Purification and characterization of recombinant rabbit cytosolic serine hydroxymethyltransferase.

abstract：:Pseudolysin is the extracellular elastase of Pseudomonas aeruginosa and belongs to the thermolysin-like family of metallopeptidases. Pseudolysin has been identified as a robust drug target and a biotechnologically important enzyme in the tanning industry. Previous attempts to purify active pseudolysin from P. aerugino...

journal_title：Protein expression and purification

authors： Odunuga OO,Adekoya OA,Sylte I

更新日期：2015-09-01 00:00:00

abstract：:Hsp47 is regarded as a collagen-specific chaperone with several suggested roles in collagen biosynthesis under normal and disease conditions. We describe here a procedure for the expression and purification of Hsp47 in Escherichia coli using the IMPACT expression system (New England Biolabs) where the guest gene is fu...

journal_title：Protein expression and purification

authors： Thomson CA,Ananthanarayanan VS

更新日期：2001-10-01 00:00:00

abstract：:Rhizopus oryzae lipase (ROL) is an important industrial enzyme limited in application due to its low production in native strains. Here, we used a new combined strategy to overexpress ROL in Pichia pastoris. An efficient method based on bio-brick was developed to construct a series of vectors harboring different copy ...

journal_title：Protein expression and purification

authors： Jiao L,Zhou Q,Su Z,Xu L,Yan Y

更新日期：2018-07-01 00:00:00

abstract：:We have investigated the suitability of Pichia pastoris as an expression system for the candidate therapeutic protein, Sonic hedgehog fused to an immunoglobulin Fc domain (Shh-Fc). Sonic hedgehog is a morphogen protein involved in the patterning of a wide range of tissues during animal embryogenesis. The presence of S...

journal_title：Protein expression and purification

authors： Shapiro RI,Wen D,Levesque M,Hronowski X,Gill A,Garber EA,Galdes A,Strauch KL,Taylor FR

更新日期：2003-06-01 00:00:00

abstract：:The purpose of this work was to characterize the functions of two sesquiterpene synthase genes from moso bamboo (Phyllostachys edulis). Two novel sesquiterpene synthase genes, belonging to the Tpsa subfamily, were isolated from moso bamboo. MoTPS2 was 1641 bp in length and encoded a protein of 63 kDa, whereas MoTPS6 w...

journal_title：Protein expression and purification

authors： Chen X,Wang Y,Sun J,Wang J,Xun H,Tang F

更新日期：2016-04-01 00:00:00

abstract：:The TCL1 gene, which is located on chromosome 14, plays a major role in human hematopoietic malignancies and encodes a 14-kDa protein whose function has not been determined. This gene is expressed in pre-B cells, in immature thymocytes, and, at low levels, in activated T cells but not in peripheral mature B cells and ...

journal_title：Protein expression and purification

authors： Du Bois GC,Song SP,Kulikovskaya I,Rothstein JL,Germann MW,Croce CM

更新日期：2000-04-01 00:00:00

abstract：:Hepatocyte growth factor (HGF) is a multifunctional protein expressed in a variety of cell types and tissues. Here we describe a novel one-step method to separate and identify HGF, based on a unique interaction between HGF and Cu(II). Conditioned medium (CM) from mouse 3T3-L1 adipocytes which contains HGF or purified ...

journal_title：Protein expression and purification

authors： Rahimi N,Etchells S,Elliott B

更新日期：1996-05-01 00:00:00

abstract：:We describe a single method to purify milligram amounts of authentic wild-type and mutant HIV-1 and HIV-2 Tat proteins overexpressed in Escherichia coli. This method takes advantage of the highly basic, positively charged RNA binding domain present in both HIV-1 and HIV-2 Tat, which also facilitated purification of HI...

journal_title：Protein expression and purification

authors： Orsini MJ,García-Martínez LF,Mavankal G,Gaynor RB,Debouck CM

更新日期：1996-09-01 00:00:00

abstract：:As an insect-selective neurotoxin, scorpion long-chain BjαIT is a promising prospect for insecticidal application; however, the difficulty of obtaining natural BjαIT represents the major obstacle preventing analysis of its insecticidal activity against agricultural insect pests. Here, we screened recombinant Pichia pa...

journal_title：Protein expression and purification

authors： Li H,Xia Y

更新日期：2018-12-01 00:00:00

abstract：:The A(280)/A(260) ratio of a purified protein is frequently used as an indication of the purity of the preparation with respect to nucleic acids. We show here that for low-molecular-weight recombinant proteins purified from Escherichia coli, a low A(280)/A(260) ratio can also result from contamination with UDP-linked ...

journal_title：Protein expression and purification

authors： Ram MK,Andrade LJ,Phillips TB,van Schravendijk MR

更新日期：1999-11-01 00:00:00

abstract：:High affinity interaction between S-protein and S-peptide fragments of bovine pancreatic RNase A has been recently used for construction of molecular vehicles for targeted drug delivery. The vehicle is assembled as a complex of drug carrier conjugated S-protein with S-peptide-tagged targeting protein. To avoid random ...

journal_title：Protein expression and purification

authors： Backer MV,Gaynutdinov TI,Aloise R,Przekop K,Backer JM

更新日期：2002-12-01 00:00:00

abstract：:This paper reports the overproduction and the details of a rapid method to purify active sigmaS monomers from a T7 RNA polymerase-based protein expression system. This 2-day procedure involves solubilizing inclusion bodies in sarkosyl detergent, removal of sarkosyl by dialysis, and a single gel filtration column chrom...

journal_title：Protein expression and purification

authors： Nguyen LH,Burgess RR

更新日期：1996-08-01 00:00:00

abstract：:Borneol is a bicyclic plant monoterpene. It can be degraded by soil microorganisms through the conversion of borneol dehydrogenase (BDH) and a known camphor degradation pathway. Recombinant BDH from Pseudomonas sp. TCU-HL1 was produced in the form of inclusion body. The refolded BDH1 tends to precipitate. Insoluble re...

journal_title：Protein expression and purification

authors： Khine AA,Lu PC,Ko TP,Huang KF,Chen HP

更新日期：2020-11-01 00:00:00

abstract：:Bacteriocin, which is produced by lactic acid bacteria (LAB), has the potential to act as natural preservatives in the food industry. To develop strategies to overproduce such peptides, plantaricin NC8, a class IIb LAB bacteriocin that consists of two peptides, PLNC8α and PLNC8β, was successfully heterologously expres...

journal_title：Protein expression and purification

authors： Jiang H,Li P,Gu Q

更新日期：2016-11-01 00:00:00

abstract：:The UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase from Escherichia coli, an enzyme involved in the biosynthesis of the bacterial peptidoglycan monomer unit, was overproduced and purified to homogeneity on a large scale, yielding 4 mg of protein per liter of bacterial culture. Crystals of the complex with the subst...

journal_title：Protein expression and purification

authors： Auger G,Martin L,Bertrand J,Ferrari P,Fanchon E,Vaganay S,Pétillot Y,van Heijenoort J,Blanot D,Dideberg O

更新日期：1998-06-01 00:00:00

abstract：:We have developed a family of cloning vectors that direct expression of fusion proteins that mimic aggregated immunoglobulin (IgG) (AIG) and immune complex function with respect to their interactions with FcgammaR and that allow for the inclusion and targeting of a second protein domain to cells expressing FcgammaR. T...

journal_title：Protein expression and purification

authors： White DM,Jensen MA,Shi X,Qu Zx,Arnason BG

更新日期：2001-04-01 00:00:00

abstract：:A gene encoding a glutamate-specific endopeptidase (GSE) from Bacillus licheniformis (BL) has been cloned in Escherichia coli cells. The recombinant protein was expressed as cytoplasmic insoluble inclusion bodies. Immobilized metal affinity chromatography was employed to purify the protein, and then a 27-kDa GSE inter...

journal_title：Protein expression and purification

authors： Ye W,Liu J,Wang H,Wang J,Wang X

更新日期：2012-03-01 00:00:00

abstract：:The aim of this study was to establish a method of purifying intact complement factor H (CFH) from human plasma. CFH was isolated from human plasma by polyethylene glycol (PEG) precipitation, following three sequential chromatographic columns, which consisted of l-lysine Sepharose column, Resource Q column and Sephacr...

journal_title：Protein expression and purification

authors： Wang FM,Yu F,Zhao MH

更新日期：2013-10-01 00:00:00

abstract：:The assembly pathway of the insect cell Spodoptera frugiperda (Sf-9) was engineered to include expression of the murine chaperone immunoglobulin heavy chain binding protein (BiP) using the baculovirus vector. The impact of BiP coexpression on the production and secretion of functional and soluble recombinant immunoglo...

journal_title：Protein expression and purification

authors： Hsu TA,Eiden JJ,Bourgarel P,Meo T,Betenbaugh MJ

更新日期：1994-12-01 00:00:00

abstract：:Mannan outer chain N-glycan structures are yeast/fungal-specific typically found on secreted and cell wall glycoproteins. Mannan outer chains consist of an alpha-1,6 polymannose backbone attached to a Man(8-10)(GlcNAc)(2) core. The backbone contains branches of alpha-1,2 mannose residues, terminated with alpha-1,3 man...

journal_title：Protein expression and purification

authors： Rodionov D,Romero PA,Berghuis AM,Herscovics A

更新日期：2009-07-01 00:00:00

abstract：:One-step chromatography on a Mono S column allows the purification of high mobility group (HMG) proteins 1 and 2 under nondenaturing conditions. Chromatography of HMG1 and -2 on Mono S can be achieved with three of the most widely employed extraction techniques for chromosomal proteins, 0.35 M sodium chloride, 0.74 M ...

journal_title：Protein expression and purification

authors： Rice GA,Cole RD

更新日期：1990-09-01 00:00:00

abstract：:Human β-glucuronidase (GUS; EC 3.2.1.31) is a lysosomal enzyme that catalyzes the hydrolysis of β-d-glucuronic acid residues from the non-reducing termini of glycosaminoglycans. Impairment in GUS function leads to the metabolic disorder mucopolysaccharidosis type VII, also known as Sly syndrome. We produced GUS from a...

journal_title：Protein expression and purification

authors： Fratz-Berilla EJ,Ketcham SA,Parhiz H,Ashraf M,Madhavarao CN

更新日期：2017-12-01 00:00:00

abstract：:Vibriolysin, an extracellular protease of Vibrio proteolyticus, is synthesized as a preproenzyme. The N-terminal propeptide functions as an intramolecular chaperone and an inhibitor of the mature enzyme. Extracellular production of recombinant vibriolysin has been achieved in Bacillus subtilis, but not in Escherichia ...

journal_title：Protein expression and purification

authors： Sonoda H,Sugimura A

更新日期：2008-12-01 00:00:00

abstract：:An internal fragment (978 bp) corresponding to the dog zona pellucida glycoprotein-3 (DZP3), excluding the N-terminal signal sequence and the C-terminal transmembrane-like domain, was amplified by polymerase chain reaction from a full-length cDNA clone. The amplified SacI and PstI restricted fragment was cloned in-fra...

journal_title：Protein expression and purification

authors： Santhanam R,Panda AK,Kumar VS,Gupta SK

更新日期：1998-04-01 00:00:00

abstract：UNLABELLED:Recombinant protein production is a significant biotechnological process as it allows researchers to produce a specific protein in desired quantities. Escherichia coli (E. coli) is the most popular heterologous expression host for the production of recombinant proteins due to its advantages such as low cost,...

journal_title：Protein expression and purification

authors： Habibi N,Samian MR,Hashim SZ,Norouzi A

更新日期：2014-03-01 00:00:00

abstract：:A highly efficient cell-free translation system has been combined with suppressor tRNA technology to substitute nor-Tyr and 3-fluoro-Tyr in place of Tyr183 at the DNA polymerase active site of p66 of human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT). Supplementing the wild-type HIV-1 p51 RT subunit ...

journal_title：Protein expression and purification

authors： Klarmann GJ,Eisenhauer BM,Zhang Y,Sitaraman K,Chatterjee DK,Hecht SM,Le Grice SF

更新日期：2004-11-01 00:00:00

abstract：:Aminopeptidase B (Ap-B) is a ubiquitous enzyme and its physiological function still remains an open question. This Zn2+ -exopeptidase catalyzes the amino-terminal cleavage of basic residues of peptide or protein substrates, indicating a role in precursor processing. In addition, the enzyme exhibits a residual capacity...

journal_title：Protein expression and purification

authors： Cadel S,Gouzy-Darmon C,Petres S,Piesse C,Pham VL,Beinfeld MC,Cohen P,Foulon T

更新日期：2004-07-01 00:00:00

abstract：:PDZ (PSD-95, DiscsLarge, ZO1) domains function in nature as protein binding domains within scaffold and membrane-associated proteins. They comprise ∼90 residues and make specific, high affinity interactions with complementary C-terminal peptide sequences, with other PDZ domains, and with phospholipids. We hypothesized...

journal_title：Protein expression and purification

authors： Walkup WG 4th,Kennedy MB

更新日期：2014-06-01 00:00:00

abstract：:Rat granulocyte macrophage colony-stimulating factor (rGM-CSF) cDNA was amplified and cloned, and recombinant-rGM-CSF (R-rGM-CSF) was expressed and isolated from Escherichia coli. The synthesis of R-rGM-CSF was directed by a modified, inducible maltose binding protein (MBP) gene fusion expression vector, pMTR-23, and ...

journal_title：Protein expression and purification

authors： Holowachuk EW,Ruhoff MS

更新日期：1995-10-01 00:00:00

abstract：:Human interleukin-7 (IL-7) is a member of the interleukin family. Numerous studies have demonstrated IL-7's effect on B- and T-cell development as well as its potential in various clinical applications. Previously, a study reported that IL-7 could be purified from inclusion bodies using a prokaryotic system, however, ...

journal_title：Protein expression and purification

authors： Luo Y,Kong X,Xu A,Jin S,Wu D

更新日期：2009-01-01 00:00:00