Heterologous expression and in vitro assembly of the transmembrane cytochrome b6.

abstract：:To investigate the expression and purification of an unstable heterologous protein in Pichia pastoris, the cDNA of H5-lysozyme, a hen egg lysozyme mutant with a hydrophobic pentapeptide (Phe-Phe-Val-Ala-Pro) fused to the carboxyl terminus, was integrated into the genome of P. pastoris. It was found that medium composi...

journal_title：Protein expression and purification

authors： Liu ST,Saito A,Azakami H,Kato A

更新日期：2003-02-01 00:00:00

abstract：:Fusion proteins expressed in bacteria are often insoluble or inefficiently purified by standard procedures previously reported to work well for the non-fused proteins. We report here a simple but general procedure that can be used to quickly customize and optimize the purification of milligram quantities of most GST f...

journal_title：Protein expression and purification

authors： Mercado-Pimentel ME,Jordan NC,Aisemberg GO

更新日期：2002-11-01 00:00:00

abstract：INTRODUCTION:Glycodelin is a glycoprotein with different oligosaccharides that are responsible for its diverse biological functions in contraception and immunosuppression. Therefore, it is necessary to have access to adequate amounts of glycodelin with retained carbohydrate structure for functional studies because the ...

journal_title：Protein expression and purification

authors： Sørensen S,Myrhøj V,Nguyen TH,Aaslo P,Hansen YB

更新日期：2017-02-01 00:00:00

abstract：:One of the self-protection mechanisms in Pseudomonas syringae pv. tabaci, a pathogen of tobacco wildfire, is thought to be due to its tabtoxin-resistance gene (ttr). In this study, the ttr gene was inserted into an expression vector, pQE30, and successfully expressed in Escherichia coli M15 at high levels. The purifie...

journal_title：Protein expression and purification

authors： Liu J,Le Y,Ye B,Zhen Y,Zhu C,Shen J,Zhang R

更新日期：2002-04-01 00:00:00

abstract：:Kynurenine 3-monooxygenase (KMO) is an enzyme central to the kynurenine pathway of tryptophan metabolism. KMO has been implicated as a therapeutic target in several disease states, including Huntington's disease. Recombinant human KMO protein production is challenging due to the presence of transmembrane domains, whic...

journal_title：Protein expression and purification

authors： Wilson K,Mole DJ,Binnie M,Homer NZ,Zheng X,Yard BA,Iredale JP,Auer M,Webster SP

更新日期：2014-03-01 00:00:00

abstract：:Soybean root nodules possess a developmentally regulated acid phosphatase (ACP) that exhibits the highest specificity for purine 5'-nucleoside monophosphates. The enzyme is a glycosylated dimer of 28- and 31-kDa subunits, which appear to be products of the same gene but differ in posttranslational modifications. In or...

journal_title：Protein expression and purification

authors： Penheiter AR,Klucas RV,Sarath G

更新日期：1998-10-01 00:00:00

abstract：:Ricin A chain (RTA) mutants which had been modified by the addition of three lysine residues, three lysines and an alanine, or six histidine residues at the carboxyl terminus were expressed in Escherichia coli. The recombinant proteins were purified to homogeneity by ion-exchange chromatography on CM-Sepharose CL-6B. ...

journal_title：Protein expression and purification

authors： Simpson JC,Roberts LM,Lord JM

更新日期：1995-10-01 00:00:00

abstract：:Enteropeptidase (synonym:enterokinase, EC 3.4.21.9) is a heterodimeric serine protease of the intestinal brush border that activates trypsinogen by highly specific cleavage of the trypsinogen activation peptide following the sequence (Asp)(4)-Lys. The DNA sequence encoding the light chain (catalytic subunit) of human ...

journal_title：Protein expression and purification

authors： Gasparian ME,Ostapchenko VG,Schulga AA,Dolgikh DA,Kirpichnikov MP

更新日期：2003-09-01 00:00:00

abstract：:Glycolate oxidase is a flavin-dependent enzyme in the photorespiratory pathway in plants. Here we report the heterologous expression of glycolate oxidase in Escherichia coli and an isolation procedure which results in 4 mg pure protein per gram cell paste in only 1.5 days. This corresponds to a more than 50-fold impro...

journal_title：Protein expression and purification

authors： Stenberg K,Lindqvist Y

更新日期：1996-11-01 00:00:00

abstract：:Human GRO alpha, GRO beta, and GRO gamma are neutrophil chemoattractants structurally related to IL-8 and compete with IL-8 for binding to IL-8 receptors on neutrophils. These proteins are part of a large superfamily of chemotactic cytokines, the "chemokines," members of which share striking structural similarities. W...

journal_title：Protein expression and purification

authors： Zagorski J,DeLarco JE

更新日期：1994-08-01 00:00:00

abstract：:The P(II) proteins comprise a family of widely distributed signal transduction proteins that integrate the signals of cellular nitrogen, carbon and energy status, and then regulate, by protein-protein interaction, the activity of a variety of target proteins including enzymes, transcriptional regulators and membrane t...

journal_title：Protein expression and purification

authors： Moure VR,Razzera G,Araújo LM,Oliveira MAS,Gerhardt ECM,Müller-Santos M,Almeida F,Pedrosa FO,Valente AP,Souza EM,Huergo LF

更新日期：2012-01-01 00:00:00

abstract：:Mitochondria-mediated apoptosis (programmed cell death) involves a sophisticated signaling and regulatory network that is regulated by the Bcl-2 protein family. Members of this family have either pro- or anti-apoptotic functions. An important pro-apoptotic member of this family is the cytosolic Bax. This protein is cr...

journal_title：Protein expression and purification

authors： Dingeldein APG,Lindberg MJ,Ådén J,Zhong X,Stoll R,Gröbner G

更新日期：2019-06-01 00:00:00

abstract：:Protein convertase 1/3 is a serine endoproteinase present in the regulated secretory pathway of endocrine and neuroendocrine cells. It is responsible for the processing of numerous prohormones and proneuropeptides into their biologically active moieties, often following cleavage at pairs of basic residues. The determi...

journal_title：Protein expression and purification

authors： Rabah N,Gauthier DJ,Gauthier D,Lazure C

更新日期：2004-10-01 00:00:00

abstract：:Structural biology places a high demand on proteins both in terms of quality and quantity. Although many protein expression and purification systems have been developed, an efficient and simple system which can be easily adapted is desirable. Here, we report a new system which combines improved expression, solubility ...

journal_title：Protein expression and purification

authors： Liu H,Naismith JH

更新日期：2009-02-01 00:00:00

abstract：:Botulinum neurotoxin serotype A (BoNT/A) is an extremely potent bacterial protein toxin. The Hc fragment of BoNT/A (AHc) was shown to be non-toxic, antigenic, and capable of eliciting a protective immunity in animals challenged with homologous BoNT. In this study, we synthesized AHc gene by using T4 DNA ligase and PCR...

journal_title：Protein expression and purification

authors： Chen R,Shi J,Cai K,Tu W,Hou X,Liu H,Xiao L,Wang Q,Tang Y,Wang H

更新日期：2010-05-01 00:00:00

abstract：:An antifungal protein with a chitinase-like N-terminal sequence, designated delandin, was isolated from the rice bean. The protein exhibited a molecular weight of 28 kDa and was adsorbed on both blue Affi-Gel and SP-Toyopearl. It exerted antifungal action toward Mycosphaerella arachidicola, Botrytis cinerea, Fu- sariu...

journal_title：Protein expression and purification

authors： Ye XY,Ng TB

更新日期：2002-04-01 00:00:00

abstract：:The 42 kDa cleavage product from the carboxyl end of the Plasmodium falciparum merozoite surface protein 1 (MSP1(42)) is an important blood-stage malaria vaccine target. Several recombinant protein expression systems have been used for production of MSP1(42) including yeast (Saccharomyces cerevisiae and Pichia pastori...

journal_title：Protein expression and purification

authors： Shimp RL Jr,Martin LB,Zhang Y,Henderson BS,Duggan P,MacDonald NJ,Lebowitz J,Saul A,Narum DL

更新日期：2006-11-01 00:00:00

abstract：:The Pseudomonas sp. have been long recognized for their exogenous lipolytic activities yet the genus still contains a lot of unexplored strains. Due to the versatile metabolic machinery and their potential for adaptation to fluctuating environmental conditions Pseudomonas sp. are of great interest for biotechnological...

journal_title：Protein expression and purification

authors： Priyanka P,Kinsella G,Henehan GT,Ryan BJ

更新日期：2019-01-01 00:00:00

abstract：:The UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase from Escherichia coli, an enzyme involved in the biosynthesis of the bacterial peptidoglycan monomer unit, was overproduced and purified to homogeneity on a large scale, yielding 4 mg of protein per liter of bacterial culture. Crystals of the complex with the subst...

journal_title：Protein expression and purification

authors： Auger G,Martin L,Bertrand J,Ferrari P,Fanchon E,Vaganay S,Pétillot Y,van Heijenoort J,Blanot D,Dideberg O

更新日期：1998-06-01 00:00:00

abstract：:The kinetic locking-on strategy improves the selectivity of protein purification procedures based on immobilized cofactor derivatives through use of enzyme-specific substrate analogues in irrigants to promote biospecific adsorption. This paper describes the development and application of this strategy to the one-chrom...

journal_title：Protein expression and purification

authors： O'flaherty M,McMahon M,Mulcahy P

更新日期：1999-02-01 00:00:00

abstract：:Pullulanase is crucial to the specific hydrolysis of branch points in amylopectin and is generally employed as an important enzyme in the starch-processing industry. Recombinant Bacillus subtilis that employs an inducible promoter would be a suitable candidate for pullulanase expression because of its safety and contr...

journal_title：Protein expression and purification

authors： Deng Y,Nie Y,Zhang Y,Wang Y,Xu Y

更新日期：2018-08-01 00:00:00

abstract：:The SaeRS two-component system in Staphylococcus aureus controls the expression of a series of virulence factors, such as hemolysins, proteases, and coagulase. The response regulator, SaeR, belongs to the OmpR family with an N-terminal regulatory domain and a C-terminal DNA binding domain. To improve the production an...

journal_title：Protein expression and purification

authors： Fan R,Shi X,Guo B,Zhao J,Liu J,Quan C,Dong Y,Fan S

更新日期：2021-01-01 00:00:00

abstract：:The B-subunits of replicative DNA polymerases belong to the superfamily of calcineurin-like phosphoesterases and are conserved from Archaea to humans. Recently we and others have shown that the B-subunit (DP1) of the archaeal family D DNA polymerase is responsible for proofreading 3'-5' exonuclease activity. The simil...

journal_title：Protein expression and purification

authors： Jokela M,Raki M,Heikkinen K,Sepponen K,Eskelinen A,Syväoja JE

更新日期：2005-09-01 00:00:00

abstract：:Human Relaxin 2 is an insulin-related peptide hormone with a mass of 19,084 Da. The mRNA contains a number of arginine codons that are rarely used by Escherichia coli to produce highly expressed proteins. As a result, expressing this recombinant protein in E. coli is problematic. When human Relaxin 2 was expressed in ...

journal_title：Protein expression and purification

authors： Kerrigan JJ,McNulty DE,Burns M,Allen KE,Tang X,Lu Q,Trulli JM,Johanson KO,Kane JF

更新日期：2008-08-01 00:00:00

abstract：:Huperzine A (HupA) is a drug used for the treatment of Alzheimer's disease. However, the biosynthesis of this medicinally important compound is not well understood. The HupA biosynthetic pathway is thought to be initiated by the decarboxylation of lysine to form cadaverine, which is then converted to 5-aminopentanal b...

journal_title：Protein expression and purification

authors： Yang H,Peng S,Zhang Z,Yan R,Wang Y,Zhan J,Zhu D

更新日期：2016-12-01 00:00:00

abstract：:Two DNA polymerase genes have been isolated from Thermococcus strains, Thermococcus zilligii from New Zealand, and the other, Thermococcus 'GT', a fast-growing strain isolated from the Galapagos trench. Both genes were isolated by genomic walking PCR, a technique that does not require expression of the gene product. P...

journal_title：Protein expression and purification

authors： Griffiths K,Nayak S,Park K,Mandelman D,Modrell B,Lee J,Ng B,Gibbs MD,Bergquist PL

更新日期：2007-03-01 00:00:00

abstract：:Streptococcal protein G has been used extensively for the purification of antibodies using the interaction of the Fc region with protein G. Many antibodies also interact with protein G through a low-affinity binding site for the Fab region. The exploitation of this low-affinity interaction for the purification of Fab'...

journal_title：Protein expression and purification

authors： Proudfoot KA,Torrance C,Lawson AD,King DJ

更新日期：1992-10-01 00:00:00

abstract：:Deoxynivalenol (DON), a mycotoxin produced by several Fusarium species, is a worldwide contaminant of food and feedstuffs. The DON-specific single-chain variable fragment (scFv) antibody was produced in recombinant Escherichia coli. The variable regions of the heavy chain (V(H)) and light chain (V(L)) cloned from the ...

journal_title：Protein expression and purification

authors： Choi GH,Lee DH,Min WK,Cho YJ,Kweon DH,Son DH,Park K,Seo JH

更新日期：2004-05-01 00:00:00

abstract：:The TCL1 gene, which is located on chromosome 14, plays a major role in human hematopoietic malignancies and encodes a 14-kDa protein whose function has not been determined. This gene is expressed in pre-B cells, in immature thymocytes, and, at low levels, in activated T cells but not in peripheral mature B cells and ...

journal_title：Protein expression and purification

authors： Du Bois GC,Song SP,Kulikovskaya I,Rothstein JL,Germann MW,Croce CM

更新日期：2000-04-01 00:00:00

abstract：:Hydrophobic charge-induction chromatography (HCIC) using 4-mercaptoethylpyridine (4-MEP) as the ligand is used to purify antibodies. The 4-MEP resin ligand has high affinity for antibodies, which makes it difficult to optimize the elution conditions. Recent studies showed that arginine is effective at eluting and puri...

journal_title：Protein expression and purification

authors： Hirano A,Maruyama T,Shiraki K,Arakawa T,Kameda T

更新日期：2017-01-01 00:00:00