Expression, purification, crystallization, and preliminary X-ray analysis of the N-terminal domain of Escherichia coli adenylyl transferase.

Abstract:

:A soluble N-terminal domain of the Escherichia coli adenylyl transferase (ATase) is responsible for deadenylylation activity of the intact enzyme. Previous studies of the deadenylylation activity have involved a fragment, AT-N423 (residues 1 to 423), which was extended by 17 amino acids to give AT-N440. This new domain is truncated at the end of a predicted helix and prior to a Q-linker. The domain was found to be very soluble and stable so that it could be purified to homogeneity and crystallized. This construct has deadenylylation activity that is independent of the low nitrogen status indicator PII-UMP. The crystals belong to space group P3(1)21 or its enantiomorph P3(2)21 with a=b=116.6 A and c=67.6 A.

journal_name

Protein Expr Purif

authors

Xu Y,Wen D,Clancy P,Carr PD,Ollis DL,Vasudevan SG

doi

10.1016/j.pep.2003.11.001

keywords:

subject

Has Abstract

pub_date

2004-03-01 00:00:00

pages

142-6

issue

1

eissn

1046-5928

issn

1096-0279

pii

S1046592803003668

journal_volume

34

pub_type

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