Abstract:
:Recombinant OSU VP4 protein, and outer capsid antigen of porcine rotavirus, was purified to a high level from the spent broth of baculovirus-infected Spodoptera frugiperda insect cells. Initial clarification of the broth with a 0-60% ammonium sulfate cut retained 93% of the total VP4. Q-sepharose ion exchange chromatography performed at pH 6.5 yielded 67% of the initial amount of VP4 in the pooled fractions, with more than four times the purity of the original sample. Gel filtration chromatography followed ion exchange. VP4 eluted from this column at a volume corresponding to a protein of a molecular weight of approximately 85 kDa, the single chain molecular weight of VP4. This step retained 34% of the initial VP4, with a 28-fold purification. Affinity chromatography, using heparin and glycophorin as ligands, was chosen as a final polishing step. The selective binding of VP4 to the two ligands suggested that VP4 may play a role during in vivo rotaviral infection. These specific interactions based on the biological properties of rotaviruses achieved a VP4 purity level of 85-95%. The overall purification scheme recovered about 1.5 mg per liter of VP4 spent broth from about 50 mg/liter (5% of total protein) present initially in the broth.
journal_name
Protein Expr Purifjournal_title
Protein expression and purificationauthors
Juarbe-Osorio LG,Gorziglia M,Betenbaugh MJdoi
10.1006/prep.1993.1030subject
Has Abstractpub_date
1993-06-01 00:00:00pages
232-9issue
3eissn
1046-5928issn
1096-0279pii
S1046592883710302journal_volume
4pub_type
杂志文章abstract::The hexokinase (ATP;D-hexose 6-phosphotransferase, EC 2.7.1.1) of Schistosoma mansoni has been expressed in Escherichia coli as a fusion protein including an N-terminal polyhistidine tag and enterokinase cleavage site. The enzyme was purified by metal chelate chromatography to > 95% homogeneity, based on analysis by S...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1006/prep.1996.0113
更新日期:1996-11-01 00:00:00
abstract::alpha 1-Acid glycoprotein could be isolated by a one-step extraction method from human sera and plasma. Protein recovered in the water phase after extraction with phenol at 70 degrees C for 20 min was verified as human alpha 1-acid glycoprotein when it was compared with the reference standard human alpha 1-acid glycop...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/1046-5928(91)90006-5
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journal_title:Protein expression and purification
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abstract::Chloroplast transformation systems offer unique advantages in biotechnology, including high level of foreign gene expression, maternal inheritance, and polycistronic expression. We studied chloroplast expression of LTK63 (change Ser-->Lys at position 63 in the A subunit) which is the mutant of Escherichia coli heat-la...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2004.08.002
更新日期:2004-11-01 00:00:00
abstract::A kappa-light chain from a Fab expression system was truncated by the insertion of a stop codon in the gene sequence to produce a variable light (VL) single domain antibody (dAb). Here, we describe the expression of dAb in the periplasm of Escherichia coli through fermentation in a defined media. Immunoglobulin bindin...
journal_title:Protein expression and purification
pub_type: 杂志文章
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abstract::Triacylglycerol hydrolase mobilizes stored triacylglycerol some of which is used for very-low-density lipoprotein assembly in the liver. A full-length cDNA coding for a human triacylglycerol hydrolase (hTGH) was isolated from a human liver cDNA library. The cDNA has an open reading frame of 576 amino acids with a clea...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1006/prep.2001.1553
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abstract::The HIV-1 Gag precursor protein, Pr55(Gag), is a multi-domain polyprotein that drives HIV-1 assembly. The morphological features of HIV-1 suggested Pr55(Gag) assumes a variety of different conformations during virion assembly and maturation, yet structural determination of HIV-1 Pr55(Gag) has not been possible due to ...
journal_title:Protein expression and purification
pub_type: 杂志文章
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abstract::The 42 kDa cleavage product from the carboxyl end of the Plasmodium falciparum merozoite surface protein 1 (MSP1(42)) is an important blood-stage malaria vaccine target. Several recombinant protein expression systems have been used for production of MSP1(42) including yeast (Saccharomyces cerevisiae and Pichia pastori...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2006.06.018
更新日期:2006-11-01 00:00:00
abstract::Protein convertase 1/3 is a serine endoproteinase present in the regulated secretory pathway of endocrine and neuroendocrine cells. It is responsible for the processing of numerous prohormones and proneuropeptides into their biologically active moieties, often following cleavage at pairs of basic residues. The determi...
journal_title:Protein expression and purification
pub_type: 杂志文章
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更新日期:2004-10-01 00:00:00
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journal_title:Protein expression and purification
pub_type: 杂志文章
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journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2004.10.012
更新日期:2005-02-01 00:00:00
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journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2009.06.005
更新日期:2009-11-01 00:00:00
abstract::A crude cell extract from yeast Saccharomyces cerevisae was fractionated by affinity chromatography using the leucine tRNA gene as the recognition site. This approach enables the rapid purification of a protein, which retained its full DNA binding capacity during the enrichment procedure. The active fraction contains ...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/1046-5928(90)90015-q
更新日期:1990-11-01 00:00:00
abstract::Protein asparagine (N)-linked glycosylation is a post-translational modification that occurs in the endoplasmic reticulum; it plays an important role in protein folding, oligomerization, quality control, sorting, and transport. Accordingly, disorders of glycosylation may affect practically every organ system. Dehydrod...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2017.02.001
更新日期:2017-04-01 00:00:00
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journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2015.04.006
更新日期:2015-08-01 00:00:00
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journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2009.03.010
更新日期:2009-08-01 00:00:00
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journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1006/prep.1998.0942
更新日期:1998-10-01 00:00:00
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journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2007.05.016
更新日期:2007-11-01 00:00:00
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journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2018.03.012
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abstract::BTK-2, a 32 residue scorpion toxin initially identified in the venom of red Indian scorpion Mesobuthus tamulus was cloned, overexpressed and purified using Cytochrome b(5) fusion protein system developed in our laboratory. The synthetic gene coding for the peptide was designed taking into account optimal codon usage b...
journal_title:Protein expression and purification
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journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2015.08.029
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abstract::A new procedure for the large-scale purification of the recombinant thermostable chitinase (Chi40) cloned from Streptomyces thermoviolaceus in various expression vectors in Escherichia coli is described. Chi40 was overproduced in the cytosolic and secreted forms. The cytosolic form (Chi40c) was highly overproduced and...
journal_title:Protein expression and purification
pub_type: 杂志文章
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journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2005.09.003
更新日期:2006-03-01 00:00:00
abstract::Lectins are a class of proteins with specific carbohydrate-binding properties found in a wide variety of plants and animals. Gramineae lectins are presumably defense-related proteins in plants that exert their effect by binding to N-acetylglucosamine. Barley lectin is a vacuolar protein synthesized with an amino-termi...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/1046-5928(92)90068-8
更新日期:1992-12-01 00:00:00
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journal_title:Protein expression and purification
pub_type: 杂志文章,评审
doi:10.1016/s1046-5928(02)00641-1
更新日期:2003-03-01 00:00:00
abstract::Human Relaxin 2 is an insulin-related peptide hormone with a mass of 19,084 Da. The mRNA contains a number of arginine codons that are rarely used by Escherichia coli to produce highly expressed proteins. As a result, expressing this recombinant protein in E. coli is problematic. When human Relaxin 2 was expressed in ...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2008.02.016
更新日期:2008-08-01 00:00:00
abstract::Arginase (EC 3.5.3.1; L-arginine amidinohydrolase) is a key enzyme of the urea cycle that catalyses the conversion of arginine to ornithine and urea, which is the final cytosolic reaction of urea formation in the mammalian liver. The recombinant strain of the yeast Saccharomyces cerevisiae that is capable of overprodu...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2011.09.001
更新日期:2012-01-01 00:00:00
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journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1006/prep.2001.1470
更新日期:2001-10-01 00:00:00
abstract::Whey acidic proteins (WAP) belong to a large gene family of antibacterial peptides that perform critical immune system functions. The function of human epididymis protein 4 (HE4), a 124-amino acid long polypeptide that has two whey acidic protein four-disulfide core (WFDC) domains, is not well studied. Here, a fusion ...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2014.08.004
更新日期:2014-10-01 00:00:00
abstract::The numerous proteins occluded within the molluscan shell play a key role in the control of the mineralization process. Although extensively studied, these proteins are still poorly known, mainly because they are difficult to fractionate. In the present paper, we present, for the first time, a simple combined strategy...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1006/prep.2001.1487
更新日期:2001-10-01 00:00:00