当前位置： SCI文献检索 > PROTEIN EXPRESSION AND PURIFICATION期刊下所有文献 > Method for efficient soluble expression and purification of recombinant human interleukin-15.

Method for efficient soluble expression and purification of recombinant human interleukin-15.

Abstract：

:Periplasmic expression of recombinant proteins ensures the production of biologically active proteins in a correctly folded state with several key advantages. This research focused on the in-frame cloning of rhIL-15 in pET-20 (+) vector with pelB-leader sequence to direct the protein to the bacterial periplasm. The target construct periplasmic expression was evaluated in four strains, BL21 (DE3), BL21 (DE3) pLysS, Rosetta 2 (DE3) and Rosetta-gami 2 (DE3). Soluble periplasmic expression of IL-15 was highest in Rosetta-gami 2 (DE3) followed by Rossetta 2 (DE3) whereas negligible expression was observed with rest of two expression host. Best expression clone was selected for purification by dye ligand affinity chromatography. Purified rhIL-15 was characterized by SDS-PAGE, Western blotting and SEC-HPLC. This is the first report of functional recombinant human interleukin-15 being expressed and purified with yield of 120 mg/L in the periplasmic space of E. coli.

journal_name

Protein Expr Purif

journal_title

Protein expression and purification

authors

Ahmed N,Afroze B,Abbas R,Khan MA,Akram M,Tahir S,Bakht S,Munir A,Shahid AA

doi

10.1016/j.pep.2020.105746

subject

Has Abstract

pub_date

2021-01-01 00:00:00

pages

105746

eissn

1046-5928

issn

1096-0279

pii

S1046-5928(20)30337-5

journal_volume

177

pub_type

杂志文章

相关文献

PROTEIN EXPRESSION AND PURIFICATION文献大全
  • Efficient production of recombinant cystatin C using a peptide-tag, 4AaCter, that facilitates formation of insoluble protein inclusion bodies in Escherichia coli.

    abstract：:Cystatin C is a cysteine protease inhibitor produced by a variety of human tissues. The blood concentration of cystatin C depends on the glomerular filtration rate and is an endogenous marker of renal dysfunction. Recombinant cystatin C protein with high immunogenicity is therefore in demand for the diagnostic market....

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2013.01.011

    authors： Hayashi M,Iwamoto S,Sato S,Sudo S,Takagi M,Sakai H,Hayakawa T

    更新日期：2013-04-01 00:00:00

  • Expression and purification of biologically active human granulocyte-macrophage colony stimulating factor (hGM-CSF) using silkworm-baculovirus expression vector system.

    abstract：:Human granulocyte-macrophage colony stimulating factor (hGM-CSF) is a hematopoietic growth factor. It is widely employed as a therapeutic agent targeting neutropenia in cancer patients undergoing chemotherapy and in patients with AIDS or after bone marrow transplantation. In this study, we constructed the recombinant ...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2019.03.010

    authors： Kinoshita Y,Xu J,Masuda A,Minamihata K,Kamiya N,Mon H,Fujita R,Kusakabe T,Lee JM

    更新日期：2019-07-01 00:00:00

  • Expression and purification of milligram levels of inactive G-protein coupled receptors in E. coli.

    abstract：:G-protein coupled receptors (GPCRs) are seven transmembrane helical proteins involved in cell signaling and response. They are targets for many existing therapeutic agents, and numerous drug discovery efforts. Production of large quantities of these receptors for drug screening and structural biology remains challengi...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2006.10.017

    authors： Bane SE,Velasquez JE,Robinson AS

    更新日期：2007-04-01 00:00:00

  • Recovery and characterization of a rotavirus outer capsid protein expressed in a recombinant insect cell system.

    abstract：:Recombinant OSU VP4 protein, and outer capsid antigen of porcine rotavirus, was purified to a high level from the spent broth of baculovirus-infected Spodoptera frugiperda insect cells. Initial clarification of the broth with a 0-60% ammonium sulfate cut retained 93% of the total VP4. Q-sepharose ion exchange chromato...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1006/prep.1993.1030

    authors： Juarbe-Osorio LG,Gorziglia M,Betenbaugh MJ

    更新日期：1993-06-01 00:00:00

  • A mammalian expression vector for expression and purification of secreted proteins for structural studies.

    abstract：:A mammalian expression vector with features optimized for simple expression and purification of secreted proteins has been developed. This vector was constructed to facilitate X-ray crystallographic studies of cysteine-rich glycoproteins that are difficult to express by other means. Proteins expressed with this vector...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1006/prep.2000.1331

    authors： Leahy DJ,Dann CE 3rd,Longo P,Perman B,Ramyar KX

    更新日期：2000-12-01 00:00:00

  • Efficient secretory expression of functional barley limit dextrinase inhibitor by high cell-density fermentation of Pichia pastoris.

    abstract：:The limit dextrinase inhibitor (LDI) from barley seeds acts specifically on limit dextrinase (LD), an endogenous starch debranching enzyme. LDI is a 14 kDa hydrophobic protein containing four disulfide bonds and one unpaired thiol group previously found to be either glutathionylated or cysteinylated. It is a member of...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2011.04.009

    authors： Jensen JM,Vester-Christensen MB,Møller MS,Bønsager BC,Christensen HE,Hachem MA,Svensson B

    更新日期：2011-10-01 00:00:00

  • Heme conjugated magnetic beads to isolate heme-binding proteins from complex mixtures.

    abstract：:The cofactor heme (Fe-protoporphyrin IX) plays many important roles in biology. Identification of novel proteins for the transport, chaperoning and delivery of heme in cells is of widespread interest. Here, we describe the use of heme conjugated magnetic beads for the isolation of heme-binding proteins from complex pr...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2010.10.001

    authors： Jones R,Allen JW

    更新日期：2011-03-01 00:00:00

  • Cloning, expression and functional characterization of two sesquiterpene synthase genes from moso bamboo (Phyllostachys edulis).

    abstract：:The purpose of this work was to characterize the functions of two sesquiterpene synthase genes from moso bamboo (Phyllostachys edulis). Two novel sesquiterpene synthase genes, belonging to the Tpsa subfamily, were isolated from moso bamboo. MoTPS2 was 1641 bp in length and encoded a protein of 63 kDa, whereas MoTPS6 w...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2015.11.019

    authors： Chen X,Wang Y,Sun J,Wang J,Xun H,Tang F

    更新日期：2016-04-01 00:00:00

  • Improving storage stability of recombinant organophosphorus hydrolase.

    abstract：:Organophosphorus hydrolase (OPH) is a ∼38kDa enzyme encoded by opd gene of Flavobacterium sp. The enzyme can hydrolyze and inactivate variety of organophosphate (OP)-compounds, including chemical warfare nerve agents. Thus, OPH is a strong candidate for the development of therapeutic intervention against OP-poisoning ...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2015.01.012

    authors： Satvik Iyengar AR,Tripathy RK,Bajaj P,Pande AH

    更新日期：2015-07-01 00:00:00

  • New high fidelity polymerases from Thermococcus species.

    abstract：:Two DNA polymerase genes have been isolated from Thermococcus strains, Thermococcus zilligii from New Zealand, and the other, Thermococcus 'GT', a fast-growing strain isolated from the Galapagos trench. Both genes were isolated by genomic walking PCR, a technique that does not require expression of the gene product. P...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2006.07.022

    authors： Griffiths K,Nayak S,Park K,Mandelman D,Modrell B,Lee J,Ng B,Gibbs MD,Bergquist PL

    更新日期：2007-03-01 00:00:00

  • Human cell lines: A promising alternative for recombinant FIX production.

    abstract：:Factor IX (FIX) is a vitamin K-dependent protein, and it has become a valuable pharmaceutical in the Hemophilia B treatment. We evaluated the potential of recombinant human FIX (rhFIX) expression in 293T and SK-Hep-1 human cell lines. SK-Hep-1-FIX cells produced higher levels of biologically active protein. The growth...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2015.11.023

    authors： de Sousa Bomfim A,Cristina Corrêa de Freitas M,Picanço-Castro V,de Abreu Soares Neto M,Swiech K,Tadeu Covas D,Maria de Sousa Russo E

    更新日期：2016-05-01 00:00:00

  • Cloning and constitutive expression of His-tagged xylanase GH 11 from Penicillium occitanis Pol6 in Pichia pastoris X33: purification and characterization.

    abstract：:High-level constitutive expression of xylanase GH11 from Penicillium occitanis Pol6 termed PoXyn2 was achieved using the methylotrophic yeast Pichia pastoris. The PoXyn2 cDNA encoding for a mature xylanase of 320 amino acids was subcloned into the pGAPZαA vector, to construct recombinant xylanse with six histidine res...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2012.02.012

    authors： Driss D,Bhiri F,Ghorbel R,Chaabouni SE

    更新日期：2012-05-01 00:00:00

  • Expression and purification of native and functional influenza A virus matrix 2 proton selective ion channel.

    abstract：:Influenza A virus displays one of the highest infection rates of all human viruses and therefore represents a severe human health threat associated with an important economical challenge. Influenza matrix protein 2 (M2) is a membrane protein of the viral envelope that forms a proton selective ion channel. Here we repo...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2016.11.001

    authors： Desuzinges Mandon E,Traversier A,Champagne A,Benier L,Audebert S,Balme S,Dejean E,Rosa Calatrava M,Jawhari A

    更新日期：2017-03-01 00:00:00

  • Expression, purification and in vitro functional reconstitution of the chemokine receptor CCR1.

    abstract：:Chemokine receptors are a specific class of G-protein-coupled receptors (GPCRs) that control cell migration associated with routine immune surveillance, inflammation and development. In addition to their roles in normal physiology, these receptors and their ligands are involved in a large number of inflammatory diseas...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2009.03.001

    authors： Allen SJ,Ribeiro S,Horuk R,Handel TM

    更新日期：2009-07-01 00:00:00

  • Design and purification of active truncated phosphoinositide 3-kinase gamma protein constructs for structural studies.

    abstract：:Phosphoinositide 3-kinase gamma (PI3Kγ) is a lipid kinase that plays a crucial role in cell migration, chemotaxis, oxidative burst and myocardial contractility. It is activated downstream of G protein-coupled receptors (GPCRs) and small GTPases of Ras superfamily. PI3Kγ is a heterodimer composed of a catalytic and a r...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2017.04.011

    authors： Vujičić Žagar A,Scapozza L,Vadas O

    更新日期：2017-07-01 00:00:00

  • Molecular cloning and functional expression of hemolysin from the sea anemone Actineria villosa.

    abstract：:The full-length cDNA that encodes the hemolytic toxin Avt-I, with 226 amino acids, from the venomous sea anemone Actineria villosa has been cloned using the oligo-capping method. The cDNA contains 681bp open reading frame and its predicted amino acid sequences revealed that Avt-I was basic polypeptides without cystein...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2004.12.003

    authors： Uechi G,Toma H,Arakawa T,Sato Y

    更新日期：2005-04-01 00:00:00

  • Purification and characterization of fibrinolytic enzyme from cultured mycelia of Armillaria mellea.

    abstract：:A fibrinolytic enzyme was purified from the cultured mycelia of Armillaria mellea by ion-exchange chromatography followed by gel filtration, and was designated A. mellea metalloprotease (AMMP). The purification protocol resulted in a 627-fold purification of the enzyme, with a final yield of 6.05%. The apparent molecu...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2005.05.004

    authors： Lee SY,Kim JS,Kim JE,Sapkota K,Shen MH,Kim S,Chun HS,Yoo JC,Choi HS,Kim MK,Kim SJ

    更新日期：2005-09-01 00:00:00

  • Protective immunity induced by the vaccination of recombinant Proteus mirabilis OmpA expressed in Pichia pastoris.

    abstract：:Proteus mirabilis (P. mirabilis) is a zoonotic pathogen that has recently presented a rising infection rate in the poultry industry. To develop an effective vaccine to protect chickens against P. mirabilis infection, OmpA, one of the major outer membrane proteins of P. mirabilis, was expressed in Pichia pastoris. The ...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2014.10.001

    authors： Zhang Y,Yang S,Dai X,Liu L,Jiang X,Shao M,Chi S,Wang C,Yu C,Wei K,Zhu R

    更新日期：2015-01-01 00:00:00

  • Affinity purification of native glycodelin from amniotic fluid for biological investigations and development of a glycodelin ELISA for clinical studies.

    abstract：INTRODUCTION:Glycodelin is a glycoprotein with different oligosaccharides that are responsible for its diverse biological functions in contraception and immunosuppression. Therefore, it is necessary to have access to adequate amounts of glycodelin with retained carbohydrate structure for functional studies because the ...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2016.09.020

    authors： Sørensen S,Myrhøj V,Nguyen TH,Aaslo P,Hansen YB

    更新日期：2017-02-01 00:00:00

  • Heterologous expression and purification of a marine alginate lyase in Escherichia coli.

    abstract：:Alginate lyase digestion is an efficient way to degrade alginate into oligosaccharides, which are useful in various areas including chemistry, pharmacy and food industry. Here we determined the sequence of Vibrio sp. QY102 sourced alginate lyase, and set up its heterologous expression in E. coli. We improved its secre...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2018.09.002

    authors： Sun X,Shen W,Gao Y,Cai M,Zhou M,Zhang Y

    更新日期：2019-01-01 00:00:00

  • A combined approach for enhancing the stability of recombinant cis-dihydrodiol naphthalene dehydrogenase from Pseudomonas putida G7 allowed for the structural and kinetic characterization of the enzyme.

    abstract：:The second enzyme of the naphthalene degradation pathway in Pseudomonas putida G7 is NahB, a dehydrogenase that converts cis-1,2-dihydroxy-1,2-dihydronaphthalene to 1,2-dihydroxynaphthalene. We report the cloning, optimization of expression, purification, kinetic studies and preliminary structural characterization of ...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2017.01.005

    authors： Costa DMA,Costa MAF,Guimarães SL,Coitinho JB,Gómez SV,Brandão TADS,Nagem RAP

    更新日期：2017-04-01 00:00:00

  • Expression and purification of functional, recombinant Trypanosoma cruzi complement regulatory protein.

    abstract：:The complement regulatory protein (CRP) of Trypanosoma cruzi is a developmentally regulated glycosylphosphatidylinositol (GPI)-anchored membrane protein that protects the parasite from complement-mediated killing, and is an important virulence determinant of the microorganism. CRP binds human complement components C3b...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/s1046-5928(02)00562-4

    authors： Beucher M,Meira WS,Zegarra V,Galvão LM,Chiari E,Norris KA

    更新日期：2003-01-01 00:00:00

  • Overproduction, purification, and characterization of recombinant bifunctional threonine-sensitive aspartate kinase-homoserine dehydrogenase from Arabidopsis thaliana.

    abstract：:In plant, the first and the third steps of the synthesis of methionine and threonine are catalyzed by a bifunctional enzyme, aspartate kinase-homoserine dehydrogenase (AK-HSDH). In this study, we report the first purification and characterization of a highly active threonine-sensitive AK-HSDH from plants (Arabidopsis ...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1006/prep.2001.1539

    authors： Paris S,Wessel PM,Dumas R

    更新日期：2002-02-01 00:00:00

  • Domain isolation, expression, purification and proteolytic activity of the metalloprotease PrtV from Vibrio cholerae.

    abstract：:The metalloprotease PrtV from Vibrio cholerae serves an important function for the bacteria's ability to invade the mammalian host cell. The protein belongs to the family of M6 proteases, with a characteristic zinc ion in the catalytic active site. PrtV constitutes a 918 amino acids (102 kDa) multidomain pre-pro-prote...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2014.01.012

    authors： Edwin A,Grundström C,Wai SN,Ohman A,Stier G,Sauer-Eriksson AE

    更新日期：2014-04-01 00:00:00

  • Cloning, expression and purification of d-tagatose 3-epimerase gene from Escherichia coli JM109.

    abstract：:An unknown d-tagatose 3-epimerase (DTE) containing a IoIE domain was identified and cloned from Escherichia coli. This gene was subcloned into the prokaryotic expression vector pET-15b, and induced by IPTG in E. coli BL21 expression system. Through His-select gel column purification and fast-protein liquid chromatogra...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2015.06.015

    authors： He X,Zhou X,Yang Z,Xu L,Yu Y,Jia L,Li G

    更新日期：2015-10-01 00:00:00

  • Requirement of continuous transcription for the synthesis of sufficient amounts of protein by a cell-free rapid translation system.

    abstract：:To understand the key processes of cell-free protein synthesis, the synthesis of adipose-type fatty acid binding protein (A-FABP) by a rapid translation system was examined under various conditions. The synthesis of A-FABP was achieved by using an expression vector of A-FABP containing a T7 promoter. However, synthesi...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1006/prep.2001.1570

    authors： Hino M,Shinohara Y,Kajimoto K,Terada H,Baba Y

    更新日期：2002-03-01 00:00:00

  • Extracellular production of active vibriolysin engineered by random mutagenesis in Escherichia coli.

    abstract：:Vibriolysin, an extracellular protease of Vibrio proteolyticus, is synthesized as a preproenzyme. The N-terminal propeptide functions as an intramolecular chaperone and an inhibitor of the mature enzyme. Extracellular production of recombinant vibriolysin has been achieved in Bacillus subtilis, but not in Escherichia ...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2008.08.001

    authors： Sonoda H,Sugimura A

    更新日期：2008-12-01 00:00:00

  • High-level intracellular expression of hydroxynitrile lyase from the tropical rubber tree Hevea brasiliensis in microbial hosts.

    abstract：:(S)-Hydroxynitrile lyase (Hnl) from the tropical rubber tree Hevea brasiliensis catalyzes the formation of (S)-cyanohydrins from hydrocyanic acid and aldehydes or ketones. This enzyme accepts aliphatic, aromatic, and heterocyclic carbonyl compounds as substrates and is therefore considered a potent biocatalyst for the...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1006/prep.1997.0765

    authors： Hasslacher M,Schall M,Hayn M,Bona R,Rumbold K,Lückl J,Griengl H,Kohlwein SD,Schwab H

    更新日期：1997-10-01 00:00:00

  • Insoluble expression of highly soluble halophilic metal binding protein for metal ion biosorption: Application of aggregation-prone peptide from hen egg white lysozyme.

    abstract：:Insoluble expression of intrinsically soluble proteins with native activity is potentially a promising alternative to soluble expression of folded protein or insoluble expression of unfolded protein requiring refolding. Here, we attempted to express highly soluble halophilic His-rich metal binding protein (HP) as inso...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2019.01.001

    authors： Tokunaga M,Arakawa T,Tokunaga Y,Sugimoto Y,Ishibashi M

    更新日期：2019-04-01 00:00:00

  • Recombinant production of a VL single domain antibody in Escherichia coli and analysis of its interaction with peptostreptococcal protein L.

    abstract：:A kappa-light chain from a Fab expression system was truncated by the insertion of a stop codon in the gene sequence to produce a variable light (VL) single domain antibody (dAb). Here, we describe the expression of dAb in the periplasm of Escherichia coli through fermentation in a defined media. Immunoglobulin bindin...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2006.07.013

    authors： Cossins AJ,Harrison S,Popplewell AG,Gore MG

    更新日期：2007-02-01 00:00:00