Abstract:
:High affinity interaction between S-protein and S-peptide fragments of bovine pancreatic RNase A has been recently used for construction of molecular vehicles for targeted drug delivery. The vehicle is assembled as a complex of drug carrier conjugated S-protein with S-peptide-tagged targeting protein. To avoid random chemical crosslinking of drug carriers to S-protein, we constructed a mutant 16-124aa fragment of RNase A in which 122ala is replaced with a cysteine residue. The mutant and the corresponding wild type fragments expressed in Escherichia coli are refolded into functional conformations only in the presence of S-peptide. After the removal of S-peptide, both fragments retain the ability to bind S-peptide and S-peptide-tagged proteins. The 122cys residue in the mutant fragment is available for site-specific conjugation.
journal_name
Protein Expr Purifjournal_title
Protein expression and purificationauthors
Backer MV,Gaynutdinov TI,Aloise R,Przekop K,Backer JMdoi
10.1016/s1046-5928(02)00546-6keywords:
subject
Has Abstractpub_date
2002-12-01 00:00:00pages
455-61issue
3eissn
1046-5928issn
1096-0279pii
S1046592802005466journal_volume
26pub_type
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