Abstract:
:Baeyer-Villiger monooxygenases (BVMOs) can be used for the biosynthesis of lactones and esters from ketones. However, the BVMO-based biocatalysts are not so stable under process conditions. Thereby, this study focused on enhancing stability of the BVMO-based biocatalysts. The biotransformation of ricinoleic acid into (Z)-11-(heptanoyloxy)undec-9-enoic acid by the recombinant Escherichia coli expressing the BVMO from Pseudomonas putida and an alcohol dehydrogenase from Micrococcus luteus was used as a model system. After thorough investigation of the key factors to influence stability of the BVMO, Cys302 was identified as an engineering target. The substitution of Cys302 to Leu enabled the engineered enzyme (i.e., E6BVMOC302L) to become more stable toward oxidative and thermal stresses. The catalytic activity of E6BVMOC302L-based E. coli biocatalysts was also greater than the E6BVMO-based biocatalysts. Another factor to influence biocatalytic performance of the BVMO-based whole-cell biocatalysts was availability of carbon and energy source during biotransformations. Glucose feeding into the reaction medium led to a marked increase of final product concentrations. Overall, the bioprocess engineering to improve metabolic stability of host cells in addition to the BVMO engineering allowed us to produce (Z)-11-(heptanoyloxy)undec-9-enoic acid to a concentration of 132 mM (41 g/L) from 150 mM ricinoleic acid within 8 h.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Woo JM,Jeon EY,Seo EJ,Seo JH,Lee DY,Yeon YJ,Park JBdoi
10.1038/s41598-018-28575-8subject
Has Abstractpub_date
2018-07-06 00:00:00pages
10280issue
1issn
2045-2322pii
10.1038/s41598-018-28575-8journal_volume
8pub_type
杂志文章abstract::Adipocyte differentiation and function relies on a network of transcription factors, which is disrupted in obesity-associated low grade, chronic inflammation leading to adipose tissue dysfunction. In this context, there is a need for a thorough understanding of the transcriptional regulatory network involved in adipos...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章
doi:10.1038/s41598-017-14570-y
更新日期:2017-10-26 00:00:00
abstract::B cell derived induced pluripotent stem cells (BiPSCs) were recently established from peripheral blood B cells by the simultaneous transfection of Yamanaka factors (Oct3/4, Sox2, Klf4, c-Myc) and C/EBPα using a Sendai virus vector. Here, using a different method, we established BiPSCs with immunoglobulin heavy chain (...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-01627-1
更新日期:2017-05-10 00:00:00
abstract::We investigate whether the collapse of the quantum state of a single photon split between two space-like separated places takes a nonvanishing time. We realize this by using a source of heralded single photons, then splitting the resulting single photon state and letting it propagate over distances much larger than th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-48387-8
更新日期:2019-08-15 00:00:00
abstract::Down syndrome (DS), caused by trisomy of chromosome 21, is one of the most common genetic disorders. Patients with DS display growth retardation and inevitably develop characteristic Alzheimer's disease (AD) neuropathology, including neurofibrillary tangles and neuritic plaques. The expression of amyloid precursor pro...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep22460
更新日期:2016-03-03 00:00:00
abstract::The use of engineered viral strains such as gene therapy vectors and oncolytic viruses (OV) to selectively destroy cancer cells is poised to make a major impact in the clinic and revolutionize cancer therapy. In particular, several studies have shown that OV therapy is safe and well tolerated in humans and can infect ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26786
更新日期:2016-05-26 00:00:00
abstract::Molecular alignment underpins optical, mechanical, and thermal properties of materials, however, its direct measurement from volumes with micrometer dimensions is not accessible, especially, for structurally complex bio-materials. How the molecular alignment is linked to extraordinary properties of silk and its amorph...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-07502-3
更新日期:2017-08-07 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-69584-w
更新日期:2020-07-29 00:00:00
abstract::We show that ATM kinase inhibition using AZ31 prior to 9 or 9.25 Gy total body irradiation (TBI) reduced median time to moribund in mice to 8 days. ATR kinase inhibition using AZD6738 prior to TBI did not reduce median time to moribund. The striking finding associated with ATM inhibition prior to TBI was increased cry...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep41892
更新日期:2017-02-01 00:00:00
abstract::Graphene, a honeycomb lattice of carbon atoms ruled by tight-binding interaction, exhibits extraordinary electronic properties due to the presence of Dirac cones within its band structure. These intriguing singularities have naturally motivated the discovery of their classical analogues. In this work, we present a gen...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-15335-3
更新日期:2017-11-10 00:00:00
abstract::Milk-derived exosomes were identified as a novel mechanism of mother-to-child transmission of regulatory molecules, but their functions in intestinal tissues of neonates are not well-studied. Here, we characterized potential roles of porcine milk-derived exosomes in the intestinal tract. In vitro, treatment with milk-...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep33862
更新日期:2016-09-20 00:00:00
abstract::Giant unilamellar vesicles (GUVs) are increasingly used as a versatile research tool to investigate membrane structure, morphology and phase state. In these studies, GUV preparation is typically enhanced by an externally applied electric field, a process called electroformation. We find that upon osmotic deflation, GU...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-30286-z
更新日期:2018-08-07 00:00:00
abstract::A pancreatic β-cell line MIN6 was previously established in our lab from an insulinoma developed in an IT6 transgenic mouse expressing the SV40 T antigen in β-cells. This cell line has been widely used for in vitro analysis of β-cell function, but tends to lose the mature β-cell features, including glucose-stimulated ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-79992-7
更新日期:2021-01-12 00:00:00
abstract::All-spin logic device (ASLD) has attracted increasing interests as one of the most promising post-CMOS device candidates, thanks to its low power, non-volatility and logic-in-memory structure. Here we investigate the key current-limiting factors and develop a physics-based model of ASLD through nano-magnet switching, ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep14905
更新日期:2015-10-09 00:00:00
abstract::Human pancreatic islet amyloid polypeptide (hIAPP) and beta amyloid (Aβ) can accumulate in Type 2 diabetes (T2D) and Alzheimer's disease (AD) brains and evidence suggests that interaction between the two amyloidogenic proteins can lead to the formation of heterocomplex aggregates. However, the structure and consequenc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-66602-9
更新日期:2020-06-25 00:00:00
abstract::To explore the feasibility of 3D-printed navigation template in proximal femoral varus rotation and shortening osteotomy for older children with developmental dysplasia of the hip (DDH). Between June 2014 and May 2015, navigation templates were designed and used for 12 DDH patients. Surgical information and outcomes w...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep44993
更新日期:2017-03-21 00:00:00
abstract::Upper lip scars are at risk of hypertrophy. Our center therefore uses microporous tape and silicone sheeting for postoperative scar care following cleft lip repair. However, some babies have previously ingested their silicone sheeting, which has the potential for respiratory compromise or gastrointestinal obstruction....
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-25697-x
更新日期:2018-05-09 00:00:00
abstract::In contrast to mammals, adult fish display a remarkable ability to fully regenerate central nervous system (CNS) axons, enabling functional recovery from CNS injury. Both fish and mammals normally undergo a developmental downregulation of axon growth activity as neurons mature. Fish are able to undergo damage-induced ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-50485-6
更新日期:2019-10-02 00:00:00
abstract::C4.4A is a modular glycolipid-anchored Ly6/uPAR/α-neurotoxin multidomain protein that exhibits a prominent membrane-associated expression in stratified squamous epithelia. C4.4A is also expressed in various solid cancer lesions, where high expression levels often are correlated to poor prognosis. Circumstantial eviden...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25833
更新日期:2016-05-12 00:00:00
abstract::Tunneling membrane nanotubes (TnTs) are membrane protrusions connecting nearby or distant cells in vitro and in vivo. Functions of TnTs in cellular processes are various and rely on TnT structure, which also depends on cytoskeletal composition. In the present study, we focused on the organization of microtubules (MTs)...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-35370-y
更新日期:2018-11-20 00:00:00
abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-018-33284-3
更新日期:2018-10-23 00:00:00
abstract::Research on Giardia lamblia has accumulated large information about its molecular cell biology and infection biology. However, giardiasis is still one of the commonest parasitic diarrheal diseases affecting humans. Additionally, an alarming increase in cases refractory to conventional treatment has been reported in lo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-45529-w
更新日期:2019-06-20 00:00:00
abstract::An accurate and efficient solution of the radiative transport equation is proposed for modeling the propagation of photons in the three-dimensional anisotropically scattering half-space medium. The exact refractive index mismatched boundary condition is considered and arbitrary rotationally invariant scattering functi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep02018
更新日期:2013-01-01 00:00:00
abstract::Several studies have shown that the mu opioid receptor (MOR) located in the peripheral nerves can be activated after nerve injury and that it attenuates peripheral nociceptive signals to the spinal dorsal horn. Various cytokines and phosphorylated-p38 (p-p38) activation in the dorsal horn also play an important role i...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25490
更新日期:2016-05-16 00:00:00
abstract::Wide-angle optical systems play a vital role in imaging applications and have been researched for many years. In traditional lenses, attaining a wide field of view (FOV) by using a single optical component is difficult because these lenses have crucial aberrations. In this study, we developed a wide-angle metalens wit...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-72668-2
更新日期:2020-09-24 00:00:00
abstract::The conservation of plant and animal genetic heritage is not a purpose in itself, but it represents the sine qua non condition for practicing a sustainable agriculture and to ensure nutrition and food security on long-term. Our research focused on identifying the areas with the richest genetic diversity of vegetables ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-75413-x
更新日期:2020-10-28 00:00:00
abstract::Antimicrobial resistant infections are a serious public health threat worldwide. Whole genome sequencing approaches to rapidly identify pathogens and predict antibiotic resistance phenotypes are becoming more feasible and may offer a way to reduce clinical test turnaround times compared to conventional culture-based m...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-18972-w
更新日期:2018-01-11 00:00:00
abstract::Low-dimensional behavior of large systems of globally coupled oscillators has been intensively investigated since the introduction of the Ott-Antonsen ansatz. In this report, we generalize the Ott-Antonsen ansatz to second-order Kuramoto models in complex networks. With an additional inertia term, we find a low-dimens...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep04783
更新日期:2014-05-02 00:00:00
abstract::Coriander foliage is a distinctive spice employed on a daily basis in curry and other Indian traditional food preparations mainly for the unique flavour attributes and health benefits. Radical scavenging activity has been demonstrated previously for coriander foliage. However, specific molecules responsible were not i...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-35836-z
更新日期:2018-11-27 00:00:00
abstract::Alzheimer's disease (AD) is a lethal progressive neurological disorder affecting the memory. Recently, US Food and Drug Administration mitigated the standard for drug approval, allowing symptomatic drugs that only improve cognitive deficits to be allowed to accelerate on to clinical trials. Our study focuses on taurin...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep07467
更新日期:2014-12-12 00:00:00
abstract::Alumina toughened zirconia (ATZ) and zirconia toughened alumina (ZTA) are currently the materials of choice to meet the need for tough, strong, and bioinert ceramics for medical devices. However, the mechanical properties of ZrO2/Al2O3 dispersion ceramics could be considerably increased by reducing the corresponding g...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep20589
更新日期:2016-02-05 00:00:00