Association between environmental exposure and CD4+CD25+ regulatory T cells.

Abstract:

BACKGROUND:It is considered that farm areas protect young patients from allergy and asthma due to high exposure to endotoxins. AIM:To compare CD4+/CD25+ T-regulatory cells and forkhead transcription factor Foxp3 expression in asthmatic children allergic to house dust mites (HDM) living in rural and farm areas. MATERIALS AND METHODS:This was a prospective analysis of 35 children living in farm areas (n=19) and rural areas (n=16), aged 8-16, with allergic rhinitis (allergic to dust mites) and newly diagnosed asthma. Surface molecule CD4+CD25+Foxp3+ expression on cultured PBMCs was estimated by flow cytometry using fluorophore-conjugated monoclonal antibodies in each patient. RESULTS:Thirty-five children were included into the analysis: 19 children living in farm areas and 16 in rural areas. Within and between-groups (farm area vs. rural area) differences in CD4+/CD25+ and CD4+/CD25+Foxp3+ cell expression did not reach the level of significance. CONCLUSION:The current analysis showed that CD4+/CD25+ and CD4+/CD25+Foxp3+ cell expression was not associated with place of living in asthmatic children sensitive to HDM.

authors

Fuss P,Bal K,Jerzyńska J,Podlecka D,Stelmach W,Stelmach I

doi

10.1016/j.aller.2018.04.003

subject

Has Abstract

pub_date

2019-01-01 00:00:00

pages

43-46

issue

1

eissn

0301-0546

issn

1578-1267

pii

S0301-0546(18)30073-9

journal_volume

47

pub_type

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