Abstract:
:The catalase gene of Psychrobacter sp. T-3 was cloned, and the gene product (PktA) was overexpressed in Escherichia coli. The specific activity of the purified PktA was slightly lower than that of the native purified enzyme obtained from Psychrobacter sp. T-3. Spectrophotometric measurements of the purified enzymes suggested that the recombinant PktA contains a mixture of heme b and d, although the native enzyme contains the sole heme b. An addition of the heme precursor 5-aminolevulinic acid (ALA) to the medium increased the heme b content of the recombinant PktA, and the resulting enzyme showed higher specific activity than the native enzyme. This is the first report that shows the heme content of overproduced catalase altered by the host cell growth conditions.
journal_name
Protein Expr Purifjournal_title
Protein expression and purificationauthors
Kimoto H,Matsuyama H,Yumoto I,Yoshimune Kdoi
10.1016/j.pep.2008.03.016subject
Has Abstractpub_date
2008-06-01 00:00:00pages
357-9issue
2eissn
1046-5928issn
1096-0279pii
S1046-5928(08)00092-2journal_volume
59pub_type
杂志文章abstract::NIP1, the product of the avirulence gene AvrRrs1 from Rhynchosporium secalis, a fungal pathogen of barley, is a small secreted cysteine-rich protein. This protein is essential for the specific recognition of the fungus by host plants carrying the complementary resistance gene Rrs1. Different heterologous expression sy...
journal_title:Protein expression and purification
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journal_title:Protein expression and purification
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journal_title:Protein expression and purification
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journal_title:Protein expression and purification
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journal_title:Protein expression and purification
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journal_title:Protein expression and purification
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journal_title:Protein expression and purification
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journal_title:Protein expression and purification
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journal_title:Protein expression and purification
pub_type: 杂志文章,评审
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journal_title:Protein expression and purification
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journal_title:Protein expression and purification
pub_type: 杂志文章
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journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2009.11.003
更新日期:2010-04-01 00:00:00
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journal_title:Protein expression and purification
pub_type: 杂志文章
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journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1006/prep.1994.1026
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journal_title:Protein expression and purification
pub_type: 杂志文章
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journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2006.05.010
更新日期:2006-10-01 00:00:00
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journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2015.12.010
更新日期:2016-04-01 00:00:00
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journal_title:Protein expression and purification
pub_type: 杂志文章
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更新日期:2015-06-01 00:00:00
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journal_title:Protein expression and purification
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journal_title:Protein expression and purification
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journal_title:Protein expression and purification
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journal_title:Protein expression and purification
pub_type: 杂志文章
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journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2006.11.017
更新日期:2007-05-01 00:00:00
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journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1006/prep.1998.0896
更新日期:1998-07-01 00:00:00
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journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2007.10.005
更新日期:2008-02-01 00:00:00
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journal_title:Protein expression and purification
pub_type: 杂志文章
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journal_title:Protein expression and purification
pub_type: 杂志文章
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journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2004.09.021
更新日期:2005-06-01 00:00:00
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journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/1046-5928(90)90041-v
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