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A rapid, high-yield purification of L-alanine:4,5-dioxovalerate transaminase from rat kidney mitochondria using an improved enzyme assay method.

Abstract：

:The present report documents an improved enzyme assay method for the mammalian L-alanine:4,5-dioxovalerate transaminase which is of significant utility in work with crude tissue homogenates, cell cultures, or purified enzyme preparations. We also describe a new and rapid purification procedure for this enzyme from rat kidney mitochondria. The three-step procedure involves the use of digitonin and lubrol for mitochondrial matrix preparation and L-alanine-Sepharose 4B column chromatography followed by gel filtration on Sepharose 6B. By this procedure it is possible to obtain a highly purified enzyme preparation in a relatively short time with a 37.5% yield.

journal_name

Protein Expr Purif

journal_title

Protein expression and purification

authors

Tyagi RK,Datta K

doi

10.1006/prep.1994.1072

subject

Has Abstract

pub_date

1994-12-01 00:00:00

pages

527-33

issue

6

eissn

1046-5928

issn

1096-0279

pii

S1046-5928(84)71072-2

journal_volume

5

pub_type

杂志文章

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