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Analysis of the tyrosine protein kinase p56lck expressed as a glutathione S-transferase fusion protein in Spodoptera frugiperda cells.

Abstract：

:A baculovirus vector system that expresses cloned DNA sequences as glutathione S-transferase fusion proteins was developed. This system was used to express and purify the lymphocyte-specific tyrosine kinase p56lck. This recombinant p56lck was purified to homogeneity in a single chromatography step using glutathione resin. By SDS gel analysis recombinant p56lck was found to migrate as two species with molecular masses of approximately 56,000 Da. p56lck purified in this manner retained a high level of activity, phosphorylated an exogenous substrate on tyrosine residues, and underwent autophosphorylation on tyrosine residues. The Km (approximately 0.33 mmol) and Vmax (approximately 83 pmol min-1 mg-1) values were also determined by using enolase as a substrate.

journal_name

Protein Expr Purif

journal_title

Protein expression and purification

authors

Spana C,O'Rourke EC,Bolen JB,Fargnoli J

doi

10.1006/prep.1993.1051

subject

Has Abstract

pub_date

1993-10-01 00:00:00

pages

390-7

issue

5

eissn

1046-5928

issn

1096-0279

pii

S104659288371051X

journal_volume

4

pub_type

杂志文章

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