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Cloning, expression and functional characterization of two sesquiterpene synthase genes from moso bamboo (Phyllostachys edulis).

Abstract：

:The purpose of this work was to characterize the functions of two sesquiterpene synthase genes from moso bamboo (Phyllostachys edulis). Two novel sesquiterpene synthase genes, belonging to the Tpsa subfamily, were isolated from moso bamboo. MoTPS2 was 1641 bp in length and encoded a protein of 63 kDa, whereas MoTPS6 was 1626 bp in length, encoded protein 62.4 kDa. Both genes were expressed in Pichia pastoris for heterologous expression, and protein contents reached 0.243 μg μL(-1) for MoTPS2 and 0.088 μg μL(-1) for MoTPS6. The soluble enzymes were catalytically active, and capable of converting farnesyl pyrophosphate to two distinct sesquiterpene compounds. The MoTPS2 gene encoded a farnesol synthase which was responsible for the production of (E, E)-farnesol. MoTPS6 showed nerolidol synthase activity, catalyzing the formation of (E)-nerolidol. Functional characterization of both MoTPSs should prove beneficial for future research into large-scale fermentation of sesquiterpenes.

journal_name

Protein Expr Purif

journal_title

Protein expression and purification

authors

Chen X,Wang Y,Sun J,Wang J,Xun H,Tang F

doi

10.1016/j.pep.2015.11.019

subject

Has Abstract

pub_date

2016-04-01 00:00:00

pages

1-6

eissn

1046-5928

issn

1096-0279

pii

S1046-5928(15)30111-X

journal_volume

120

pub_type

杂志文章

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