Abstract：
:Developmentally regulated G-proteins (DRGs) are a highly conserved family of GTP-binding proteins found in archaea, plants, fungi and animals, indicating important roles in fundamental pathways. Their function is poorly understood, but they have been implicated in cell division, proliferation, and growth, as well as several medical conditions. Individual subfamilies within the G-protein superfamily possess unique nucleotide binding and hydrolysis rates that are intrinsic to their cellular function, and so characterization of these rates for a particular G-protein may provide insight into its cellular activity. We have produced recombinant active DRG protein using a bacterial expression system and refolding, and performed biochemical characterization of their GTP binding and hydrolysis. We show that recombinant Arabidopsis thaliana atDRG1 and atDRG2a are able to bind GDP and GTP. We also show that DRGs can hydrolyze GTP in vitro without the assistance of GTPase-activating proteins and guanine exchange factors. The atDRG proteins hydrolyze GTP at a relatively slow rate (0.94x10(-3)min(-1) for DRG1 and 1.36x10(-3)min(-1) for DRG2) that is consistent with their nearest characterized relatives, the Obg subfamily. The ability of DRGs to bind nucleotide substrates without assistance, their slow rate of GTP hydrolysis, heat stress activation and domain conservation suggest a possible role as a chaperone in ribosome assembly in response to stress as it has been suggested for the Obg proteins, a different but related G-protein subfamily.
journal_name
Protein Expr Purifjournal_title
Protein expression and purificationauthors
O'Connell A,Robin G,Kobe B,Botella JRdoi
10.1016/j.pep.2009.05.009subject
Has Abstractpub_date
2009-10-01 00:00:00pages
88-95issue
2eissn
1046-5928issn
1096-0279pii
S1046-5928(09)00132-6journal_volume
67pub_type
杂志文章abstract：:Envelope glycoprotein E of the dengue virus, which plays a crucial role in its entry into host cells, has an immunogenic domain III (EIII, amino acids 297-394), which is capable of inducing neutralizing antibodies. However, mice immunized with EIII protein without adjuvant elicited low immune responses. To improve low...
journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1016/j.pep.2010.07.013
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journal_title：Protein expression and purification
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abstract：:Hendra virus (family Paramyxoviridae) is a negative sense single-stranded RNA virus (NSRV) which has been found to cause disease in humans, horses, and experimentally in other animals, e.g. pigs and cats. Pteropid bats commonly known as flying foxes have been identified as the natural host reservoir. The Hendra virus ...
journal_title：Protein expression and purification
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doi：10.1016/j.pep.2015.07.008
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journal_title：Protein expression and purification
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journal_title：Protein expression and purification
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journal_title：Protein expression and purification
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journal_title：Protein expression and purification
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journal_title：Protein expression and purification
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journal_title：Protein expression and purification
pub_type： 杂志文章
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journal_title：Protein expression and purification
pub_type： 杂志文章
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journal_title：Protein expression and purification
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journal_title：Protein expression and purification
pub_type： 杂志文章
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更新日期：2004-08-01 00:00:00
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journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1016/j.pep.2017.04.002
更新日期：2017-06-01 00:00:00
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journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1016/j.pep.2013.07.016
更新日期：2013-10-01 00:00:00
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journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1016/j.pep.2007.04.017
更新日期：2007-09-01 00:00:00
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journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1016/s1046-5928(02)00564-8
更新日期：2002-12-01 00:00:00
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journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1016/j.pep.2007.10.021
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journal_title：Protein expression and purification
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journal_title：Protein expression and purification
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journal_title：Protein expression and purification
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journal_title：Protein expression and purification
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更新日期：2010-08-01 00:00:00
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journal_title：Protein expression and purification
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journal_title：Protein expression and purification
pub_type： 杂志文章
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更新日期：2011-09-03 00:00:00
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journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1006/prep.1997.0827
更新日期：1998-03-01 00:00:00
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journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1016/j.pep.2006.06.018
更新日期：2006-11-01 00:00:00
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journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1006/prep.1997.0741
更新日期：1997-08-01 00:00:00
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journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1016/j.pep.2009.06.005
更新日期：2009-11-01 00:00:00
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journal_title：Protein expression and purification
pub_type： 杂志文章
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更新日期：2003-10-01 00:00:00
abstract：:Strategies for the expression of precursors of eukaryotic secreted proteins as part of fused proteins in Escherichia coli have been explored. A fusion protein with beta-galactosidase at the N-terminal end and honeybee prepromelittin at the C-terminal end (beta-gal-pM) was expressed in low amounts as a cleaved polypept...
journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1016/1046-5928(91)90095-z
更新日期：1991-10-01 00:00:00
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journal_title：Protein expression and purification
pub_type： 杂志文章
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更新日期：2007-12-01 00:00:00
