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Efficient expression of SRK intracellular domain by a modeling-based protein engineering.

Abstract：

:S-locus protein kinase (SRK) is a receptor kinase that plays a critical role in self-recognition in the Brassicaceae self-incompatibility (SI) response. SRK is activated by binding of its ligand S-locus protein 11 (SP11) and subsequently induced phosphorylation of the intracellular kinase domain. However, a detailed activation mechanism of SRK is still largely unknown because of the difficulty in stably expressing SRK recombinant proteins. Here, we performed modeling-based protein engineering of the SRK kinase domain for stable expression in Escherichia coli. The engineered SRK intracellular domain was expressed about 54-fold higher production than wild type SRK, without loss of the kinase activity, suggesting it could be useful for further biochemical and structural studies.

journal_name

Protein Expr Purif

journal_title

Protein expression and purification

authors

Murase K,Hirano Y,Takayama S,Hakoshima T

doi

10.1016/j.pep.2015.09.020

subject

Has Abstract

pub_date

2017-03-01 00:00:00

pages

70-75

eissn

1046-5928

issn

1096-0279

pii

S1046-5928(15)30070-X

journal_volume

131

pub_type

杂志文章

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