13 00:00:00
abstract::In this work the chalcopyrite CuIn3Se5-xTex (x = 0~0.5) with space group through isoelectronic substitution of Te for Se have been prepared, and the crystal structure dilation has been observed with increasing Te content. This substitution allows the anion position displacement ∆u = 0.25-u to be zero at x ≈ 0.15. Howe...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep40224
更新日期:2017-01-06 00:00:00
abstract::The Y chromosome has high genetic variability with low rates of parallel and back mutations, which make up the most informative haplotyping system. To examine whether Y chromosome haplogroups (Y-hgs) could modify the effects of autosomal variants on non-obstructive azoospermia (NOA), based on our previous genome-wide ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep33363
更新日期:2016-09-15 00:00:00
abstract::Here, we assessed the sulfated derivative of the microbial polysaccharide gellan gum and derivatives of λ and κ-carrageenans for their ability to inhibit Plasmodium falciparum 3D7 and Dd2 growth and invasion of red blood cells in vitro. Growth inhibition was assessed by means of flow cytometry after a 96-h exposure to...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep04723
更新日期:2014-04-17 00:00:00
abstract::Alternations in the p53 regulatory network may render cancer cells resistant to the radiation-induced apoptosis. In this theoretical study we search for the best protocols combining targeted therapy with radiation to treat cancers with wild-type p53, but having downregulated expression of PTEN or overexpression of Wip...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-69380-6
更新日期:2020-07-28 00:00:00
abstract::Key calcifying reef taxa are currently threatened by thermal stress associated with elevated sea surface temperatures (SST) and reduced calcification linked to ocean acidification (OA). Here we undertook an 8 week experimental exposure to near-future climate change conditions and explored the microbiome response of th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep19324
更新日期:2016-01-13 00:00:00
abstract::Although biophysical yield responses to local warming have been studied, we know little about how crop yield growth-a function of climate and technology-responds to global temperature and socioeconomic changes. Here, we present the yield growth of major crops under warming conditions from preindustrial levels as simul...
journal_title:Scientific reports
pub_type: 历史文章,杂志文章
doi:10.1038/s41598-017-08214-4
更新日期:2017-08-10 00:00:00
abstract::Prolonged intake of excessive amounts of ethanol is known to have adverse effects on the central nervous system (CNS). Here we investigated the effects of acute and chronic ethanol exposure and withdrawal from chronic ethanol exposure on glymphatic function, which is a brain-wide metabolite clearance system connected ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-20424-y
更新日期:2018-02-02 00:00:00