当前位置： SCI文献检索 > PROTEIN EXPRESSION AND PURIFICATION期刊下所有文献 > Identification of hydroxypyruvate reductase from parsley by peptide sequence comparison after a two-step purification.

Identification of hydroxypyruvate reductase from parsley by peptide sequence comparison after a two-step purification.

Abstract：

:Hydroxypyruvate reductase (HPR), a plant leaf peroxisomal enzyme involved in the glycolate pathway, has been purified in two steps from a crude extract of parsley leaves during the purification of an unrelated ATP-dependent enzyme. HPR, a homogenous side-fraction arising from this purification procedure, was identified after sequencing of three internal peptides which showed near 100% homology with the amino acid sequence deduced from the cDNA encoding the NADH-dependent HPR from cucumber. This is an example of the identification of the activity of an unknown protein through direct sequence work. Some of the parsley HPR physicochemical and kinetic properties are similar to those of the cucumber enzyme.

journal_name

Protein Expr Purif

journal_title

Protein expression and purification

authors

Julliard JH,Breton-Gilet A

doi

10.1006/prep.1996.0666

subject

Has Abstract

pub_date

1997-02-01 00:00:00

pages

10-4

issue

1

eissn

1046-5928

issn

1096-0279

pii

S1046-5928(96)90666-X

journal_volume

9

pub_type

杂志文章

相关文献

PROTEIN EXPRESSION AND PURIFICATION文献大全
  • Heterologous expression, purification, crystallization and preliminary X-ray diffraction analysis of KDO8P synthase from Arabidopsis thaliana.

    abstract：:3-Deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS) [EC 4.1.2.16] is the first and rate-limiting enzyme in the 3-deoxy-d-manno-octulosonate (KDO) biosynthetic pathway. The enzyme is widely expressed in bacteria and plants. Their well conserved protein sequences imply a similar oligomeric arrangement. However, t...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2014.06.010

    authors： Zhang F,Xu Y,Zhang Z

    更新日期：2014-09-01 00:00:00

  • Purification of the medium-chain/long-chain (COT/CPT) carnitine acyltransferase of rat liver microsomes.

    abstract：:A procedure for the purification of the rat liver microsomal carnitine octanoyltransferase (COT) that catalyzes the reversible formation of medium-chain and long-chain acylcarnitines from acyl-coenzyme A is described. The K0.5 for L-carnitine is 0.6 mM and the K0.5 for both decanoyl-CoA and palmitoyl-CoA is 0.6 microM...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/1046-5928(92)90059-6

    authors： Murthy MS,Bieber LL

    更新日期：1992-02-01 00:00:00

  • Production and single-step purification of EGFP and a biotinylated version of the Human Rhinovirus 14 3C protease.

    abstract：:The fluorescent reporter enhanced Green Fluorescent Protein (EGFP) has been used for assaying a wide range of biological activities ranging from gene expression, or localization of target proteins through to intermolecular interactions. However, over-production of this protein in Escherichia coli has resulted in the p...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2011.05.003

    authors： Youell J,Fordham D,Firman K

    更新日期：2011-10-01 00:00:00

  • Isolation, overexpression, and biochemical characterization of the two isoforms of glutamic acid decarboxylase from Escherichia coli.

    abstract：:Escherichia coli glutamic acid decarboxylase is a pyridoxal phosphate-dependent enzyme that catalyzes the alpha-decarboxylation of glutamate to yield 4-amino-butyrate and CO2. The E. coli chromosome contains two genes encoding for this enzyme, gadA and gadB, which map at distinct loci. Their protein products differ in...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1006/prep.1996.0121

    authors： De Biase D,Tramonti A,John RA,Bossa F

    更新日期：1996-12-01 00:00:00

  • Extracellular production of active vibriolysin engineered by random mutagenesis in Escherichia coli.

    abstract：:Vibriolysin, an extracellular protease of Vibrio proteolyticus, is synthesized as a preproenzyme. The N-terminal propeptide functions as an intramolecular chaperone and an inhibitor of the mature enzyme. Extracellular production of recombinant vibriolysin has been achieved in Bacillus subtilis, but not in Escherichia ...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2008.08.001

    authors： Sonoda H,Sugimura A

    更新日期：2008-12-01 00:00:00

  • Affinity purification of native glycodelin from amniotic fluid for biological investigations and development of a glycodelin ELISA for clinical studies.

    abstract：INTRODUCTION:Glycodelin is a glycoprotein with different oligosaccharides that are responsible for its diverse biological functions in contraception and immunosuppression. Therefore, it is necessary to have access to adequate amounts of glycodelin with retained carbohydrate structure for functional studies because the ...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2016.09.020

    authors： Sørensen S,Myrhøj V,Nguyen TH,Aaslo P,Hansen YB

    更新日期：2017-02-01 00:00:00

  • Yeast chromatin reconstitution system using purified yeast core histones and yeast nucleosome assembly protein-1.

    abstract：:Transcription regulation in the cell occurs in the context of chromatin. It follows that a thorough investigation of the mechanism of transcription regulation must take into account the role of chromatin structure. Through classical and molecular genetic experiments in yeast, great strides have been made in understand...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1006/prep.1996.0716

    authors： Pilon J,Terrell A,Laybourn PJ

    更新日期：1997-06-01 00:00:00

  • Purification and characterization of the DNA binding domain of Saccharomyces cerevisiae meiosis-specific transcription factor Ndt80.

    abstract：:Ndt80 is a Saccharomyces cerevisiae meiosis-specific transcription factor responsible for promoting the stage-specific expression of a family of genes referred to as middle sporulation genes. Many members of this gene family are essential for the completion of meiotic chromosome segregation. Thus, Ndt80 is essential f...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2003.08.025

    authors： Sopko R,Stuart DT

    更新日期：2004-01-01 00:00:00

  • A simple and efficient method for generating high-quality recombinant Mical enzyme for in vitro assays.

    abstract：:We have recently identified a new family of multidomain oxidoreductase (redox) enzymes, the MICALs, that directly regulate the actin cytoskeletal elements necessary for the morphology, motility, and trajectory of cells. Our genetic assays reveal that Mical is both necessary and sufficient for actin organization and ce...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2016.05.008

    authors： Wu H,Hung RJ,Terman JR

    更新日期：2016-11-01 00:00:00

  • High-level expression of the Listeria monocytogenes listeriolysin O in Escherichia coli and preliminary characterization of the purified protein.

    abstract：:Listeriolysin O (LLO) is a cholesterol-binding sulfhydryl-activated hemolysin encoded by Listeria monocytogenes hlyA gene. After analyzing the nucleotide coding sequence of this gene from the ATCC 9525 L. monocytogenes strain, we cloned it in a pET vector for expression in Escherichia coli. Thanks to the optimization ...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/s1046-5928(02)00682-4

    authors： Giammarini C,Andreoni F,Amagliani G,Casiere A,Barocci S,Magnani M

    更新日期：2003-03-01 00:00:00

  • The effects of L-arginine on protein stability and DNA binding ability of SaeR, a transcription factor in Staphylococcus aureus.

    abstract：:The SaeRS two-component system in Staphylococcus aureus controls the expression of a series of virulence factors, such as hemolysins, proteases, and coagulase. The response regulator, SaeR, belongs to the OmpR family with an N-terminal regulatory domain and a C-terminal DNA binding domain. To improve the production an...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2020.105765

    authors： Fan R,Shi X,Guo B,Zhao J,Liu J,Quan C,Dong Y,Fan S

    更新日期：2021-01-01 00:00:00

  • Confronting high-throughput protein refolding using high pressure and solution screens.

    abstract：:Over-expression of heterologous proteins in Escherichia coli is commonly hindered by the formation of inclusion bodies. Nevertheless, refolding of proteins in vitro has become an essential requirement in the development of structural genomics (proteomics) and as a means of recovering functional proteins from inclusion...

    journal_title：Protein expression and purification

    pub_type： 杂志文章,评审

    doi：10.1016/j.pep.2007.05.014

    authors： Qoronfleh MW,Hesterberg LK,Seefeldt MB

    更新日期：2007-10-01 00:00:00

  • Expression of bioactive soluble human stem cell factor (SCF) from recombinant Escherichia coli by coproduction of thioredoxin and efficient purification using arginine in affinity chromatography.

    abstract：:Stem cell factor (SCF) known as the c-kit ligand is a two disulfide bridge-containing cytokine in the regulation of the development and function of hematopoietic cell lineages and other cells such as mast cells, germ cells, and melanocytes. The secreted soluble form of SCF exists as noncovalently associated homodimer ...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2014.09.015

    authors： Akuta T,Kikuchi-Ueda T,Imaizumi K,Oshikane H,Nakaki T,Okada Y,Sultana S,Kobayashi K,Kiyokawa N,Ono Y

    更新日期：2015-01-01 00:00:00

  • Coexpression of G-CSF with an unglycosylated G-CSF receptor mutant results in secretion of a stable complex.

    abstract：:Previously, we have shown that the entire extracellular domain of the granulocyte-colony stimulating factor receptor (sG-CSFr) produced in Chinese hamster ovary (CHO) cells forms a stable complex with its ligand G-CSF, at a stoichiometry of 2:2. A truncated receptor molecule consisting of the cytokine receptor homolog...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1006/prep.1998.0942

    authors： Horan TP,Simonet L,Jacobsen R,Mann M,Haniu M,Wen J,Arakawa T,Kuwamoto M,Martin F

    更新日期：1998-10-01 00:00:00

  • High yield expression and purification of isotopically labelled human endothelin-1 for use in NMR studies.

    abstract：:Human endothelin-1 (ET-1) is a potent vasocontractile 21-residue peptide hormone with significant pharmacological importance. An efficient and straightforward expression strategy that enables cost-effective incorporation of stable isotopes is not available thus far. In this report, we describe a cost-effective express...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2006.01.022

    authors： Mac TT,Beyermann M,Pires JR,Schmieder P,Oschkinat H

    更新日期：2006-08-01 00:00:00

  • Soluble expression and purification of a full-length asparaginyl tRNA synthetase from Fasciola gigantica.

    abstract：:We report the molecular cloning, expression, and single-step homogeneous purification of a full-length asparaginyl tRNA synthetase (NRS) from Fasciola gigantica (FgNRS). Fasciola gigantica is a parasitic liver fluke of the class Trematoda. It causes fascioliasis that infects the liver of various mammals, including hum...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2017.10.009

    authors： Vijayakumar R,Tripathi T

    更新日期：2018-03-01 00:00:00

  • Purification of a maize dehydrin.

    abstract：:A maize dehydrin with an apparent molecular weight of 20 kDa was purified from whole kernels of maize inbred line G50. Kernels were ground in a seed mill, stirred overnight in extraction buffer, and centrifuged to extract soluble proteins. The sample was heated to 89 degrees C and centrifuged to remove heat-insoluble ...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1006/prep.1994.1040

    authors： Ceccardi TL,Meyer NC,Close TJ

    更新日期：1994-06-01 00:00:00

  • A screening strategy for heterologous protein expression in Escherichia coli with the highest return of investment.

    abstract：:Heterologous protein expression in Escherichia coli is commonly used to obtain recombinant proteins for a variety of downstream applications. However, many proteins are not, or are only poorly, expressed in soluble form. High level expression often leads to the formation of inclusion bodies and an inactive product tha...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2011.08.030

    authors： Pacheco B,Crombet L,Loppnau P,Cossar D

    更新日期：2012-01-01 00:00:00

  • Cloning, expression and functional characterization of two sesquiterpene synthase genes from moso bamboo (Phyllostachys edulis).

    abstract：:The purpose of this work was to characterize the functions of two sesquiterpene synthase genes from moso bamboo (Phyllostachys edulis). Two novel sesquiterpene synthase genes, belonging to the Tpsa subfamily, were isolated from moso bamboo. MoTPS2 was 1641 bp in length and encoded a protein of 63 kDa, whereas MoTPS6 w...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2015.11.019

    authors： Chen X,Wang Y,Sun J,Wang J,Xun H,Tang F

    更新日期：2016-04-01 00:00:00

  • Toxic misfolding of Arabidopsis cellulases in the secretory pathway of Pichia pastoris.

    abstract：:Plants produce a large number of cellulases that are either secreted or anchored in the plasma membrane where they likely function in various aspects of cellulose synthesis, modification and degradation during plant growth and development. Very few of these enzymes have been characterized in any detail, however. Here ...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2012.08.009

    authors： Tawde MD,Freimuth P

    更新日期：2012-10-01 00:00:00

  • Characterization of detergent purified recombinant rat liver monoamine oxidase B expressed in Pichia pastoris.

    abstract：:The high level expression and purification of rat monoamine oxidase B (rMAOB) in the methylotrophic yeast Pichia pastoris is reported. Nearly 100 mg of purified rMAOB is obtained from 130 g (wet weight) of cells (0.5 L of culture). The MALDI-TOF mass spectrum of the purified protein shows a single species with a molec...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2008.03.002

    authors： Upadhyay AK,Edmondson DE

    更新日期：2008-06-01 00:00:00

  • Intein-mediated expression, purification, and characterization of thymosin α1-thymopentin fusion peptide in Escherichia coli.

    abstract：:Thymosin α1-thymopentin (Tα1-TP5) fusion peptide has been proved to be an immune regulator based on its higher immunoregulatory activity than Tα1 and TP5. To obtain Tα1-TP5 more effectively and economically, Tα1-TP5 was genetically fused to a self-cleaving intein-chitin binding domain tag for purification via chitin b...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2012.04.013

    authors： Li J,Zheng L,Li P,Wang F

    更新日期：2012-07-01 00:00:00

  • CRISPR-Cas9 mediated genetic engineering for the purification of the endogenous integrator complex from mammalian cells.

    abstract：:The Integrator Complex (INT) is a large multi-subunit protein complex, containing at least 14 subunits and a host of associated factors. These protein components have been established through pulldowns of overexpressed epitope tagged subunits or by using antibodies raised against specific subunits. Here, we utilize CR...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2016.08.011

    authors： Baillat D,Russell WK,Wagner EJ

    更新日期：2016-12-01 00:00:00

  • Production of recombinant human lactoferrin in the allantoic fluid of embryonated chicken eggs and its characteristics.

    abstract：:The human iron-binding protein lactoferrin (hLf) has been implicated in a number of important physiological pathways, including those regulating immune function and tumor growth. In an effort to develop an efficient system for production of recombinant hLf (rhLf) that is structurally and functionally equivalent to the...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2009.01.003

    authors： Tutykhina IL,Bezborodova OA,Shmarov MM,Logunov DY,Neugodova GL,Nemtsova ER,Naroditsky BS,Yakubovskaya RI,Gintsburg AL

    更新日期：2009-05-01 00:00:00

  • Rapid generation of stable cell lines expressing corticotropin-releasing hormone receptor for drug discovery.

    abstract：:Human HEK293 cells that stably express the Epstein Barr nuclear antigen 1 (EBNA1) support the episomal replication of plasmids containing the Epstein Barr virus origin of replication (EBV oriP). A 293EBNA (293E) cell line expressing the human corticotropin-releasing hormone receptor subtype I (CRHR1) from an episomal ...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1006/prep.1996.0701

    authors： Horlick RA,Sperle K,Breth LA,Reid CC,Shen ES,Robbins AK,Cooke GM,Largent BL

    更新日期：1997-04-01 00:00:00

  • Cloning, expression, purification, and biochemical characterisation of the FIC motif containing protein of Mycobacterium tuberculosis.

    abstract：:The role of FIC (Filamentation induced by cAMP)(2) domain containing proteins in the regulation of many vital pathways, mostly through the transfer of NMPs from NTPs to specific target proteins (NMPylation), in microorganisms, higher eukaryotes, and plants is emerging. The identity and function of FIC domain containin...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2012.08.020

    authors： Mishra S,Bhagavat R,Chandra N,Vijayarangan N,Rajeswari H,Ajitkumar P

    更新日期：2012-11-01 00:00:00

  • Enhanced soluble expression of recombinant Flavobacterium heparinum heparinase I in Escherichia coli by fusing it with various soluble partners.

    abstract：:Heparinase I (HepA) was originally isolated from Flavobacterium heparinum (F. heparinum) and specifically cleaves heparin/heparan sulfate in a site-dependent manner, showing great promise for producing low molecular weight heparin (LMWH). However, expressing recombinant HepA is extremely difficult in Escherichia coli ...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2012.03.016

    authors： Huang J,Cao L,Guo W,Yuan R,Jia Z,Huang K

    更新日期：2012-06-01 00:00:00

  • Expression in Escherichia coli: purification and characterization of cyclin H, a subunit of the human general transcription/DNA repair factor TFIIH.

    abstract：:The human cyclin H, a protein normally associated with the cyclin-dependent kinase cdk7, was overexpressed in Escherichia coli using a T7 RNA polymerase expression system and further purified to apparent homogeneity. The purified recombinant cyclin H is similar to the endogenous one according to the following criteria...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1006/prep.1996.0693

    authors： Poterszman A,Andersen G,Busso D,Rossignol M,Egly JM,Thierry JC

    更新日期：1997-03-01 00:00:00

  • Enhanced protein expression in the baculovirus/insect cell system using engineered SUMO fusions.

    abstract：:Recombinant protein expression in insect cells varies greatly from protein to protein. A fusion tag that is not only a tool for detection and purification, but also enhances expression and/or solubility would greatly facilitate both structure/function studies and therapeutic protein production. We have shown that fusi...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2008.07.010

    authors： Liu L,Spurrier J,Butt TR,Strickler JE

    更新日期：2008-11-01 00:00:00

  • Increased peptide deformylase activity for N-formylmethionine processing of proteins overexpressed in Escherichia coli: application to homogeneous rubredoxin production.

    abstract：:Deformylation of the initiator N-formylmethionine does not always proceed to completion for proteins overexpressed in Escherichia coli. To overcome this limitation, the def gene encoding the Escherichia coli peptide deformylase was cloned into the plysS plasmid under the tetracycline (Tc) promoter control. The efficie...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2004.03.007

    authors： Tang J,Hernández G,LeMaster DM

    更新日期：2004-07-01 00:00:00