Two nucleoside receptors from Streptomyces coelicolor: expression of the genes and characterization of the recombinant proteins.

abstract：:A highly efficient Escherichia coli expression system was established to obtain an appreciable quantity of antihypertensive peptide. The DNA-coding sequence for the Gly-Val-Tyr-Pro-His-Lys peptide was chemically synthesized and linked to form a ten-copy in tandem. It was cloned into the vector pET-15b and expressed in...

journal_title：Protein expression and purification

authors： Wang XL,Ma SN,Yuan YH,Ding Y,Li DS

更新日期：2015-09-01 00:00:00

abstract：:Proteus mirabilis (P. mirabilis) is a zoonotic pathogen that has recently presented a rising infection rate in the poultry industry. To develop an effective vaccine to protect chickens against P. mirabilis infection, OmpA, one of the major outer membrane proteins of P. mirabilis, was expressed in Pichia pastoris. The ...

journal_title：Protein expression and purification

authors： Zhang Y,Yang S,Dai X,Liu L,Jiang X,Shao M,Chi S,Wang C,Yu C,Wei K,Zhu R

更新日期：2015-01-01 00:00:00

abstract：:An Escherichia coli expression system that exploits the bacterial alkaline phosphatase (PhoA) signal sequence to translocate recombinant human epidermal growth factor (hEGF) to the periplasm was used to evaluate how changes in the composition and sequence of amino acids near the PhoA-hEGF junction influence the peripl...

journal_title：Protein expression and purification

authors： Campion SR,Elsasser E,Chung R

更新日期：1997-08-01 00:00:00

abstract：:A strategy for the purification and cleavage of chimeric recombinant proteins based on a genetically engineered metal-binding peptide and a human renin cleavage site is described. Vectors were constructed to direct the synthesis of chimeric human immunodeficiency virus (HIV) reverse transcriptase (RT) or beta-galactos...

journal_title：Protein expression and purification

authors： Evans DB,Tarpley WG,Sharma SK

更新日期：1991-04-01 00:00:00

abstract：:A full-length cDNA sequence of plant type CRY (designated Hae-P-CRY) was cloned from the green alga Haematococcus pluvialis. The cDNA sequence was 3608 base pairs (bp) in length, which contained a 2988-bp open reading frame encoding 995 amino acids with molecular mass of 107.7 kDa and isoelectric point of 6.19. Multip...

journal_title：Protein expression and purification

authors： Hang W,Gujar A,Zhang H,Xu W,Zhao C,Zhu X,Xue J,Zhang C,Ji C,Qin S,Li R,Cui H

更新日期：2020-08-01 00:00:00

abstract：:Hydrophobic charge-induction chromatography (HCIC) using 4-mercaptoethylpyridine (4-MEP) as the ligand is used to purify antibodies. The 4-MEP resin ligand has high affinity for antibodies, which makes it difficult to optimize the elution conditions. Recent studies showed that arginine is effective at eluting and puri...

journal_title：Protein expression and purification

authors： Hirano A,Maruyama T,Shiraki K,Arakawa T,Kameda T

更新日期：2017-01-01 00:00:00

abstract：:Recombinant human interleukin-5 (rhIL-5) was expressed in baculovirus-infected insect cells and purified to homogeneity from the culture medium in a single chromatographic step. Beginning with a cDNA encoding the full-length precursor form of human IL-5, including the authentic secretory leader sequence, recombinant b...

journal_title：Protein expression and purification

authors： Brown PM,Scheid MP,O'Neill GP,Tagari PC,Nicholson DW

更新日期：1995-02-01 00:00:00

abstract：:Cyclohexanone monooxygenase (CMO) is a soluble flavoenzyme originally isolated from Acinetobacter spp. which carries out Baeyer-Villiger reactions with cyclic ketone substrates. In the present study we cloned the Acinetobacter CMO gene and modified it for facile purification from heterologous expression systems by inc...

journal_title：Protein expression and purification

authors： Cheesman MJ,Kneller MB,Kelly EJ,Thompson SJ,Yeung CK,Eaton DL,Rettie AE

更新日期：2001-02-01 00:00:00

abstract：:ABCA4 is a member of the A subfamily of ATP-binding cassette transporters that consists of large integral membrane proteins implicated in inherited human diseases. ABCA4 assists in the clearance of N-retinylidene-phosphatidylethanolamine, a potentially toxic by-product of the visual cycle formed in photoreceptor cells...

journal_title：Protein expression and purification

authors： Tsybovsky Y,Palczewski K

更新日期：2014-05-01 00:00:00

abstract：:Pullulanase is crucial to the specific hydrolysis of branch points in amylopectin and is generally employed as an important enzyme in the starch-processing industry. Recombinant Bacillus subtilis that employs an inducible promoter would be a suitable candidate for pullulanase expression because of its safety and contr...

journal_title：Protein expression and purification

authors： Deng Y,Nie Y,Zhang Y,Wang Y,Xu Y

更新日期：2018-08-01 00:00:00

abstract：:Plants possess very large numbers of biosynthetic cytochrome P450 enzymes. In spite of the importance of these enzymes for the synthesis of bioactive plant secondary metabolites, only two plant P450 structures has been obtained to date. Isoflavone synthase (IFS) is a membrane-associated cytochrome P450 enzyme catalyzi...

journal_title：Protein expression and purification

authors： Chang Z,Wang X,Wei R,Liu Z,Shan H,Fan G,Hu H

更新日期：2010-12-04 00:00:00

abstract：:Arginine has been effectively used in various column chromatographies for improving recovery and resolution, and suppressing aggregation. Here, we have tested the effectiveness of arginine as an eluent in dye-affinity column chromatography using Blue-Sepharose, which binds enzymes requiring adenyl-containing cofactors...

journal_title：Protein expression and purification

authors： Arakawa T,Ejima D,Tsumoto K,Ishibashi M,Tokunaga M

更新日期：2007-04-01 00:00:00

abstract：:The TATA-binding protein (TBP) plays a central role in the assembly of most eukaryotic transcription initiation complexes. We have characterized 3 monoclonal antibodies (mAbs) that react in the far amino-terminal (N-terminal) domain of the human TBP molecule (residues 1-99). One of these mAbs (designated 1TBP22) is a ...

journal_title：Protein expression and purification

authors： Thompson NE,Foley KM,Burgess RR

更新日期：2004-08-01 00:00:00

abstract：:A putative laccase gene (lacG) from Geobacillus sp. JS12 was cloned and expressed as a fusion protein with six histidine residues in Escherichia coli BL21 (DE3) cells, and the protein was primarily found in inclusion bodies. The resulting insoluble proteins were solubilized with 6 M guanidine HCl and refolded using an...

journal_title：Protein expression and purification

authors： Jeon SJ,Park JH

更新日期：2020-09-01 00:00:00

abstract：:Lectins are a class of proteins with specific carbohydrate-binding properties found in a wide variety of plants and animals. Gramineae lectins are presumably defense-related proteins in plants that exert their effect by binding to N-acetylglucosamine. Barley lectin is a vacuolar protein synthesized with an amino-termi...

journal_title：Protein expression and purification

authors： Schroeder MR,Raikhel NV

更新日期：1992-12-01 00:00:00

abstract：:Post-translational modification of proteins is a dynamic way of generating new protein-protein interaction interfaces that are critical for signaling networks in diverse cellular functions. Purified recombinant proteins frequently lack these signature modifications. Using the tumor suppressor p53 as the model protein,...

journal_title：Protein expression and purification

authors： Acharya A,Xu XJ,Husain-Ponnampalam RD,Hoffmann-Benning S,Kuo MH

更新日期：2005-06-01 00:00:00

abstract：:The heat shock protein hsp60 plays a functional role in insulin-dependent diabetes mellitus. The hsp60 epitope p277 (aa 437-aa 460) is effective in vaccinating mice against diabetes. A synthetic peptide gene (p277) that encodes the human hsp60 epitope was cloned to the 3' end of the cellulose-binding domain gene (cbd)...

journal_title：Protein expression and purification

authors： Shpigel E,Elias D,Cohen IR,Shoseyov O

更新日期：1998-11-01 00:00:00

abstract：:A method was developed for the affinity purification of human complement properdin. The preparation is part of an integrated scheme in which over 20 human plasma proteins can be recovered in a highly purified form. The yield of properdin was 5.9 mg from 3 liters of plasma, amounting to a 28% recovery. The crucial step...

journal_title：Protein expression and purification

authors： DiScipio RG

更新日期：1994-04-01 00:00:00

abstract：:The faeB gene encoding the feruloyl esterase B (FAEB) was isolated from Aspergillus niger BRFM131 genomic DNA. The faeB gene, with additional sequence coding for a C-terminal histidine tag, was inserted into an expression vector under the control of the gpd promoter and trpC terminator and expressed in a protease defi...

journal_title：Protein expression and purification

authors： Levasseur A,Benoit I,Asther M,Asther M,Record E

更新日期：2004-09-01 00:00:00

abstract：:Proteins are essential throughout the biological and biomedical sciences and the purification strategies of proteins of interest have advanced over centuries. Elastin-like polypeptides (ELPs) are compound polymers that have recently been highlighted for their sharp and reversible phase transition property when heated ...

journal_title：Protein expression and purification

authors： Fletcher EE,Yan D,Kosiba AA,Zhou Y,Shi H

更新日期：2019-01-01 00:00:00

abstract：:Human granulocyte-macrophage colony-stimulating factor (GM-CSF), a hemopoietic growth factor, was produced and secreted from tobacco cell suspensions. The GM-CSF cDNA was carried by a binary vector under the control of the CaMV 35S promoter and the T7 terminator. In addition, a 5'-nontranslated region from the tobacco...

journal_title：Protein expression and purification

authors： James EA,Wang C,Wang Z,Reeves R,Shin JH,Magnuson NS,Lee JM

更新日期：2000-06-01 00:00:00

abstract：:A purification scheme based on hydrophobic interaction chromatography was developed to separate inactivated foot-and-mouth disease virus (FMDV) from crude supernatant. About 92% recovery and 8.8-fold purification were achieved on Butyl Sepharose 4 FF. Further purification on Superdex 200 resulted in another 29-fold pu...

journal_title：Protein expression and purification

authors： Li H,Yang Y,Zhang Y,Zhang S,Zhao Q,Zhu Y,Zou X,Yu M,Ma G,Su Z

更新日期：2015-09-01 00:00:00

abstract：:The catalase gene of Psychrobacter sp. T-3 was cloned, and the gene product (PktA) was overexpressed in Escherichia coli. The specific activity of the purified PktA was slightly lower than that of the native purified enzyme obtained from Psychrobacter sp. T-3. Spectrophotometric measurements of the purified enzymes su...

journal_title：Protein expression and purification

authors： Kimoto H,Matsuyama H,Yumoto I,Yoshimune K

更新日期：2008-06-01 00:00:00

abstract：:Amidase signature (AS) family amidases are known to exhibit broad substrate specificity. According to the available genome sequence data, a novel AS family amidase, Pl-Ami, was identified and cloned from the genome of Parvibaculum lavamentivorans ZJB14001. The recombinant amidase was overexpressed in Escherichia coli ...

journal_title：Protein expression and purification

authors： Wu ZM,Zheng RC,Zheng YG

更新日期：2017-01-01 00:00:00

abstract：:In order to produce sufficient quantities of fibroblast growth factor-saporin (rFGF-2-SAP) mitotoxin for preclinical evaluation in models of diseases such as cancer and restenosis, we have undertaken the large-scale expression, purification, and characterization of the recombinant molecule. The fusion gene encoding rF...

journal_title：Protein expression and purification

authors： McDonald JR,Ong M,Shen C,Parandoosh Z,Sosnowski B,Bussell S,Houston LL

更新日期：1996-08-01 00:00:00

abstract：:Antifreeze proteins (AFPs) enable organisms to survive under freezing or sub-freezing conditions. AFPs have a great potential in the low temperature storage of cells, tissues, organs, and foods. This process will require a large number of recombinant AFPs. In the present study, the recombinant carrot AFP was highly ex...

journal_title：Protein expression and purification

authors： Zhang DQ,Liu B,Feng DR,He YM,Wang JF

更新日期：2004-06-01 00:00:00

abstract：:Mammalian target of rapamycin (TOR) controls cell growth and metabolism in response to the availability of nutrients, growth factors, and the cellular energy status. Misregulation of TOR can result in a pathogenic increase or decrease in organ size and in cancer. TOR can be inhibited by binding of a complex of rapamyc...

journal_title：Protein expression and purification

authors： Dames SA

更新日期：2008-05-01 00:00:00

abstract：:Plants produce a large number of cellulases that are either secreted or anchored in the plasma membrane where they likely function in various aspects of cellulose synthesis, modification and degradation during plant growth and development. Very few of these enzymes have been characterized in any detail, however. Here ...

journal_title：Protein expression and purification

authors： Tawde MD,Freimuth P

更新日期：2012-10-01 00:00:00

abstract：:Human paraoxonase (hPON3) is a high density lipoprotein-related glycoprotein with multi-enzymatic properties and antioxidant activity which is proposed to participate in the prevention of low density lipoprotein (LDL) oxidation. In this study, hPON3 gene was amplified from Human Fetal Liver Marathon-Ready cDNA and exp...

journal_title：Protein expression and purification

authors： Lu H,Zhu J,Zang Y,Ze Y,Qin J

更新日期：2006-03-01 00:00:00

abstract：:The purification of biologically active human protein synthesis initiation factor 4 alpha, eIF-4 alpha, overexpressed in Escherichia coli, is complicated by its localization in insoluble inclusion bodies, as well as its possession of four cysteines. Two of these cysteines have been reported to be reduced in the native...

journal_title：Protein expression and purification

authors： Stern BD,Wilson M,Jagus R

更新日期：1993-08-01 00:00:00