Human CYP1A1 allelic variants: baculovirus expression and purification, hydrodynamic, spectral, and catalytical properties and their potency in the formation of all-trans-retinoic acid.

abstract：:The gene encoding a novel glucoamylase (GlucaM) from the Corallococcus sp. strain EGB was cloned and heterologous expressed in Escherichia coli BL21(DE3), and the enzymatic characterization of recombinant GlucaM (rGlucaM) was determined in the study. The glucaM had an open reading frame of 1938 bp encoding GlucaM of 6...

journal_title：Protein expression and purification

authors： Li Z,Ji K,Dong W,Ye X,Wu J,Zhou J,Wang F,Chen Q,Fu L,Li S,Huang Y,Cui Z

更新日期：2017-01-01 00:00:00

abstract：:A cDNA clone, lambda GTHP1del, encoding glutathione transferase (GST) P1-1, was isolated from a human K562 erythroleukemia cell line cDNA library. The coding sequence was lacking the codons for the N-terminal 34 amino acids. A DNA segment was designed in order to obtain the missing portion and a structure representing...

journal_title：Protein expression and purification

authors： Kolm RH,Stenberg G,Widersten M,Mannervik B

更新日期：1995-06-01 00:00:00

abstract：:A full-length hexokinase cDNA was cloned from Solanum chacoense, a wild relative of the cultivated potato. Analysis of the predicted primary sequence suggested that the protein product, ScHK2, may be targeted to the secretory pathway and inserted in the plant plasma membrane, facing the cytosol. ScHK2 was expressed as...

journal_title：Protein expression and purification

authors： Claeyssen E,Wally O,Matton DP,Morse D,Rivoal J

更新日期：2006-05-01 00:00:00

abstract：:Cardiomyopathy-related mutations in human cardiac troponin subunits, including troponin C (hcTnC), troponin I (hcTnI), and troponin T (hcTnT), are well-documented. Recently, it has been recognised that human cardiac troponin (hcTn) is a sophisticated allosteric system. Therefore, the effect of drugs on this protein co...

journal_title：Protein expression and purification

authors： Lassalle MW

更新日期：2013-02-01 00:00:00

abstract：:A baculovirus vector system that expresses cloned DNA sequences as glutathione S-transferase fusion proteins was developed. This system was used to express and purify the lymphocyte-specific tyrosine kinase p56lck. This recombinant p56lck was purified to homogeneity in a single chromatography step using glutathione re...

journal_title：Protein expression and purification

authors： Spana C,O'Rourke EC,Bolen JB,Fargnoli J

更新日期：1993-10-01 00:00:00

abstract：:Plasmids pQE-60 and pQE-30 containing 6 x His-tag sequence were used for expression of fragments of HCV structural and non-structural proteins in Escherichia coli (E. coli). The following fragments were used: core (1-98 aa), NS3 (202-482 aa), and tetramer of hypervariable region 1 (HVR1) of E2 protein. The constructed...

journal_title：Protein expression and purification

authors： Mihailova M,Fiedler M,Boos M,Petrovskis I,Sominskaya I,Roggendorf M,Viazov S,Pumpens P

更新日期：2006-11-01 00:00:00

abstract：:The aim of this study was to evaluate the parameters that affect the production of the recombinant 10 kDa culture filtrate protein (CFP10), a promising reagent of high specificity for intradermoreaction and other antigen-based methods used in the diagnosis of tuberculosis. Conditions of Escherichia coli growth tempera...

journal_title：Protein expression and purification

authors： Dela Coletta Troiano Araújo L,Wibrantz M,Rodríguez-Fernández DE,Karp SG,Talevi AC,Maltempi de Souza E,Soccol CR,Thomaz-Soccol V

更新日期：2019-02-01 00:00:00

abstract：:Failure to express soluble proteins in bacteria is mainly attributed to the properties of the target protein itself, as well as the choice of the vector, the purification tag and the linker between the tag and protein, and codon usage. The expression of proteins with fusion tags to facilitate subsequent purification s...

journal_title：Protein expression and purification

authors： Kurz M,Cowieson NP,Robin G,Hume DA,Martin JL,Kobe B,Listwan P

更新日期：2006-11-01 00:00:00

abstract：:The bivalent anti-human anti-T cell immunotoxin A-dmDT390-bisFv(G(4)S) was developed for treatment of T cell leukemia, autoimmune diseases, and tolerance induction for transplantation. The multi-domain structure of the bivalent immunotoxin hinders efficient production in Escherichia coli and most eukaryotes are sensit...

journal_title：Protein expression and purification

authors： Woo JH,Liu YY,Mathias A,Stavrou S,Wang Z,Thompson J,Neville DM Jr

更新日期：2002-07-01 00:00:00

abstract：:Adrenal cytochrome b561 (cyt b561) is the prototypical member of an emerging family of proteins that are distributed widely in vertebrate, invertebrate and plant tissues. The adrenal cytochrome is an integral membrane protein with two b-type hemes and six predicted transmembrane helices. Adrenal cyt b561 is involved i...

journal_title：Protein expression and purification

authors： Liu W,Rogge CE,Kamensky Y,Tsai AL,Kulmacz RJ

更新日期：2007-12-01 00:00:00

abstract：:Staphylococcal enterotoxins (SEs) are powerful superantigens that stimulate non-specific T-cell proliferation produced by Staphylococcus aureus and draw considerable attention as ideal drugs for cancer therapy. The filtrate of S. aureus culture has been used as ampul named Staphylococcal enterotoxin C injection in cli...

journal_title：Protein expression and purification

authors： Pan YQ,Ding D,Li DX,Chen SQ

更新日期：2007-12-01 00:00:00

abstract：:High affinity interaction between S-protein and S-peptide fragments of bovine pancreatic RNase A has been recently used for construction of molecular vehicles for targeted drug delivery. The vehicle is assembled as a complex of drug carrier conjugated S-protein with S-peptide-tagged targeting protein. To avoid random ...

journal_title：Protein expression and purification

authors： Backer MV,Gaynutdinov TI,Aloise R,Przekop K,Backer JM

更新日期：2002-12-01 00:00:00

abstract：:Vascular endothelial growth factor (VEGF) is one of the most significant mediators of angiogenesis, which interacts with a specific membrane receptor: VEGF receptor 2 (VEGFR2). Studies elsewhere have shown that, a VEGF-blocker can regulate several vital processes of tumor promotion. However, there is no literature evi...

journal_title：Protein expression and purification

authors： Cao W,Li H,Zhang J,Li D,Acheampong DO,Chen Z,Wang M

更新日期：2013-08-01 00:00:00

abstract：:The guanine nucleotide exchange factor EF-1beta gene from the thermoacidophilic archaeon Sulfolobus solfataricus (SsEF-1beta) was amplified by PCR and cloned into the pT7-7 expression vector. One of four selected clones harbored the T160C nucleotide substitution leading to the Y54H amino acid change in a hydrophobic r...

journal_title：Protein expression and purification

authors： Ianniciello G,Masullo M,Raimo G,Arcari P,Bocchini V

更新日期：1998-02-01 00:00:00

abstract：:Human interleukin-7 (IL-7) is a member of the interleukin family. Numerous studies have demonstrated IL-7's effect on B- and T-cell development as well as its potential in various clinical applications. Previously, a study reported that IL-7 could be purified from inclusion bodies using a prokaryotic system, however, ...

journal_title：Protein expression and purification

authors： Luo Y,Kong X,Xu A,Jin S,Wu D

更新日期：2009-01-01 00:00:00

abstract：:In plant, the first and the third steps of the synthesis of methionine and threonine are catalyzed by a bifunctional enzyme, aspartate kinase-homoserine dehydrogenase (AK-HSDH). In this study, we report the first purification and characterization of a highly active threonine-sensitive AK-HSDH from plants (Arabidopsis ...

journal_title：Protein expression and purification

authors： Paris S,Wessel PM,Dumas R

更新日期：2002-02-01 00:00:00

abstract：:Nedd4 is an E3 ubiquitin ligase that has received increased attention due to its role in the maintenance of proteostasis and in cellular stress responses. Investigation of Nedd4 enzymology has revealed a complex enzymatic mechanism that involves intermolecular interactions with upstream E2 conjugating enzymes and with...

journal_title：Protein expression and purification

authors： Hatstat AK,McCafferty DG

更新日期：2020-09-01 00:00:00

abstract：:Pokeweed antiviral protein (PAP)-I from the spring leaves of Phytolacca americana is a naturally occurring RNA-depurinating enzyme with broad-spectrum antiviral activity. Interest in PAP is growing due to its use as a potential anti-HIV agent. However, the clinical use of native PAP is limited due to inherent difficul...

journal_title：Protein expression and purification

authors： Rajamohan F,Doumbia SO,Engstrom CR,Pendergras SL,Maher DL,Uckun FM

更新日期：2000-03-01 00:00:00

abstract：:The recombinant truncated endolysin LysK consisting of two catalytic domains, N-terminal CHAP and amidase-2 (LysKCA) was overexpressed in E. coli in the form of inclusion bodies (IBs). These IBs were dissolved in 6 M solution of urea followed by the refolding process. The refolding efficacy of the dilution and matrix-...

journal_title：Protein expression and purification

authors： Alaksandr Ž,Sergey G,Maksim P,Sergey K,Niyaz S,Uladzimir P,Mikhail S

更新日期：2020-10-01 00:00:00

abstract：:Production of antibody fragments in heterologous hosts such as Escherichiacoli provides a unique and cost-effective method to develop engineered vectors for tumor targeting. A single-chain Fragment variable (scFv) of the murine monoclonal antibody MAb-B43.13 targeting CA125 in epithelial ovarian cancer was previously ...

journal_title：Protein expression and purification

authors： Sharma SK,Suresh MR,Wuest FR

更新日期：2014-10-01 00:00:00

abstract：:Peptide-based affinity tags are commonly used in recombinant production/purification of proteins, and are often preceded or followed by a protease recognition sequence to allow tag removal. We describe a rat monoclonal antibody 2H5 recognizing an undecapeptide tag called "eTev", which contains a recognition sequence f...

journal_title：Protein expression and purification

authors： Tabata S,Kitago Y,Fujii Y,Mihara E,Tamura-Kawakami K,Norioka N,Takahashi K,Kaneko MK,Kato Y,Takagi J

更新日期：2018-07-01 00:00:00

abstract：:Soluble guanylate cyclase (sGC) has been purified from 100 L cell culture infected by baculovirus using the newer and highly effective titerless infected-cells preservation and scale-up (TIPS) method. Successive passage of the enzyme through DEAE, Ni(2+)-NTA, and POROS Q columns obtained approximately 100mg of protein...

journal_title：Protein expression and purification

authors： Emmons TL,Mathis KJ,Shuck ME,Reitz BA,Curran DF,Walker MC,Leone JW,Day JE,Bienkowski MJ,Fischer HD,Tomasselli AG

更新日期：2009-06-01 00:00:00

abstract：:Receptor for activated C-kinase (RACK1) binds to protein kinase C and functions as an anchor for several other cellular components. Most in vitro studies of RACK1 have been carried out with RACK1 fused to a soluble fusion protein partner, such as GST or MBP. Here, we show that fusion complexes may exist as large solub...

journal_title：Protein expression and purification

authors： Bjørndal B,Trave G,Hageberg I,Lillehaug JR,Raae AJ

更新日期：2003-09-01 00:00:00

abstract：:Homocysteine alpha,gamma-lyase from the anaerobic protozoan parasite Trichomonas vaginalis has been cloned from genomic DNA using PCR methods and expressed in Escherichia coli with a vector containing the T7 promoter. The recombinant homocysteine alpha,gamma-lyase (rHCYase) is expressed as the major protein in the hos...

journal_title：Protein expression and purification

authors： Han Q,Lenz M,Tan Y,Xu M,Sun X,Tan X,Tan X,Tang L,Miljkovic D,Hoffman RM

更新日期：1998-11-01 00:00:00

abstract：:Fusion proteins expressed in bacteria are often insoluble or inefficiently purified by standard procedures previously reported to work well for the non-fused proteins. We report here a simple but general procedure that can be used to quickly customize and optimize the purification of milligram quantities of most GST f...

journal_title：Protein expression and purification

authors： Mercado-Pimentel ME,Jordan NC,Aisemberg GO

更新日期：2002-11-01 00:00:00

abstract：:Antimicrobial peptide LL-37 plays an important role in human body's first line of defense against infection. To better understand the mechanism of action, it is critical to elucidate the three-dimensional structure of LL-37 in complex with bacterial membranes. We present a bacterial expression system that allows the i...

journal_title：Protein expression and purification

authors： Li Y,Li X,Wang G

更新日期：2006-06-01 00:00:00

abstract：:Folding and assembly studies with alpha-helical membrane proteins are often hampered by the absence of high-level expression systems as well as by missing suitable in vitro refolding procedures. Experimental constraints and requirements for heterologous expression and in vitro assembly of cytochrome b6 have been exami...

journal_title：Protein expression and purification

authors： Prodöhl A,Dreher C,Hielscher R,Hellwig P,Schneider D

更新日期：2007-12-01 00:00:00

abstract：:Two DNA polymerase genes have been isolated from Thermococcus strains, Thermococcus zilligii from New Zealand, and the other, Thermococcus 'GT', a fast-growing strain isolated from the Galapagos trench. Both genes were isolated by genomic walking PCR, a technique that does not require expression of the gene product. P...

journal_title：Protein expression and purification

authors： Griffiths K,Nayak S,Park K,Mandelman D,Modrell B,Lee J,Ng B,Gibbs MD,Bergquist PL

更新日期：2007-03-01 00:00:00

abstract：:3-Deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS) [EC 4.1.2.16] is the first and rate-limiting enzyme in the 3-deoxy-d-manno-octulosonate (KDO) biosynthetic pathway. The enzyme is widely expressed in bacteria and plants. Their well conserved protein sequences imply a similar oligomeric arrangement. However, t...

journal_title：Protein expression and purification

authors： Zhang F,Xu Y,Zhang Z

更新日期：2014-09-01 00:00:00

abstract：:Influenza A virus displays one of the highest infection rates of all human viruses and therefore represents a severe human health threat associated with an important economical challenge. Influenza matrix protein 2 (M2) is a membrane protein of the viral envelope that forms a proton selective ion channel. Here we repo...

journal_title：Protein expression and purification

authors： Desuzinges Mandon E,Traversier A,Champagne A,Benier L,Audebert S,Balme S,Dejean E,Rosa Calatrava M,Jawhari A

更新日期：2017-03-01 00:00:00