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Expression in Escherichia coli: purification and characterization of cyclin H, a subunit of the human general transcription/DNA repair factor TFIIH.

Abstract：

:The human cyclin H, a protein normally associated with the cyclin-dependent kinase cdk7, was overexpressed in Escherichia coli using a T7 RNA polymerase expression system and further purified to apparent homogeneity. The purified recombinant cyclin H is similar to the endogenous one according to the following criteria: molecular weight, microsequencing and mass spectra studies, ability to interact with cdk7, and regulatory kinase activity. The scale-up of cyclin H purification is described.

journal_name

Protein Expr Purif

journal_title

Protein expression and purification

authors

Poterszman A,Andersen G,Busso D,Rossignol M,Egly JM,Thierry JC

doi

10.1006/prep.1996.0693

subject

Has Abstract

pub_date

1997-03-01 00:00:00

pages

153-8

issue

2

eissn

1046-5928

issn

1096-0279

pii

S1046-5928(96)90693-2

journal_volume

9

pub_type

杂志文章

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