Expression and biochemical characterization of recombinant human epididymis protein 4.

abstract：:A method was developed for the affinity purification of human complement properdin. The preparation is part of an integrated scheme in which over 20 human plasma proteins can be recovered in a highly purified form. The yield of properdin was 5.9 mg from 3 liters of plasma, amounting to a 28% recovery. The crucial step...

journal_title：Protein expression and purification

authors： DiScipio RG

更新日期：1994-04-01 00:00:00

abstract：:Staphylococcal enterotoxins (SEs) are powerful superantigens that stimulate non-specific T-cell proliferation produced by Staphylococcus aureus and draw considerable attention as ideal drugs for cancer therapy. The filtrate of S. aureus culture has been used as ampul named Staphylococcal enterotoxin C injection in cli...

journal_title：Protein expression and purification

authors： Pan YQ,Ding D,Li DX,Chen SQ

更新日期：2007-12-01 00:00:00

abstract：:Protein convertase 1/3 is a serine endoproteinase present in the regulated secretory pathway of endocrine and neuroendocrine cells. It is responsible for the processing of numerous prohormones and proneuropeptides into their biologically active moieties, often following cleavage at pairs of basic residues. The determi...

journal_title：Protein expression and purification

authors： Rabah N,Gauthier DJ,Gauthier D,Lazure C

更新日期：2004-10-01 00:00:00

abstract：:The gene coding for phosphoglucomutase (PGM) from Oryctolagus cuniculus (rabbit) has been expressed in Escherichia coli under a T7 expression system with a His-tag. About half of the expressed PGM protein was present in inclusion bodies, but this protein was inactive when solubilized. The protein in the soluble cell f...

journal_title：Protein expression and purification

authors： Chae YK,Markley JL

更新日期：2000-10-01 00:00:00

abstract：:Human HEK293 cells that stably express the Epstein Barr nuclear antigen 1 (EBNA1) support the episomal replication of plasmids containing the Epstein Barr virus origin of replication (EBV oriP). A 293EBNA (293E) cell line expressing the human corticotropin-releasing hormone receptor subtype I (CRHR1) from an episomal ...

journal_title：Protein expression and purification

authors： Horlick RA,Sperle K,Breth LA,Reid CC,Shen ES,Robbins AK,Cooke GM,Largent BL

更新日期：1997-04-01 00:00:00

abstract：:The ubiquitin system represents a selective mechanism for intracellular proteolysis in eukaryotic cells that involves the sequential activity of three enzymes, ubiquitin-activating enzyme (E1), ubiquitin-conjugating enzyme (E2), and ubiquitin-protein ligase (E3). The identification of these proteins and their cellular...

journal_title：Protein expression and purification

authors： Reyes LF,Sommer CA,Beltramini LM,Henrique-Silva F

更新日期：2006-02-01 00:00:00

abstract：:The Pseudomonas sp. have been long recognized for their exogenous lipolytic activities yet the genus still contains a lot of unexplored strains. Due to the versatile metabolic machinery and their potential for adaptation to fluctuating environmental conditions Pseudomonas sp. are of great interest for biotechnological...

journal_title：Protein expression and purification

authors： Priyanka P,Kinsella G,Henehan GT,Ryan BJ

更新日期：2019-01-01 00:00:00

abstract：:The hemolytic phospholipase C/sphingomyelinase PlcH from the opportunistic pathogen Pseudomonas aeruginosa represents the founding member of a growing family of virulence factors identified in a wide range of bacterial and fungal pathogens. In P. aeruginosa PlcH is co-expressed with a 17 kDa chaperone (PlcR2) and secr...

journal_title：Protein expression and purification

authors： Truan D,Vasil A,Stonehouse M,Vasil ML,Pohl E

更新日期：2013-07-01 00:00:00

abstract：:Recombinant protein expression from lac derived promoters by the autoinduction regime is based on diauxic growth of Escherichia coli on glucose and lactose. Glycerol is used as a supporting carbon source during the lactose-induced expression. While this glycerol-based formulation usually provides high cell densities, ...

journal_title：Protein expression and purification

authors： Ukkonen K,Mayer S,Vasala A,Neubauer P

更新日期：2013-10-01 00:00:00

abstract：:The Plasmodium falciparum cysteine protease falcipain-2 is a potential new target for antimalarial chemotherapy. In order to obtain large quantities of active falcipain-2 for biochemical and structural analysis, a systematic assessment of optimal parameters for the expression and refolding of the protease was carried ...

journal_title：Protein expression and purification

authors： Sijwali PS,Brinen LS,Rosenthal PJ

更新日期：2001-06-01 00:00:00

abstract：:We present a straightforward, versatile method for expressing and purifying β-amyloid (Aβ40) and transmembrane peptides derived from β-amyloid precursor protein (Aβ55). In principle, these methods should be applicable to other types of strongly aggregating peptides. We start with a DNA plasmid encoding a HexaHis tag w...

journal_title：Protein expression and purification

authors： Zerweck J,Venkata BS,Pittman JM,Srivastava AK,Moore PC,Sachleben JR,Thinakaran G,Meredith SC

更新日期：2019-10-01 00:00:00

abstract：:The production of a single-chain variable fragment (scFv) antibody against bovine ribonuclease A in the cytoplasm of Escherichia coli trxB/gor double mutant was investigated. Previous reports have shown that the thioredoxin (Trx) protein fusion strategy is useful for the correct folding of scFvs and that the expressio...

journal_title：Protein expression and purification

authors： Sonoda H,Kumada Y,Katsuda T,Yamaji H

更新日期：2010-04-01 00:00:00

abstract：:Lectins are a class of proteins with specific carbohydrate-binding properties found in a wide variety of plants and animals. Gramineae lectins are presumably defense-related proteins in plants that exert their effect by binding to N-acetylglucosamine. Barley lectin is a vacuolar protein synthesized with an amino-termi...

journal_title：Protein expression and purification

authors： Schroeder MR,Raikhel NV

更新日期：1992-12-01 00:00:00

abstract：:Hepatocyte growth factor (HGF) is a multifunctional protein expressed in a variety of cell types and tissues. Here we describe a novel one-step method to separate and identify HGF, based on a unique interaction between HGF and Cu(II). Conditioned medium (CM) from mouse 3T3-L1 adipocytes which contains HGF or purified ...

journal_title：Protein expression and purification

authors： Rahimi N,Etchells S,Elliott B

更新日期：1996-05-01 00:00:00

abstract：:CD23, a 45-kDa type II membrane glycoprotein present on B cells, monocytes, and other human immune cells, is a low-affinity receptor for IgE. The extracellular region of the membrane-bound human CD23 is processed into at least four soluble (s) CD23 forms, with apparent molecular masses of 37, 33, 29, and 25 kDa. High ...

journal_title：Protein expression and purification

authors： Khandekar SS,Mayer RJ,Cusimano DM,Katchur SR,Appelbaum ER

更新日期：2001-07-01 00:00:00

abstract：:Acyl coenzyme A binding protein (ACBP) has been proposed to transport fatty acyl CoAs intracellularly, facilitating their metabolism. In this study, a new mouse recombinant ACBP was produced by insertion of a histidine (his) tag at the C-terminus to allow efficient purification by Ni-affinity chromatography. The his-t...

journal_title：Protein expression and purification

authors： Petrescu AD,Huang H,Hostetler HA,Schroeder F,Kier AB

更新日期：2008-04-01 00:00:00

abstract：:A strategy for the purification and cleavage of chimeric recombinant proteins based on a genetically engineered metal-binding peptide and a human renin cleavage site is described. Vectors were constructed to direct the synthesis of chimeric human immunodeficiency virus (HIV) reverse transcriptase (RT) or beta-galactos...

journal_title：Protein expression and purification

authors： Evans DB,Tarpley WG,Sharma SK

更新日期：1991-04-01 00:00:00

abstract：:We have cloned, from total human liver RNA, the cDNA encoding apolipoprotein E3 (apoE3). Site-directed mutagenesis was used to obtain the cDNA encoding the apoE4 isoform, a major variant of this apolipoprotein in man. These two cDNAs were subcloned into the procaryotic expression vector pAHRS. A polyhistidine tag was ...

journal_title：Protein expression and purification

authors： Pillot T,Barbier A,Visvikis A,Lozac'h K,Rosseneu M,Vandekerckhove J,Siest G

更新日期：1996-06-01 00:00:00

abstract：:High affinity interaction between S-protein and S-peptide fragments of bovine pancreatic RNase A has been recently used for construction of molecular vehicles for targeted drug delivery. The vehicle is assembled as a complex of drug carrier conjugated S-protein with S-peptide-tagged targeting protein. To avoid random ...

journal_title：Protein expression and purification

authors： Backer MV,Gaynutdinov TI,Aloise R,Przekop K,Backer JM

更新日期：2002-12-01 00:00:00

abstract：:Human β-glucuronidase (GUS; EC 3.2.1.31) is a lysosomal enzyme that catalyzes the hydrolysis of β-d-glucuronic acid residues from the non-reducing termini of glycosaminoglycans. Impairment in GUS function leads to the metabolic disorder mucopolysaccharidosis type VII, also known as Sly syndrome. We produced GUS from a...

journal_title：Protein expression and purification

authors： Fratz-Berilla EJ,Ketcham SA,Parhiz H,Ashraf M,Madhavarao CN

更新日期：2017-12-01 00:00:00

abstract：:Antimicrobial peptide LL-37 plays an important role in human body's first line of defense against infection. To better understand the mechanism of action, it is critical to elucidate the three-dimensional structure of LL-37 in complex with bacterial membranes. We present a bacterial expression system that allows the i...

journal_title：Protein expression and purification

authors： Li Y,Li X,Wang G

更新日期：2006-06-01 00:00:00

abstract：:The sequence of the gene from Nocardia sp. NRRL 5646 encoding GTP cyclohydrolase I (GCH), gch, and its adjacent regions was determined. The open reading frame of Nocardia gch contains 684 nucleotides, and the deduced amino acid sequence represents a protein of 227 amino acid residues with a calculated molecular mass o...

journal_title：Protein expression and purification

authors： He A,Simpson DR,Daniels L,Rosazza JP

更新日期：2004-06-01 00:00:00

abstract：:PLD's (Phospholipases D) are ubiquitously expressed proteins involved in many transphosphatidylation reactions. They have a bi-lobed structure composed by two similar domains which at their interface reconstitute the catalytic site through the association of the two conserved HxKx(4)Dx(6)GSxN motifs. PLD1 interacts wi...

journal_title：Protein expression and purification

authors： Viparelli F,Doti N,Sandomenico A,Marasco D,Dathan NA,Miele C,Beguinot F,Monti SM,Ruvo M

更新日期：2008-06-01 00:00:00

abstract：:General procedures for the rapid, large-scale purification of recombinant Lactobacillus casei thymidylate synthase and its mutants have been established. An effective method employs sequential phosphocellulose and hydroxylapatite chromatography. Crude cell extracts are directly applied to phosphocellulose, and the enz...

journal_title：Protein expression and purification

authors： Kealey JT,Santi DV

更新日期：1992-10-01 00:00:00

abstract：:Pen b 26 is one of the allergens produced by Penicillium brevicompactum which is one of the most prevalent in door airborne fungi and a major source of respiratory problems, including asthma. Pen b 26 wa scloned and expressed as an N-terminal as well as a C-terminal His6 tagged fusion protein in Escherichia coli. This...

journal_title：Protein expression and purification

authors： Sevinc MS,Kumar V,Abebe M,Mohottalage S,Kumarathasan P,Vincent R,Vijay HM

更新日期：2009-05-01 00:00:00

abstract：:A highly efficient cell-free translation system has been combined with suppressor tRNA technology to substitute nor-Tyr and 3-fluoro-Tyr in place of Tyr183 at the DNA polymerase active site of p66 of human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT). Supplementing the wild-type HIV-1 p51 RT subunit ...

journal_title：Protein expression and purification

authors： Klarmann GJ,Eisenhauer BM,Zhang Y,Sitaraman K,Chatterjee DK,Hecht SM,Le Grice SF

更新日期：2004-11-01 00:00:00

abstract：:Haloalkane dehalogenase from Xanthobacter autotrophicus was efficiently expressed in Escherichia coli BL21 (DE3) and E. coli JM101. After introduction of restriction sites by PCR the haloalkane dehalogenase gene (dhlA) was translationally fused behind the T7 (phi 10), trc, and tac promoters. This resulted in expressio...

journal_title：Protein expression and purification

authors： Schanstra JP,Rink R,Pries F,Janssen DB

更新日期：1993-10-01 00:00:00

abstract：:l-glutamate oxidase (GLOD), encoded by the gox gene, catalyses the transformation of l-glutamic acid into α-ketoglutaric acid (α-KG). In the present study, Pichia pastoris was used for heterologous production of GLOD following optimization of the gox coding sequence for expression in the yeast host. A series of constr...

journal_title：Protein expression and purification

authors： YaPing W,Ben R,Hong Y,Rui H,Li L,Ping'an L,Lixin M

更新日期：2017-01-01 00:00:00

abstract：:The high specific lysyl endopeptidase (Lys-C; EC 3.4.21.50) is often used for the initial fragmentation of polypeptide chains during protein sequence analysis. However, due to its specificity it could be a useful tool for the production of tailor-made protein hydrolysates with for example bioactive or techno functiona...

journal_title：Protein expression and purification

authors： Stressler T,Eisele T,Meyer S,Wangler J,Hug T,Lutz-Wahl S,Fischer L

更新日期：2016-02-01 00:00:00

abstract：:The recently classified group III trypsins include members like Atlantic cod (Gadus morhua) trypsin Y as well as seven analogues from other cold-adapted fish species. The eight group III trypsins have been characterized from their cDNAs and deduced amino acid sequences but none of the enzymes have been isolated from t...

journal_title：Protein expression and purification

authors： Pálsdóttir HM,Gudmundsdóttir A

更新日期：2007-02-01 00:00:00