Streamlined procedure for the production of normal and altered versions of recombinant human proinsulin.

abstract：:Human paraoxonase (hPON3) is a high density lipoprotein-related glycoprotein with multi-enzymatic properties and antioxidant activity which is proposed to participate in the prevention of low density lipoprotein (LDL) oxidation. In this study, hPON3 gene was amplified from Human Fetal Liver Marathon-Ready cDNA and exp...

journal_title：Protein expression and purification

authors： Lu H,Zhu J,Zang Y,Ze Y,Qin J

更新日期：2006-03-01 00:00:00

abstract：:Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a promising anticancer agent. The aim of this study is to produce large quantities of highly pure and bioactive recombinant human TRAIL. Here, TRAIL was expressed in soluble form by pH-stat fed-batch cultivation and purified using a rapid and simple tw...

journal_title：Protein expression and purification

authors： Li P,Gu Q,Wu X

更新日期：2016-10-01 00:00:00

abstract：:Plasmids pQE-60 and pQE-30 containing 6 x His-tag sequence were used for expression of fragments of HCV structural and non-structural proteins in Escherichia coli (E. coli). The following fragments were used: core (1-98 aa), NS3 (202-482 aa), and tetramer of hypervariable region 1 (HVR1) of E2 protein. The constructed...

journal_title：Protein expression and purification

authors： Mihailova M,Fiedler M,Boos M,Petrovskis I,Sominskaya I,Roggendorf M,Viazov S,Pumpens P

更新日期：2006-11-01 00:00:00

abstract：:Formation of biominerals often involves specific proteins that modulate the process of matrix assembly, nucleation, and crystal growth. AP7 is an aragonite-associated protein of 7 kDa and is intrinsically disordered. The structural disorder of AP7 makes it very difficult to express in Escherchiacoli. In this work, we ...

journal_title：Protein expression and purification

authors： Huang YC,Chang HH,Mou Y,Chi P,Chan JC,Luo SC

更新日期：2014-08-01 00:00:00

abstract：:The trematode Schistosoma mansoni Sm14 antigen was expressed in the yeast Pichia pastoris and secreted into the culture medium at yields of approximately 250 mg L-1. Sm14 belongs to a family of fatty-acid binding proteins and appears to play an important role in uptake, transport, and compartmentalization of lipids in...

journal_title：Protein expression and purification

authors： Damasceno L,Ritter G,Batt CA

更新日期：2017-06-01 00:00:00

abstract：:Proteins are essential throughout the biological and biomedical sciences and the purification strategies of proteins of interest have advanced over centuries. Elastin-like polypeptides (ELPs) are compound polymers that have recently been highlighted for their sharp and reversible phase transition property when heated ...

journal_title：Protein expression and purification

authors： Fletcher EE,Yan D,Kosiba AA,Zhou Y,Shi H

更新日期：2019-01-01 00:00:00

abstract：:Pullulanase is crucial to the specific hydrolysis of branch points in amylopectin and is generally employed as an important enzyme in the starch-processing industry. Recombinant Bacillus subtilis that employs an inducible promoter would be a suitable candidate for pullulanase expression because of its safety and contr...

journal_title：Protein expression and purification

authors： Deng Y,Nie Y,Zhang Y,Wang Y,Xu Y

更新日期：2018-08-01 00:00:00

abstract：:Human granulocyte-macrophage colony-stimulating factor (GM-CSF), a hemopoietic growth factor, was produced and secreted from tobacco cell suspensions. The GM-CSF cDNA was carried by a binary vector under the control of the CaMV 35S promoter and the T7 terminator. In addition, a 5'-nontranslated region from the tobacco...

journal_title：Protein expression and purification

authors： James EA,Wang C,Wang Z,Reeves R,Shin JH,Magnuson NS,Lee JM

更新日期：2000-06-01 00:00:00

abstract：:The production of a single-chain variable fragment (scFv) antibody against bovine ribonuclease A in the cytoplasm of Escherichia coli trxB/gor double mutant was investigated. Previous reports have shown that the thioredoxin (Trx) protein fusion strategy is useful for the correct folding of scFvs and that the expressio...

journal_title：Protein expression and purification

authors： Sonoda H,Kumada Y,Katsuda T,Yamaji H

更新日期：2010-04-01 00:00:00

abstract：:Procedures are described for the preparation of highly purified thymidylate synthases from Escherichia coli and Bacillus subtilis. The yields in each case are quite high with about 350 mg of pure protein obtained from 1 liter of cells. Basically all that is required to obtain pure enzyme is an induction step from a hi...

journal_title：Protein expression and purification

authors： Changchien LM,Garibian A,Frasca V,Lobo A,Maley GF,Maley F

更新日期：2000-07-01 00:00:00

abstract：:Acetyl-CoA C-acetyltransferase synthase gene (AACT) cDNA, DNA and promoter were cloned from Sanghuangporus baumii. The gene ORF (1260 bp) encoded 419 amino acids. The AACT DNA includes five exons (1-84 bp, 140-513 bp, 570-1027 bp, 1090-1282 bp, 1344-1494 bp) and four introns (85-139 bp, 514-569 bp, 1028-1089 bp, 1283-...

journal_title：Protein expression and purification

authors： Wang X,Wang S,Xu X,Sun J,Ma Y,Liu Z,Sun T,Zou L

更新日期：2020-06-01 00:00:00

abstract：:Ciliary neurotrophic factor (CNTF) is characterized as a neuropoietic cytokine for a broad spectrum of neurons, leading to its evaluation in humans suffering from neurodegenerative diseases. Due to its wide range of biological applications, high yield production of soluble biologically active recombinant human CNTF (r...

journal_title：Protein expression and purification

authors： Wang K,Zhou F,Zhu L,Zhu X,Zhang K,Zhu L

更新日期：2014-04-01 00:00:00

abstract：:Stromal-cell-derived factor-1 (SDF-1alpha) is an 8-kDa chemokine that is constitutively expressed in bone-marrow-derived stromal cells and has been identified as a ligand for the CXCR4 receptor. We produced the chemokine recombinantly as methionine-SDF-1alpha in Escherichia coli without the leader peptide sequence. Th...

journal_title：Protein expression and purification

authors： Holmes WD,Consler TG,Dallas WS,Rocque WJ,Willard DH

更新日期：2001-04-01 00:00:00

abstract：:Human interleukin-7 (IL-7) is a member of the interleukin family. Numerous studies have demonstrated IL-7's effect on B- and T-cell development as well as its potential in various clinical applications. Previously, a study reported that IL-7 could be purified from inclusion bodies using a prokaryotic system, however, ...

journal_title：Protein expression and purification

authors： Luo Y,Kong X,Xu A,Jin S,Wu D

更新日期：2009-01-01 00:00:00

abstract：:A new method for the purification of manganese superoxide dismutase from human liver is described. The procedure involves essentially three steps: DEAE-cellulose, hydroxylapatite, and butyl-Toyopearl chromatographies. The method has several advantages: (i) its simplicity and rapidity (it takes less than 3 days), (ii) ...

journal_title：Protein expression and purification

authors： Matsuda Y,Suzuki K,Ookawara T,Nakata T,Seo HG,Kawata S,Tarui S,Deutsch HF,Taniguchi N

更新日期：1991-04-01 00:00:00

abstract：:L-Threonine dehydrogenase was purified 10,000-fold to a specific activity approximately 300 mumol.min-1.mg-1 protein from porcine liver mitochondria. Purification to apparent homogeneity was achieved by sequential chromatography on DEAE Sepharose FF, Affi-Gel Blue, Sephacryl S-200, Matrex Gel Red A, and Matrex Gel Gre...

journal_title：Protein expression and purification

authors： Kao YC,Davis L

更新日期：1994-10-01 00:00:00

abstract：:We examined the feasibility of high-level production of recombinant human prolactin, a multifunctional protein hormone, in insect cells using a baculovirus expression system. The human prolactin cDNA with and without the secretory signal sequence was cloned into pFastBac1 baculovirus vector under the control of polyhe...

journal_title：Protein expression and purification

authors： Das T,Johns PW,Goffin V,Kelly P,Kelder B,Kopchick J,Buxton K,Mukerji P

更新日期：2000-11-01 00:00:00

abstract：:The protooncogenic serine/threonine protein kinase PKB contains an amino-terminal pleckstrin homology (PH) domain which binds phosphatidylinositides. The PH domain, composed of approximately 100 loosely conserved amino acids, is found in many proteins, including kinases, phospholipases C, GTPases, GTPase-activating pr...

journal_title：Protein expression and purification

authors： Ingley E,Hemmings BA

更新日期：1999-11-01 00:00:00

abstract：:Mannan outer chain N-glycan structures are yeast/fungal-specific typically found on secreted and cell wall glycoproteins. Mannan outer chains consist of an alpha-1,6 polymannose backbone attached to a Man(8-10)(GlcNAc)(2) core. The backbone contains branches of alpha-1,2 mannose residues, terminated with alpha-1,3 man...

journal_title：Protein expression and purification

authors： Rodionov D,Romero PA,Berghuis AM,Herscovics A

更新日期：2009-07-01 00:00:00

abstract：:The availability of recombinant monomeric alkaline phosphatase (AP) is highly desirable in analytical applications involving AP fusion proteins. The cobalt-dependant alkaline phosphatase IV from Bacillus subtilis (BSAP), which was reported to be strongly monomeric, was overexpressed in Escherichia coli using pET autoi...

journal_title：Protein expression and purification

authors： Koksharov M,Lv C,Zhai X,Ugarova N,Huang E

更新日期：2013-08-01 00:00:00

abstract：:Mutant analogues of recombinant human immune interferon (IFN-gamma) with higher stability and biological activity were prepared. Depending on the analogue, protein structure modification might involve introduction of an intramonomer disulfide bond (through replacements of Glu7Cys and Ser69Cys), C-terminal shortening b...

journal_title：Protein expression and purification

authors： Pechenov SE,Tikhonov RV,Shingarova LN,Korobko VG,Yakimov SA,Klyushnichenko VE,Babajantz AA,Beliaev DL,Kuznetzov VP,Shvetz VI,Wulfson AN

更新日期：2002-03-01 00:00:00

abstract：:The P(II) proteins comprise a family of widely distributed signal transduction proteins that integrate the signals of cellular nitrogen, carbon and energy status, and then regulate, by protein-protein interaction, the activity of a variety of target proteins including enzymes, transcriptional regulators and membrane t...

journal_title：Protein expression and purification

authors： Moure VR,Razzera G,Araújo LM,Oliveira MAS,Gerhardt ECM,Müller-Santos M,Almeida F,Pedrosa FO,Valente AP,Souza EM,Huergo LF

更新日期：2012-01-01 00:00:00

abstract：:The numerous proteins occluded within the molluscan shell play a key role in the control of the mineralization process. Although extensively studied, these proteins are still poorly known, mainly because they are difficult to fractionate. In the present paper, we present, for the first time, a simple combined strategy...

journal_title：Protein expression and purification

authors： Marin F,Pereira L,Westbroek P

更新日期：2001-10-01 00:00:00

abstract：:The aim of this study was to establish a method of purifying intact complement factor H (CFH) from human plasma. CFH was isolated from human plasma by polyethylene glycol (PEG) precipitation, following three sequential chromatographic columns, which consisted of l-lysine Sepharose column, Resource Q column and Sephacr...

journal_title：Protein expression and purification

authors： Wang FM,Yu F,Zhao MH

更新日期：2013-10-01 00:00:00

abstract：:Glutathione S-transferase (GST) fusion proteins are widely used in protein production for pure immunogens, protein-protein, and DNA-protein interaction studies. Using basic pGEX vectors, foreign DNA is introduced to the C-terminus of the GST gene and the produced fusion proteins are C-terminally orientated. However, b...

journal_title：Protein expression and purification

authors： Aatsinki JT,Rajaniemi HJ

更新日期：2005-04-01 00:00:00

abstract：:The present report documents an improved enzyme assay method for the mammalian L-alanine:4,5-dioxovalerate transaminase which is of significant utility in work with crude tissue homogenates, cell cultures, or purified enzyme preparations. We also describe a new and rapid purification procedure for this enzyme from rat...

journal_title：Protein expression and purification

authors： Tyagi RK,Datta K

更新日期：1994-12-01 00:00:00

abstract：:Phthalate dioxygenase (PDO), a hexamer with one Rieske-type [2Fe-2S] and one Fe (II)-mononuclear center per monomer, and its reductase (PDR), which contains flavin mononucleotide and a plant-type ferredoxin [2Fe-2S] center, are expressed by Burkholderia cepacia at approximately 30mg of crude PDO and approximately 1mg ...

journal_title：Protein expression and purification

authors： Jaganaman S,Pinto A,Tarasev M,Ballou DP

更新日期：2007-04-01 00:00:00

abstract：:The B-subunits of replicative DNA polymerases belong to the superfamily of calcineurin-like phosphoesterases and are conserved from Archaea to humans. Recently we and others have shown that the B-subunit (DP1) of the archaeal family D DNA polymerase is responsible for proofreading 3'-5' exonuclease activity. The simil...

journal_title：Protein expression and purification

authors： Jokela M,Raki M,Heikkinen K,Sepponen K,Eskelinen A,Syväoja JE

更新日期：2005-09-01 00:00:00

abstract：:A simple and efficient method for hyperexpression in Escherichia coli and purification of capsid protein, p24, of human immunodeficiency virus type 1 (HIV-1) of both B- and C-subtypes is described. DNA-encoding p24 of C-subtype was cloned from C-subtype gag sequence which was obtained by PCR amplification using DNA ex...

journal_title：Protein expression and purification

authors： Gupta S,Arora K,Gupta A,Chaudhary VK

更新日期：2000-08-01 00:00:00

abstract：:Keratinocyte growth factor (KGF) is a member of the fibroblast growth factor family that acts specifically on epithelial cells in a paracrine mode. We employed a mammalian expression system to synthesize recombinant human KGF and isolated two preparations, KGF-a and KGF-b, from medium conditioned by Chinese hamster ov...

journal_title：Protein expression and purification

authors： Hsu YR,Hsu EW,Katta V,Brankow D,Tseng J,Hu S,Morris CF,Kenney WC,Lu HS

更新日期：1998-03-01 00:00:00