Abstract:
:Endo-1,4-β-mannanase is an enzyme that can catalyze the random hydrolysis of β-1,4-mannosidic linkages in the main chain of mannans, glucomannans and galactomannans and offers many applications in different biotechnology industries. Purification and kinetic properties of the endo-1,4-β-mannanase from recombinant Escherichia coli strain KMAN-3 were examined. Recombinant β-mannanase (KMAN-3) was purified 50.5 fold using Ni-NTA Agarose resin and specific activity of 11900 U/mg protein was obtained. Purified KMAN-3 showed a single band on SDS-PAGE with a molecular weight of 43 kDa. Km and Vmax values of KMAN-3 on ivory nut mannan, locust bean gum, defatted copra meal and konjac glucomannan were 243, 3.83 × 105 37 and 2.13 × 106 mg ml-1 and 2940, 61,100, 3930 and 1.56 × 1010 mg-1, respectively. Carboxymethyl cellulose was not digested by KMAN-3.
journal_name
Protein Expr Purifjournal_title
Protein expression and purificationauthors
Tuntrakool P,Keawsompong Sdoi
10.1016/j.pep.2018.01.009subject
Has Abstractpub_date
2018-06-01 00:00:00pages
23-26eissn
1046-5928issn
1096-0279pii
S1046-5928(17)30685-Xjournal_volume
146pub_type
杂志文章abstract::Human extracellular superoxide dismutase (hEC-SOD) is an enzyme that scavenges reactive oxygen species (ROS). Because of its antioxidant activity, hEC-SOD has been used as a therapeutic protein to treat skin disease and arthritis in mammalian systems. In this study, codon-optimized hEC-SOD was expressed in tobacco (Ni...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2015.11.014
更新日期:2016-03-01 00:00:00
abstract::Streptomyces coelicolor is a soil-dwelling bacterium that undergoes an intricate, saprophytic lifecycle. The bacterium takes up exogenous nucleosides for nucleic acid synthesis or use as carbon and energy sources. However, nucleosides must pass through the membrane with the help of transporters. In the present work, t...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2015.02.004
更新日期:2015-05-01 00:00:00
abstract::In this work we communicate the heterologous expression of a laccase from Coriolopsis gallica in Pichia pastoris. This enzyme has been reported to efficiently degrade a variety of pollutants such as industrial dyes. The expression strategy included using a previously reported modified α-factor preproleader for enhance...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2017.06.001
更新日期:2017-08-01 00:00:00
abstract::A new method for the purification of manganese superoxide dismutase from human liver is described. The procedure involves essentially three steps: DEAE-cellulose, hydroxylapatite, and butyl-Toyopearl chromatographies. The method has several advantages: (i) its simplicity and rapidity (it takes less than 3 days), (ii) ...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/1046-5928(91)90067-s
更新日期:1991-04-01 00:00:00
abstract::Pullulanases are well-known starch-debranching enzymes that are widely used for hydrolysis of a-1,6-glycosidic linkages in starch, pullulan, amylopectin, and other oligosaccharides. Escherichia coli is a popular heterologous expression host for generating target enzymes. However, cells have to be disrupted to obtain t...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2018.09.011
更新日期:2019-03-01 00:00:00
abstract::Aquaporins are integral membrane channel proteins found in all kingdoms of life. The Escherichia coli aquaporin Z (AqpZ) has been shown to solely conduct water at high permeability. Functional AqpZ is generally purified from the membrane fraction. However, the quantity of the purified protein is limited. In this study...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2015.08.014
更新日期:2015-11-01 00:00:00
abstract::Notch signaling plays a key role in cell differentiation and is very well conserved from Drosophila to humans. Ligands of Notch receptors are type I, membrane spanning proteins composed of a large extracellular region and a 100-150 residue cytoplasmic tail. We report here, for the first time, the expression, purificat...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2005.11.027
更新日期:2006-06-01 00:00:00
abstract::The gene for the extremely thermophilic and thermostable 5'-methylthioadenosine phosphorylase from the archaeon Sulfolobus solfataricus was expressed at a high level in Escherichia coli thus providing a basis for detailed structural and functional studies of the enzyme. The recombinant enzyme was purified to homogenei...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1006/prep.1999.1076
更新日期:1999-06-01 00:00:00
abstract::To facilitate studies of multicomponent protein complexes, I have developed an Escherichia coli expression system which coexpresses up to four polypeptides from a single plasmid. The modular nature of the system enables efficient subcloning of a gene into each of the 4 cassettes in the polycistronic expression vector....
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1006/prep.2000.1363
更新日期:2001-02-01 00:00:00
abstract::The hemolytic phospholipase C/sphingomyelinase PlcH from the opportunistic pathogen Pseudomonas aeruginosa represents the founding member of a growing family of virulence factors identified in a wide range of bacterial and fungal pathogens. In P. aeruginosa PlcH is co-expressed with a 17 kDa chaperone (PlcR2) and secr...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2012.11.005
更新日期:2013-07-01 00:00:00
abstract::Leptin is a 16-kDa nonglycosylated hormone that is produced in mature adipocytes and which acts primarily in the hypothalamus to reduce food intake and body weight. While the rat is a representative laboratory animal model in obesity research, so far recombinant rat leptin was not available. In the present study, rat ...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1006/prep.2001.1412
更新日期:2001-06-01 00:00:00
abstract::Envelope glycoprotein E of the dengue virus, which plays a crucial role in its entry into host cells, has an immunogenic domain III (EIII, amino acids 297-394), which is capable of inducing neutralizing antibodies. However, mice immunized with EIII protein without adjuvant elicited low immune responses. To improve low...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2010.07.013
更新日期:2010-12-01 00:00:00
abstract::Thymosin α1-thymopentin (Tα1-TP5) fusion peptide has been proved to be an immune regulator based on its higher immunoregulatory activity than Tα1 and TP5. To obtain Tα1-TP5 more effectively and economically, Tα1-TP5 was genetically fused to a self-cleaving intein-chitin binding domain tag for purification via chitin b...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2012.04.013
更新日期:2012-07-01 00:00:00
abstract::The mRNA encoding the 51-kDa subunit of 6-phosphogluconate dehydrogenase (6PGDH) from sheep liver was reverse-transcribed and amplified. The resulting cDNA was reamplified in N-terminal and C-terminal segments and spliced to generate a full-length clone, and an internal cDNA fragment was also amplified. The full-lengt...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1006/prep.1998.0896
更新日期:1998-07-01 00:00:00
abstract::Recombinant murine MRP14 (mMRP14) was produced in Escherichia coli using the pGEX expression system. The mass of fusion protein, by electrospray ionization-mass spectrometry (ESI/MS), was 39,213 Da which compares well with the theoretical mass (39,210.4 Da). Thrombin digestion of fusion protein was expected at a clone...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1006/prep.1998.1015
更新日期:1999-03-01 00:00:00
abstract::The role of the termini of protein sequences is often perturbed by remnant amino acids after the specific protease cleavage of the affinity tags and/or by the amino acids encoded by the plasmid at/around the restriction enzyme sites used to insert the genes. Here we describe a method for affinity purification of a met...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2019.03.009
更新日期:2019-07-01 00:00:00
abstract::For affinity-chromatography-based purification of proteins that are prone to abnormal termination of translation or that may not be modified at their N-termini, affinity tags are needed which can be fused to the C-terminus. In this publication we describe that maltose binding protein (MBP) fused to the C-terminus of t...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1006/prep.1998.0969
更新日期:1998-12-01 00:00:00
abstract::Phthalate dioxygenase (PDO), a hexamer with one Rieske-type [2Fe-2S] and one Fe (II)-mononuclear center per monomer, and its reductase (PDR), which contains flavin mononucleotide and a plant-type ferredoxin [2Fe-2S] center, are expressed by Burkholderia cepacia at approximately 30mg of crude PDO and approximately 1mg ...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2006.09.004
更新日期:2007-04-01 00:00:00
abstract::A fairly rapid and improved method for producing large amounts of highly pure apoaequorin, the apoprotein of aequorin which emits light on binding Ca2+, is described. The method consists of fusing the gene of the outer membrane protein A (ompA) secretion signal peptide of Escherichia coli to the apoaequorin gene and e...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/1046-5928(91)90060-v
更新日期:1991-04-01 00:00:00
abstract::A recombinant H(C) fragment of botulinum neurotoxin, serotype A (rBoNTA(H(C))), has been successfully expressed in a Mut(+) strain of the methylotrophic yeast Pichia pastoris for use as an antigen in a proposed human vaccine. Fermentation employed glycerol batch, glycerol-fed batch, and methanol-fed batch phases to ac...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1006/prep.2000.1256
更新日期:2000-08-01 00:00:00
abstract::Pen b 26 is one of the allergens produced by Penicillium brevicompactum which is one of the most prevalent in door airborne fungi and a major source of respiratory problems, including asthma. Pen b 26 wa scloned and expressed as an N-terminal as well as a C-terminal His6 tagged fusion protein in Escherichia coli. This...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2009.01.004
更新日期:2009-05-01 00:00:00
abstract::The gene encoding glucose oxidase (GOD) from Aspergillus niger was expressed as a secretory product in the yeast Saccharomyces cerevisiae. Six consecutive histidine residues were fused to the C-terminus of GOD to facilitate purification. The recombinant GOD-His(6) secreted by S. cerevisiae migrated as a broad diffuse ...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/s1046-5928(02)00035-9
更新日期:2002-08-01 00:00:00
abstract::Splicing factor proline- and glutamine-rich (SFPQ) is an RNA-binding protein, playing significant roles in gene regulation and subnuclear body formation. Our recent serendipitous discovery showed that SFPQ binds zinc directly and forms an infinite polymer that is induced by zinc binding to the protein. The zinc-induce...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2020.105626
更新日期:2020-07-01 00:00:00
abstract::The aim of this study was to establish a method of purifying intact complement factor H (CFH) from human plasma. CFH was isolated from human plasma by polyethylene glycol (PEG) precipitation, following three sequential chromatographic columns, which consisted of l-lysine Sepharose column, Resource Q column and Sephacr...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2013.07.014
更新日期:2013-10-01 00:00:00
abstract::The expression of recombinant proteins in bacterial hosts may alter the biophysical properties of the protein of interest as a result of differences in post-translational processing from that observed when produced in the native cell. For example, recombinant human interleukin-1beta (IL-1beta) is produced as three iso...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2005.01.010
更新日期:2005-05-01 00:00:00
abstract::Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), which is capable of selectively inducing apoptosis of cancer cells, is a potential targeted drug for cancer therapy. The TRAIL protein induces apoptosis only in trimeric form. However, the recombinant soluble TRAIL (sTRAIL) trimer has low stability and a...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2015.08.004
更新日期:2015-11-01 00:00:00
abstract::Taxadiene synthase catalyzes the conversion of the universal precursor of diterpenoids, geranylgeranyl diphosphate, to taxadiene, a key intermediate in Taxol (paclitaxel) biosynthesis. The gene encoding taxadiene synthase was cloned recently. Here we report a method for the heterologous overexpression of cDNA encoding...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1006/prep.1998.0870
更新日期:1998-06-01 00:00:00
abstract::A soluble N-terminal domain of the Escherichia coli adenylyl transferase (ATase) is responsible for deadenylylation activity of the intact enzyme. Previous studies of the deadenylylation activity have involved a fragment, AT-N423 (residues 1 to 423), which was extended by 17 amino acids to give AT-N440. This new domai...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2003.11.001
更新日期:2004-03-01 00:00:00
abstract::Ketol-acid reductoisomerase (EC 1.1.1.86) catalyzes the conversion of 2-aceto-2-hydroxyacids to 2-keto-3-hydroxyacids and their subsequent reduction by NADPH to 2,3-dihydroxyacids. The gene encoding the Escherichia coli enzyme was cloned and expressed as a hexahistidine-tagged fusion protein and the recombinant enzyme...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1006/prep.1998.0988
更新日期:1999-02-01 00:00:00
abstract::An expression library was generated from a partial NcoI and HindIII digest of genomic DNA from the thermophilic bacterium, Bacillus stearothermophilus P1. The DNA fragments were cloned into the expression vector pQE-60 and transformed into Escherichia coli M15[EP4]. Sequence analysis of a lipase gene showed an open re...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1006/prep.2001.1456
更新日期:2001-08-01 00:00:00