Abstract：
:Alzheimer's disease (AD) is characterized by amyloid-β plaques, tau pathology and vascular impairment including pericyte damage. Pericytes are perivascular cells of the blood-brain barrier and can differentiate into different cell types in vitro including microglia. The aim of the present study is to explore if primary mouse brain pericytes isolated and cultured from transgenic AD (APP_SweDI) mice can differentiate into CD11b+ (integrin alpha M) microglia in vitro. We show that primary pericytes (passage 5) isolated from wildtype C57BL6 mice differentiated into CD11b+ microglia (Type B, >90%), when exposed to a differentiation factor mix of FGF-2, cAMP and fibronectin. This differentiation was time-dependent and seen as a large 80 kDa CD11b fragment (days 1-8) and a smaller 50 kDA CD11b fragment (>4 days). These pericytes did not differentiate into neurons, astroglia or oligodendroglia. However, pericytes isolated from transgenic AD mice differentiated into CD11b+ microglia (Type A, <10%) without addition of exogenous differentiation factors, displayed moderate Iba1+ immunostaining and phagocytic activity, but were still positive for PDGFRβ. In conclusion, we show for the first time that primary mouse pericytes from AD mice have the potential to spontanously differentiate in vitro into a CD11b+ microglial-like (Type A) cell type, but we do not provide evidence that these pericytic microglia display a full active microglial cell.
journal_name
Exp Gerontoljournal_title
Experimental gerontologyauthors
Hutter-Schmid B,Humpel Cdoi
10.1016/j.exger.2018.08.003subject
Has Abstractpub_date
2018-10-02 00:00:00pages
30-37eissn
0531-5565issn
1873-6815pii
S0531-5565(18)30435-2journal_volume
112pub_type
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journal_title：Experimental gerontology
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Experimental gerontology
pub_type： 杂志文章,评审
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更新日期：2005-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：2013-04-01 00:00:00
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journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/0531-5565(90)90020-3
更新日期：1990-01-01 00:00:00
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更新日期：2008-10-01 00:00:00
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journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2013.12.006
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pub_type： 杂志文章,评审
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journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2006.07.006
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journal_title：Experimental gerontology
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journal_title：Experimental gerontology
pub_type： 杂志文章,评审
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journal_title：Experimental gerontology
pub_type： 杂志文章
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更新日期：2015-10-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2009-03-01 00:00:00
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journal_title：Experimental gerontology
pub_type： 杂志文章,评审
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journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2003.08.014
更新日期：2004-04-01 00:00:00
