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Bax to the future - A novel, high-yielding approach for purification and expression of full-length Bax protein for structural studies.

Abstract：

:Mitochondria-mediated apoptosis (programmed cell death) involves a sophisticated signaling and regulatory network that is regulated by the Bcl-2 protein family. Members of this family have either pro- or anti-apoptotic functions. An important pro-apoptotic member of this family is the cytosolic Bax. This protein is crucial for the onset of apoptosis by perforating the mitochondrial outer membrane (MOM). This process can be seen as point of no return, since disintegration of the MOM leads to the release of apotogenic factors such as cytochrome c into the cytosol triggering the activation of caspases and subsequent apoptotic steps. Bax is able to interact with the MOM with both its termini, making it inherently difficult to express in E. coli. In this study, we present a novel approach to express and purify full-length Bax with significantly increased yields, when compared to the commonly applied strategy. Using a double fusion approach with an N-terminal GST-tag and a C-terminal Intein-CBD-tag, we were able to render both Bax termini inactive and prevent disruptive interactions from occurring during gene expression. By deploying an Intein-CBD-tag at the C-terminus we were further able to avoid the introduction of any artificial residues, hence ensuring the native like activity of the membrane-penetrating C-terminus of Bax. Further, by engineering a His6-tag to the C-terminus of the CBD-tag we greatly improved the robustness of the purification procedure. We report yields for pure, full-length Bax protein that are increased by an order of magnitude, when compared to commonly used Bax expression protocols.

journal_name

Protein Expr Purif

journal_title

Protein expression and purification

authors

Dingeldein APG,Lindberg MJ,Ådén J,Zhong X,Stoll R,Gröbner G

doi

10.1016/j.pep.2019.02.004

subject

Has Abstract

pub_date

2019-06-01 00:00:00

pages

20-26

eissn

1046-5928

issn

1096-0279

pii

S1046-5928(18)30226-2

journal_volume

158

pub_type

杂志文章

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