The fractionation of human plasma proteins. I. Affinity purification of human complement properdin.

abstract：:A kappa-light chain from a Fab expression system was truncated by the insertion of a stop codon in the gene sequence to produce a variable light (VL) single domain antibody (dAb). Here, we describe the expression of dAb in the periplasm of Escherichia coli through fermentation in a defined media. Immunoglobulin bindin...

journal_title：Protein expression and purification

authors： Cossins AJ,Harrison S,Popplewell AG,Gore MG

更新日期：2007-02-01 00:00:00

abstract：:Five variants of human β-defensins (HBDs) were expressed in Escherichia coli using two vector systems (pET28a(+) and pQE30) with inducible expression by IPTG. The last vector has not been previously reported as an expression system for HBDs. The recombinant peptides were different in their lengths and overall charge. ...

journal_title：Protein expression and purification

authors： Corrales-Garcia L,Ortiz E,Castañeda-Delgado J,Rivas-Santiago B,Corzo G

更新日期：2013-05-01 00:00:00

abstract：:The guanine nucleotide exchange factor EF-1beta gene from the thermoacidophilic archaeon Sulfolobus solfataricus (SsEF-1beta) was amplified by PCR and cloned into the pT7-7 expression vector. One of four selected clones harbored the T160C nucleotide substitution leading to the Y54H amino acid change in a hydrophobic r...

journal_title：Protein expression and purification

authors： Ianniciello G,Masullo M,Raimo G,Arcari P,Bocchini V

更新日期：1998-02-01 00:00:00

abstract：:Developmentally regulated G-proteins (DRGs) are a highly conserved family of GTP-binding proteins found in archaea, plants, fungi and animals, indicating important roles in fundamental pathways. Their function is poorly understood, but they have been implicated in cell division, proliferation, and growth, as well as s...

journal_title：Protein expression and purification

authors： O'Connell A,Robin G,Kobe B,Botella JR

更新日期：2009-10-01 00:00:00

abstract：:This article reviews the development of immobilized-metal affinity chromatography (IMAC) and describes its most important applications. We provide an overview on the use of IMAC in protein fractionation and proteomics, in protein immobilization and detection, and on some special applications such as purification of im...

journal_title：Protein expression and purification

authors： Block H,Maertens B,Spriestersbach A,Brinker N,Kubicek J,Fabis R,Labahn J,Schäfer F

更新日期：2011-09-03 00:00:00

abstract：:Serine hydroxymethyltransferase (SHMT) plays a key role in cell physiology as it participates in the different interconversion pathway of folate coenzymes, provides almost exclusively folate one carbon fragments for the biosynthesis of a variety of end products. For the first time, Mycobacterium leprae glyA gene, enco...

journal_title：Protein expression and purification

authors： Sharma S,Bhakuni V

更新日期：2007-09-01 00:00:00

abstract：:Human dermcidin, an anionic antimicrobial peptide expressed in the pons of the brain and the sweat glands, displays antimicrobial activity against pathogenic microorganisms such as Staphylococcus aureus and Candida albicans. Here, we describe the recombinant production of a 48 amino acid dermcidin variant with C-termi...

journal_title：Protein expression and purification

authors： Cipáková I,Gasperík J,Hostinová E

更新日期：2006-02-01 00:00:00

abstract：:Formation of biominerals often involves specific proteins that modulate the process of matrix assembly, nucleation, and crystal growth. AP7 is an aragonite-associated protein of 7 kDa and is intrinsically disordered. The structural disorder of AP7 makes it very difficult to express in Escherchiacoli. In this work, we ...

journal_title：Protein expression and purification

authors： Huang YC,Chang HH,Mou Y,Chi P,Chan JC,Luo SC

更新日期：2014-08-01 00:00:00

abstract：:Escherichia coli tyrosine kinase (Etk) is a membrane bound kinase in gram-negative bacteria that regulates the export of capsular polysaccharides (CPS). The molecular mechanism behind CPS regulation remains unclear, despite access to a crystal structure of the cytoplasmic kinase domain of Etk. In this study, an effici...

journal_title：Protein expression and purification

authors： Chesterman C,Jia Z

更新日期：2016-09-01 00:00:00

abstract：:An Escherichia coli expression system that exploits the bacterial alkaline phosphatase (PhoA) signal sequence to translocate recombinant human epidermal growth factor (hEGF) to the periplasm was used to evaluate how changes in the composition and sequence of amino acids near the PhoA-hEGF junction influence the peripl...

journal_title：Protein expression and purification

authors： Campion SR,Elsasser E,Chung R

更新日期：1997-08-01 00:00:00

abstract：:Targeted anti-tumor toxins consist of a toxic functional moiety that is chemically linked or recombinantly fused to a cell-directing ligand. Ribosome-inactivating proteins (RIPs), especially type I RIPs such as saporin or dianthin, are commonly used as toxin components. Although expression of type I RIP-based fusion p...

journal_title：Protein expression and purification

authors： Gilabert-Oriol R,Thakur M,Weise C,Dernedde J,von Mallinckrodt B,Fuchs H,Weng A

更新日期：2013-09-01 00:00:00

abstract：:L1 is a human cell adhesion glycoprotein involved in the development of the central nervous system that comprises six immunoglobulin-like domains (Ig1-Ig6), five fibronectin-type III (FN1-FN5) domains, a single transmembrane region and a cytoplasmic domain. It contains 20 potential N-glycosylation sites and is heavily...

journal_title：Protein expression and purification

authors： Gouveia RM,Morais VA,Peixoto C,Sousa M,Regalla M,Alves PM,Costa J

更新日期：2007-03-01 00:00:00

abstract：:Stromal-cell-derived factor-1 (SDF-1alpha) is an 8-kDa chemokine that is constitutively expressed in bone-marrow-derived stromal cells and has been identified as a ligand for the CXCR4 receptor. We produced the chemokine recombinantly as methionine-SDF-1alpha in Escherichia coli without the leader peptide sequence. Th...

journal_title：Protein expression and purification

authors： Holmes WD,Consler TG,Dallas WS,Rocque WJ,Willard DH

更新日期：2001-04-01 00:00:00

abstract：:Insulin-like growth factor binding proteins (IGFBPs) display many functions in humans including regulation of the insulin-like growth factor (IGF) signaling pathway. The various roles of human IGFBPs make them attractive protein candidates in drug discovery. Structural and functional knowledge on human proteins with t...

journal_title：Protein expression and purification

authors： Wanscher ASM,Williamson M,Ebersole TW,Streicher W,Wikström M,Cazzamali G

更新日期：2015-04-01 00:00:00

abstract：:Cytosine-specific DNA methyltransferases are important enzymes in most living organisms. In prokaryotes, most DNA methyltransferases are members of the type II restriction-modification system where they methylate host DNA, thereby protecting it from digestion by the accompanying restriction endonucleases. DNA methyltr...

journal_title：Protein expression and purification

authors： Grgic M,Williamson A,Kjæreng Bjerga GE,Altermark B,Leiros I

更新日期：2018-10-01 00:00:00

abstract：:We have developed a mammalian expression system suitable for the production of enzymatically biotinylated integral membrane proteins. The key feature of this system is the doxycycline (dox)-regulated co-expression of a secreted variant of Escherichia coli biotin ligase (BirA) and a target protein with a 13-residue bio...

journal_title：Protein expression and purification

authors： Mize GJ,Harris JE,Takayama TK,Kulman JD

更新日期：2008-02-01 00:00:00

abstract：:Disulfide bonds are normally formed after a polypeptide has been exported from the reducing environment of the cytoplasm into a more oxidizing compartment, such as the bacterial periplasm. Recently, we showed that in Escherichia coli trxB gor mutants, in which the reduction of thioredoxin and glutathione is impaired, ...

journal_title：Protein expression and purification

authors： Levy R,Weiss R,Chen G,Iverson BL,Georgiou G

更新日期：2001-11-01 00:00:00

abstract：:The sequence of the gene from Nocardia sp. NRRL 5646 encoding GTP cyclohydrolase I (GCH), gch, and its adjacent regions was determined. The open reading frame of Nocardia gch contains 684 nucleotides, and the deduced amino acid sequence represents a protein of 227 amino acid residues with a calculated molecular mass o...

journal_title：Protein expression and purification

authors： He A,Simpson DR,Daniels L,Rosazza JP

更新日期：2004-06-01 00:00:00

abstract：:The preparation of a pure and homogeneous protein sample at proper concentration is a prerequisite for success when attempting their crystallization for structural determination. The detergents suitable for solubilization particularly of membrane proteins are not always the best for crystallization. Myelin of the peri...

journal_title：Protein expression and purification

authors： Sedzik J,Uyemura K,Tsukihara T

更新日期：2002-12-01 00:00:00

abstract：:Splicing factor proline- and glutamine-rich (SFPQ) is an RNA-binding protein, playing significant roles in gene regulation and subnuclear body formation. Our recent serendipitous discovery showed that SFPQ binds zinc directly and forms an infinite polymer that is induced by zinc binding to the protein. The zinc-induce...

journal_title：Protein expression and purification

authors： Lim YW,Lee M

更新日期：2020-07-01 00:00:00

abstract：:Human pancreatic ribonuclease (HP-RNase) has considerable promise as a therapeutic agent. Structure-function analyses of HP-RNase have been impeded by the difficulty of obtaining the enzyme from its host. Here, a gene encoding HP-RNase was designed, synthesized, and inserted into two expression vectors that then direc...

journal_title：Protein expression and purification

authors： Ribó M,delCardayré SB,Raines RT,de Llorens R,Cuchillo CM

更新日期：1996-05-01 00:00:00

abstract：:Mammalian target of rapamycin (TOR) controls cell growth and metabolism in response to the availability of nutrients, growth factors, and the cellular energy status. Misregulation of TOR can result in a pathogenic increase or decrease in organ size and in cancer. TOR can be inhibited by binding of a complex of rapamyc...

journal_title：Protein expression and purification

authors： Dames SA

更新日期：2008-05-01 00:00:00

abstract：:Ndt80 is a Saccharomyces cerevisiae meiosis-specific transcription factor responsible for promoting the stage-specific expression of a family of genes referred to as middle sporulation genes. Many members of this gene family are essential for the completion of meiotic chromosome segregation. Thus, Ndt80 is essential f...

journal_title：Protein expression and purification

authors： Sopko R,Stuart DT

更新日期：2004-01-01 00:00:00

abstract：:Human HEK293 cells that stably express the Epstein Barr nuclear antigen 1 (EBNA1) support the episomal replication of plasmids containing the Epstein Barr virus origin of replication (EBV oriP). A 293EBNA (293E) cell line expressing the human corticotropin-releasing hormone receptor subtype I (CRHR1) from an episomal ...

journal_title：Protein expression and purification

authors： Horlick RA,Sperle K,Breth LA,Reid CC,Shen ES,Robbins AK,Cooke GM,Largent BL

更新日期：1997-04-01 00:00:00

abstract：:This study describes comparison between IPTG and lactose induction on expression of caprine growth hormone (cGH), enhancing cell densities of Escherichia coli cultures and refolding the recombinant cGH, produced as inclusion bodies, to biologically active state. 2-3 times higher cell densities were obtained in shake f...

journal_title：Protein expression and purification

authors： Khan MA,Sadaf S,Sajjad M,Waheed Akhtar M

更新日期：2009-11-01 00:00:00

abstract：:Na+, K+-ATPase beta2 subunit (NKA1b2) is not only a regulator of Na+, K+-ATPase, but also functions in the interaction between neuron and glia cells as a Ca2+-dependent adhesion molecule. To further study the function of NKA1b2, the anti-NKA1b2 polyclonal antibody was prepared to recognize the outer-membrane carboxyl ...

journal_title：Protein expression and purification

authors： Chen TF,Zhang YL,Xu WL,Li ZQ,Hou B,Wang CL,Fan M,Qian LJ,Zhou RP,Zhang CG

更新日期：2004-09-01 00:00:00

abstract：:The purification of biologically active human protein synthesis initiation factor 4 alpha, eIF-4 alpha, overexpressed in Escherichia coli, is complicated by its localization in insoluble inclusion bodies, as well as its possession of four cysteines. Two of these cysteines have been reported to be reduced in the native...

journal_title：Protein expression and purification

authors： Stern BD,Wilson M,Jagus R

更新日期：1993-08-01 00:00:00

abstract：:The main principles of higher-order protein oligomerization are elucidated by many structural and biophysical studies. An astonishing number of proteins self-associate to form dimers or higher-order quaternary structures which further interact with other biomolecules to elicit complex cellular responses. In this study...

journal_title：Protein expression and purification

authors： Pandhare A,Stuebler AG,Pirayesh E,Jansen M

更新日期：2019-01-01 00:00:00

abstract：:Streptococcal protein G has been used extensively for the purification of antibodies using the interaction of the Fc region with protein G. Many antibodies also interact with protein G through a low-affinity binding site for the Fab region. The exploitation of this low-affinity interaction for the purification of Fab'...

journal_title：Protein expression and purification

authors： Proudfoot KA,Torrance C,Lawson AD,King DJ

更新日期：1992-10-01 00:00:00

abstract：:A crude cell extract from yeast Saccharomyces cerevisae was fractionated by affinity chromatography using the leucine tRNA gene as the recognition site. This approach enables the rapid purification of a protein, which retained its full DNA binding capacity during the enrichment procedure. The active fraction contains ...

journal_title：Protein expression and purification

authors： Kaufmann E

更新日期：1990-11-01 00:00:00