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Expression in E. coli and purification of the nucleoside diphosphate kinase b from Leishmania major.

Abstract：

:Leishmaniasis is considered by the World Health Organization to be the second most important disease caused by a protozoan parasite. Biochemical and molecular biology studies can help in the understanding of the biology of the Leishmania parasite. All protozoan parasites, including Leishmania, are unable to synthesize purines de novo, and nucleoside diphosphate kinases (NDK) are involved in the salvage pathway by which free purines are converted to nucleosides and subsequently to nucleotides. In this report, we describe the cloning of NDK coding-sequence from Leishmania major, the expression of the enzyme containing a His(6)-tag in Escherichia coli, and purification of the catalytically active native protein by affinity chromatography using Ni-NTA resin.

journal_name

Protein Expr Purif

journal_title

Protein expression and purification

authors

de Oliveira AH,Ruiz JC,Cruz AK,Greene LJ,Rosa JC,Ward RJ

doi

10.1016/j.pep.2006.05.010

subject

Has Abstract

pub_date

2006-10-01 00:00:00

pages

244-50

issue

2

eissn

1046-5928

issn

1096-0279

pii

S1046-5928(06)00162-8

journal_volume

49

pub_type

杂志文章

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