Systematic optimization of expression and refolding of the Plasmodium falciparum cysteine protease falcipain-2.

abstract：:Insulin-like growth factor binding proteins (IGFBPs) display many functions in humans including regulation of the insulin-like growth factor (IGF) signaling pathway. The various roles of human IGFBPs make them attractive protein candidates in drug discovery. Structural and functional knowledge on human proteins with t...

journal_title：Protein expression and purification

authors： Wanscher ASM,Williamson M,Ebersole TW,Streicher W,Wikström M,Cazzamali G

更新日期：2015-04-01 00:00:00

abstract：:Recombinant protein expression in insect cells varies greatly from protein to protein. A fusion tag that is not only a tool for detection and purification, but also enhances expression and/or solubility would greatly facilitate both structure/function studies and therapeutic protein production. We have shown that fusi...

journal_title：Protein expression and purification

authors： Liu L,Spurrier J,Butt TR,Strickler JE

更新日期：2008-11-01 00:00:00

abstract：:We have cloned ansB (YPTB1411) gene from Yersinia pseudotuberculosis Q66CJ2 and constructed stable inducible expression system that overproduce L-asparaginase from Y. pseudotuberculosis (YpA) in Escherichiacoli BL21 (DE3) cells. For purification of YpA we used Q-Sepharose and DEAE-Toyopearl column chromatography. We e...

journal_title：Protein expression and purification

authors： Pokrovskaya MV,Aleksandrova SS,Pokrovsky VS,Omeljanjuk NM,Borisova AA,Anisimova NY,Sokolov NN

更新日期：2012-03-01 00:00:00

abstract：:Acetyl-CoA C-acetyltransferase synthase gene (AACT) cDNA, DNA and promoter were cloned from Sanghuangporus baumii. The gene ORF (1260 bp) encoded 419 amino acids. The AACT DNA includes five exons (1-84 bp, 140-513 bp, 570-1027 bp, 1090-1282 bp, 1344-1494 bp) and four introns (85-139 bp, 514-569 bp, 1028-1089 bp, 1283-...

journal_title：Protein expression and purification

authors： Wang X,Wang S,Xu X,Sun J,Ma Y,Liu Z,Sun T,Zou L

更新日期：2020-06-01 00:00:00

abstract：:Hepatitis C virus encodes two enveloped glycoproteins, E1 and E2, which are involved in viral attachment and entry into target cells. We have obtained in insect cells infected by recombinant baculovirus a chimeric secreted recombinant protein, E1(341)E2(661,) containing the ectodomains of E1 and E2. The described proc...

journal_title：Protein expression and purification

authors： Tello D,Rodríguez-Rodríguez M,Yélamos B,Gómez-Gutiérrez J,Ortega S,Pacheco B,Peterson DL,Gavilanes F

更新日期：2010-06-01 00:00:00

abstract：:The XRCC1 DNA repair protein contains two regions of approximately 100 amino acids each that share homology with the BRCT (BRCA1 carboxyl terminus) domain superfamily. These two regions of XRCC1 have been shown to interact independently with DNA ligase III and poly(ADP-ribose)polymerase as part of a mechanism involved...

journal_title：Protein expression and purification

authors： Thornton K,Forstner M,Shen MR,West MG,Rupp B,Thelen MP

更新日期：1999-07-01 00:00:00

abstract：:Post-translational modification plays crucial roles in signal transduction in eukaryotic cells. To elucidate the biological function of a protein with a specific post-translational modification, it is necessary to isolate the modified protein. However, it is difficult to incorporate a modified amino acid into a specif...

journal_title：Protein expression and purification

authors： Sugase K,Landes MA,Wright PE,Martinez-Yamout M

更新日期：2008-02-01 00:00:00

abstract：:An alpha-amylase has been purified from the thermophilic fungus Scytalidium thermophilum. A ninefold purification was achieved in a single step using fluidized bed chromatography wherein alginate was used as the affinity matrix. There are at least two isoenzymes as shown by concanavalin A (Con A)-agarose column chroma...

journal_title：Protein expression and purification

authors： Roy I,Sastry MS,Johri BN,Gupta MN

更新日期：2000-11-01 00:00:00

abstract：:Recombinant protein expression from lac derived promoters by the autoinduction regime is based on diauxic growth of Escherichia coli on glucose and lactose. Glycerol is used as a supporting carbon source during the lactose-induced expression. While this glycerol-based formulation usually provides high cell densities, ...

journal_title：Protein expression and purification

authors： Ukkonen K,Mayer S,Vasala A,Neubauer P

更新日期：2013-10-01 00:00:00

abstract：:We have investigated the suitability of Pichia pastoris as an expression system for the candidate therapeutic protein, Sonic hedgehog fused to an immunoglobulin Fc domain (Shh-Fc). Sonic hedgehog is a morphogen protein involved in the patterning of a wide range of tissues during animal embryogenesis. The presence of S...

journal_title：Protein expression and purification

authors： Shapiro RI,Wen D,Levesque M,Hronowski X,Gill A,Garber EA,Galdes A,Strauch KL,Taylor FR

更新日期：2003-06-01 00:00:00

abstract：:BetaB2-crystallin, the major subunit of beta-crystallins, is difficult to purify either from lens homogenate or from betaH-or betaL-crystallins. It has been prepared by heterologous expression in Escherichia coli. Most often, the methods used for purifying a recombinant globular protein employ the combination of ion-e...

journal_title：Protein expression and purification

authors： Jobby MK,Sharma Y

更新日期：2003-03-01 00:00:00

abstract：:This article reviews the development of immobilized-metal affinity chromatography (IMAC) and describes its most important applications. We provide an overview on the use of IMAC in protein fractionation and proteomics, in protein immobilization and detection, and on some special applications such as purification of im...

journal_title：Protein expression and purification

authors： Block H,Maertens B,Spriestersbach A,Brinker N,Kubicek J,Fabis R,Labahn J,Schäfer F

更新日期：2011-09-03 00:00:00

abstract：:Amidase signature (AS) family amidases are known to exhibit broad substrate specificity. According to the available genome sequence data, a novel AS family amidase, Pl-Ami, was identified and cloned from the genome of Parvibaculum lavamentivorans ZJB14001. The recombinant amidase was overexpressed in Escherichia coli ...

journal_title：Protein expression and purification

authors： Wu ZM,Zheng RC,Zheng YG

更新日期：2017-01-01 00:00:00

abstract：:Production of antibody fragments in heterologous hosts such as Escherichiacoli provides a unique and cost-effective method to develop engineered vectors for tumor targeting. A single-chain Fragment variable (scFv) of the murine monoclonal antibody MAb-B43.13 targeting CA125 in epithelial ovarian cancer was previously ...

journal_title：Protein expression and purification

authors： Sharma SK,Suresh MR,Wuest FR

更新日期：2014-10-01 00:00:00

abstract：:Antimicrobial peptide LL-37 plays an important role in human body's first line of defense against infection. To better understand the mechanism of action, it is critical to elucidate the three-dimensional structure of LL-37 in complex with bacterial membranes. We present a bacterial expression system that allows the i...

journal_title：Protein expression and purification

authors： Li Y,Li X,Wang G

更新日期：2006-06-01 00:00:00

abstract：:Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit epsilon N-methyltransferase (Protein methylase III, Rubisco LSMT, EC 2.1.1.43) catalyzes methylation of the epsilon-amino group of Lys-14 in the large subunit of Rubisco. In this paper, an affinity purification procedure for pea (Pisum sativum L. cv Laxton'...

journal_title：Protein expression and purification

authors： Wang P,Royer M,Houtz RL

更新日期：1995-08-01 00:00:00

abstract：:We have recently identified a new family of multidomain oxidoreductase (redox) enzymes, the MICALs, that directly regulate the actin cytoskeletal elements necessary for the morphology, motility, and trajectory of cells. Our genetic assays reveal that Mical is both necessary and sufficient for actin organization and ce...

journal_title：Protein expression and purification

authors： Wu H,Hung RJ,Terman JR

更新日期：2016-11-01 00:00:00

abstract：:Escherichia coli cells were transformed with an expression vector constructed by inserting a DNA fragment encoding a Kazal-type trypsin inhibitor from mouse seminal vesicle into pGEX-2. The cloned cells were able to produce a high yield of a chimeric polypeptide made by fusing the trypsin inhibitor to glutathione S-tr...

journal_title：Protein expression and purification

authors： Lai ML,Li SH,Chen YH

更新日期：1994-02-01 00:00:00

abstract：:Deformylation of the initiator N-formylmethionine does not always proceed to completion for proteins overexpressed in Escherichia coli. To overcome this limitation, the def gene encoding the Escherichia coli peptide deformylase was cloned into the plysS plasmid under the tetracycline (Tc) promoter control. The efficie...

journal_title：Protein expression and purification

authors： Tang J,Hernández G,LeMaster DM

更新日期：2004-07-01 00:00:00

abstract：:A recombinant Hc fragment of botulinum neurotoxin, serotype B (rBoNTB(Hc)), has been successfully expressed in a Mut+ strain of the methylotrophic yeast Pichia pastoris for use as an antigen in a proposed human vaccine. The fermentation process consisted of batch phase on glycerol, followed by glycerol and methanol fe...

journal_title：Protein expression and purification

authors： Potter KJ,Bevins MA,Vassilieva EV,Chiruvolu VR,Smith T,Smith LA,Meagher MM

更新日期：1998-08-01 00:00:00

abstract：:Mitochondria-mediated apoptosis (programmed cell death) involves a sophisticated signaling and regulatory network that is regulated by the Bcl-2 protein family. Members of this family have either pro- or anti-apoptotic functions. An important pro-apoptotic member of this family is the cytosolic Bax. This protein is cr...

journal_title：Protein expression and purification

authors： Dingeldein APG,Lindberg MJ,Ådén J,Zhong X,Stoll R,Gröbner G

更新日期：2019-06-01 00:00:00

abstract：:Wild-type and deglycosylated forms of human prostate-specific antigen were expressed in Chinese hamster ovary (CHO) cells as zymogens. ProPSA was collected from conditioned medium and purified using a single cation-exchange chromatographic step for the deglycosylated form and cation-exchange followed by gel filtration...

journal_title：Protein expression and purification

authors： Bowman KK,Clark J,Yu L,Mortara K,Radika K,Wang J,Zhan H

更新日期：2000-12-01 00:00:00

abstract：:A rabbit liver cDNA library in phage lambdagt10 was screened using the coding cDNA for human cytosolic serine hydroxymethyltransferase. A clone of 1754 bp was isolated and the nucleotide sequence showed an open reading frame of 1455 bp, which coded for rabbit cytosolic serine hydroxymethyltransferase and was flanked b...

journal_title：Protein expression and purification

authors： di Salvo ML,Delle Fratte S,De Biase D,Bossa F,Schirch V

更新日期：1998-07-01 00:00:00

abstract：:l-glutamate oxidase (GLOD), encoded by the gox gene, catalyses the transformation of l-glutamic acid into α-ketoglutaric acid (α-KG). In the present study, Pichia pastoris was used for heterologous production of GLOD following optimization of the gox coding sequence for expression in the yeast host. A series of constr...

journal_title：Protein expression and purification

authors： YaPing W,Ben R,Hong Y,Rui H,Li L,Ping'an L,Lixin M

更新日期：2017-01-01 00:00:00

abstract：:The Pseudomonas sp. have been long recognized for their exogenous lipolytic activities yet the genus still contains a lot of unexplored strains. Due to the versatile metabolic machinery and their potential for adaptation to fluctuating environmental conditions Pseudomonas sp. are of great interest for biotechnological...

journal_title：Protein expression and purification

authors： Priyanka P,Kinsella G,Henehan GT,Ryan BJ

更新日期：2019-01-01 00:00:00

abstract：:The full-length cDNA that encodes the hemolytic toxin Avt-I, with 226 amino acids, from the venomous sea anemone Actineria villosa has been cloned using the oligo-capping method. The cDNA contains 681bp open reading frame and its predicted amino acid sequences revealed that Avt-I was basic polypeptides without cystein...

journal_title：Protein expression and purification

authors： Uechi G,Toma H,Arakawa T,Sato Y

更新日期：2005-04-01 00:00:00

abstract：:Human lysosomal beta-hexosaminidase exists in two major forms: the A isoform is composed of both alpha and beta chains, while the B form is a homopolymer of beta chains. Deficiency of beta-hexosaminidase underlies the GM2 gangliosidoses. We have produced active beta-hexosaminidase B in cultured insect (Sf9) cells by i...

journal_title：Protein expression and purification

authors： Boose JA,Tifft CJ,Proia RL,Myerowitz R

更新日期：1990-11-01 00:00:00

abstract：:Pseudomonas fluorescens is a robust protein expression system that is very well suited for high throughput protein expression for structural genomics studies. Since NMR spectroscopy and X-ray crystallography are both used by various investigators in structure elucidation studies, the availability of target proteins la...

journal_title：Protein expression and purification

authors： Madduri K,Badger M,Li ZS,Xu X,Thornburgh S,Evans S,Dhadialla TS

更新日期：2009-05-01 00:00:00

abstract：:Recombinant OSU VP4 protein, and outer capsid antigen of porcine rotavirus, was purified to a high level from the spent broth of baculovirus-infected Spodoptera frugiperda insect cells. Initial clarification of the broth with a 0-60% ammonium sulfate cut retained 93% of the total VP4. Q-sepharose ion exchange chromato...

journal_title：Protein expression and purification

authors： Juarbe-Osorio LG,Gorziglia M,Betenbaugh MJ

更新日期：1993-06-01 00:00:00

abstract：:Quinolinate synthetase catalyzes the second step of the de novo biosynthetic pathway of pyridine nucleotide formation. In particular, quinolinate synthetase is involved in the condensation of dihydroxyacetone phosphate and iminoaspartate to form quinolinic acid. To study the mechanism of action, the specificity of the...

journal_title：Protein expression and purification

authors： Ceciliani F,Caramori T,Ronchi S,Tedeschi G,Mortarino M,Galizzi A

更新日期：2000-02-01 00:00:00