Large-scale preparation, purification, and crystallization of UDP-N-acetylmuramoyl-L-alanine: D-glutamate ligase from Escherichia coli.

abstract：:Cardiomyopathy-related mutations in human cardiac troponin subunits, including troponin C (hcTnC), troponin I (hcTnI), and troponin T (hcTnT), are well-documented. Recently, it has been recognised that human cardiac troponin (hcTn) is a sophisticated allosteric system. Therefore, the effect of drugs on this protein co...

journal_title：Protein expression and purification

authors： Lassalle MW

更新日期：2013-02-01 00:00:00

abstract：:HtrA2 is an apoptosis-activating protein to enhance the apoptotic process by preventing the formation of the IAP-caspase complex, thus freeing caspase to trigger the apoptosis pathway. Here, we presented the full-length sequence of HtrA2 from the black tiger shrimp (PmHtrA2). The full-length PmHtrA2 transcript was 140...

journal_title：Protein expression and purification

authors： Suwannaboon R,Phiwsaiya K,Senapin S,Khunrae P,Rattanarojpong T

更新日期：2013-12-01 00:00:00

abstract：:Dehydrodolichyl diphosphate synthase (DDPPs) catalyzes the sequential condensation of isopentenyl diphosphate with farnesyl diphosphate to synthesize long-chain dehydrodolichyl diphosphate, which serves as a precursor of glycosyl carrier in glycoprotein biosynthesis in eukaryotes. To perform kinetic and structural stu...

journal_title：Protein expression and purification

authors： Chang SY,Tsai PC,Tseng CS,Liang PH

更新日期：2001-12-01 00:00:00

abstract：:Acyl coenzyme A binding protein (ACBP) has been proposed to transport fatty acyl CoAs intracellularly, facilitating their metabolism. In this study, a new mouse recombinant ACBP was produced by insertion of a histidine (his) tag at the C-terminus to allow efficient purification by Ni-affinity chromatography. The his-t...

journal_title：Protein expression and purification

authors： Petrescu AD,Huang H,Hostetler HA,Schroeder F,Kier AB

更新日期：2008-04-01 00:00:00

abstract：:Plants produce a large number of cellulases that are either secreted or anchored in the plasma membrane where they likely function in various aspects of cellulose synthesis, modification and degradation during plant growth and development. Very few of these enzymes have been characterized in any detail, however. Here ...

journal_title：Protein expression and purification

authors： Tawde MD,Freimuth P

更新日期：2012-10-01 00:00:00

abstract：:The human PRODH gene has been shown to have unique roles in regulating cell survival and apoptotic pathways and it has been related to velocardiofacial syndrome/DiGeorge syndrome and increased susceptibility to schizophrenia. It encodes for the flavoprotein proline oxidase (PO), which catalyzes the conversion of l-pro...

journal_title：Protein expression and purification

authors： Tallarita E,Pollegioni L,Servi S,Molla G

更新日期：2012-04-01 00:00:00

abstract：:The recombinant human malonyl-CoA decarboxylase (hMCD) was overexpressed in Escherichia coli with and without the first 39 N-terminal amino acids via a cleavable MBP-fusion construct. Proteolytic digestion using genenase I to remove the MBP-fusion tag was optimized for both the full length and truncated hMCD. The apo-...

journal_title：Protein expression and purification

authors： Zhou D,Yuen P,Chu D,Thon V,McConnell S,Brown S,Tsang A,Pena M,Russell A,Cheng JF,Nadzan AM,Barbosa MS,Dyck JR,Lopaschuk GD,Yang G

更新日期：2004-04-01 00:00:00

abstract：:Leptin is a 16-kDa nonglycosylated hormone that is produced in mature adipocytes and which acts primarily in the hypothalamus to reduce food intake and body weight. While the rat is a representative laboratory animal model in obesity research, so far recombinant rat leptin was not available. In the present study, rat ...

journal_title：Protein expression and purification

authors： Park JH,Lee HH,Na SY,Ju SK,Lee YJ,Lee MK,Kim KL

更新日期：2001-06-01 00:00:00

abstract：:Listeriolysin O (LLO) is a cholesterol-binding sulfhydryl-activated hemolysin encoded by Listeria monocytogenes hlyA gene. After analyzing the nucleotide coding sequence of this gene from the ATCC 9525 L. monocytogenes strain, we cloned it in a pET vector for expression in Escherichia coli. Thanks to the optimization ...

journal_title：Protein expression and purification

authors： Giammarini C,Andreoni F,Amagliani G,Casiere A,Barocci S,Magnani M

更新日期：2003-03-01 00:00:00

abstract：:Escherichia coli oligoribonuclease (EcoORN), encoded by the orn gene, is a 3'-5' exonuclease that degrades short single-stranded oligoribonucleotides to rNMPs in the final step of RNA degradation. The orn gene is essential in E. coli, but not in higher organisms, and close homologues are present in other genomes from ...

journal_title：Protein expression and purification

authors： Young Park A,Elvin CM,Hamdan SM,Wood RJ,Liyou NE,Hamwood TE,Jennings PA,Dixon NE

更新日期：2008-02-01 00:00:00

abstract：:The hexokinase (ATP;D-hexose 6-phosphotransferase, EC 2.7.1.1) of Schistosoma mansoni has been expressed in Escherichia coli as a fusion protein including an N-terminal polyhistidine tag and enterokinase cleavage site. The enzyme was purified by metal chelate chromatography to > 95% homogeneity, based on analysis by S...

journal_title：Protein expression and purification

authors： Armstrong RL,Wilson JE,Shoemaker CB

更新日期：1996-11-01 00:00:00

abstract：:The ubiquitin system represents a selective mechanism for intracellular proteolysis in eukaryotic cells that involves the sequential activity of three enzymes, ubiquitin-activating enzyme (E1), ubiquitin-conjugating enzyme (E2), and ubiquitin-protein ligase (E3). The identification of these proteins and their cellular...

journal_title：Protein expression and purification

authors： Reyes LF,Sommer CA,Beltramini LM,Henrique-Silva F

更新日期：2006-02-01 00:00:00

abstract：:Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), which is capable of selectively inducing apoptosis of cancer cells, is a potential targeted drug for cancer therapy. The TRAIL protein induces apoptosis only in trimeric form. However, the recombinant soluble TRAIL (sTRAIL) trimer has low stability and a...

journal_title：Protein expression and purification

authors： Wang B,Wang Z,Yan J,Wang L,Wang Z,Wu J,Zhang H,Wu H,Kong W,Yu B,Yu X

更新日期：2015-11-01 00:00:00

abstract：:Transcription factor HIF-1 is a key regulator in cellular adaptation to hypoxia. HIF prolyl hydroxylases (PHDs) control HIF-1 accumulation by hydroxylation dependent on molecular oxygen. Due to this regulation, PHDs have been pointed out as potential drug targets. We have purified catalytically active human PHD3 after...

journal_title：Protein expression and purification

authors： Fedulova N,Hanrieder J,Bergquist J,Emrén LO

更新日期：2007-07-01 00:00:00

abstract：:NF-kappaB is a pleiotropic transcriptional activator originally identified by its ability to regulate immunoglogulin kappa light chain expression. Purification of this DNA-binding complex demonstrated that NF-kappaB is a heterodimer composed of two subunits, NFKB1 and RelA. Previous studies have shown that truncated v...

journal_title：Protein expression and purification

authors： Coleman TA,Huddleston KA,Ruben SM,Rosen CA,Gentz R

更新日期：1997-02-01 00:00:00

abstract：:Lactobacillus β-galactosidases are mostly heterodimeric proteins, which are encoded by the two overlapping genes, lacL and lacM, and produced in recombinant prokaryotic systems for higher yield. This is the first report on the expression of a heterodimeric β-galactosidase from Lactobacillus crispatus B470 in Pichia pa...

journal_title：Protein expression and purification

authors： Nie C,Liu B,Zhang Y,Zhao G,Fan X,Ning X,Zhang W

更新日期：2013-11-01 00:00:00

abstract：:A procedure for the purification of the rat liver microsomal carnitine octanoyltransferase (COT) that catalyzes the reversible formation of medium-chain and long-chain acylcarnitines from acyl-coenzyme A is described. The K0.5 for L-carnitine is 0.6 mM and the K0.5 for both decanoyl-CoA and palmitoyl-CoA is 0.6 microM...

journal_title：Protein expression and purification

authors： Murthy MS,Bieber LL

更新日期：1992-02-01 00:00:00

abstract：:To expand the application of the streptavidin-biotin technology for reversible affinity purification of biotinylated proteins, a novel form of monomeric streptavidin was engineered and produced using Bacillus subtilis as the expression host. By changing as little as two amino acid residues (T90 and D128) to alanine, t...

journal_title：Protein expression and purification

authors： Qureshi MH,Wong SL

更新日期：2002-08-01 00:00:00

abstract：:We present a new streptavidin-binding peptide for both the purification and the detection of recombinant proteins. The peptide possesses nanomolar-affinity for streptavidin and therefore was termed Nano-tag. The Nano-tag(15) is 15 amino acids long and binds to streptavidin with a dissociation constant of 4 nM and the ...

journal_title：Protein expression and purification

authors： Lamla T,Erdmann VA

更新日期：2004-01-01 00:00:00

abstract：:In microorganisms and plants, mevalonate kinase is involved in the biosynthesis of isoprenoid derivatives, one of the largest groups of natural products. We subcloned the gene of mevalonate kinase from Methanococcus jannaschii into a bacterial expression vector pLM1 with six continuous histidine codons attached to the...

journal_title：Protein expression and purification

authors： Chu X,Liu X,Yau M,Leung YC,Li D

更新日期：2003-08-01 00:00:00

abstract：:The present report documents an improved enzyme assay method for the mammalian L-alanine:4,5-dioxovalerate transaminase which is of significant utility in work with crude tissue homogenates, cell cultures, or purified enzyme preparations. We also describe a new and rapid purification procedure for this enzyme from rat...

journal_title：Protein expression and purification

authors： Tyagi RK,Datta K

更新日期：1994-12-01 00:00:00

abstract：:The P(II) proteins comprise a family of widely distributed signal transduction proteins that integrate the signals of cellular nitrogen, carbon and energy status, and then regulate, by protein-protein interaction, the activity of a variety of target proteins including enzymes, transcriptional regulators and membrane t...

journal_title：Protein expression and purification

authors： Moure VR,Razzera G,Araújo LM,Oliveira MAS,Gerhardt ECM,Müller-Santos M,Almeida F,Pedrosa FO,Valente AP,Souza EM,Huergo LF

更新日期：2012-01-01 00:00:00

abstract：:Fab (fragment that having the antigen binding site) of a monoclonal antibody (mAb) is widely required in biopharmaceutical research and development. At Centocor, two routes of Fab production and purification were used to enable a variety of research and development efforts, particularly, crystallographic studies of an...

journal_title：Protein expression and purification

authors： Zhao Y,Gutshall L,Jiang H,Baker A,Beil E,Obmolova G,Carton J,Taudte S,Amegadzie B

更新日期：2009-10-01 00:00:00

abstract：:The H-NS protein is one of the major constituents of the nucleoid structure that has been implicated in the DNA packaging and in the global regulation of gene expression. The study of this transcriptional regulator is an effort to fight Xylella fastidiosa, a citrus pathogen responsible for a range of economically impo...

journal_title：Protein expression and purification

authors： Paula DP,Azzoni AR,Siqueira SF,Catani CF,Rosselli LK,de Souza AP

更新日期：2003-11-01 00:00:00

abstract：:Human granulocyte-macrophage colony-stimulating factor (GM-CSF), a hemopoietic growth factor, was produced and secreted from tobacco cell suspensions. The GM-CSF cDNA was carried by a binary vector under the control of the CaMV 35S promoter and the T7 terminator. In addition, a 5'-nontranslated region from the tobacco...

journal_title：Protein expression and purification

authors： James EA,Wang C,Wang Z,Reeves R,Shin JH,Magnuson NS,Lee JM

更新日期：2000-06-01 00:00:00

abstract：:Human paraoxonase (hPON3) is a high density lipoprotein-related glycoprotein with multi-enzymatic properties and antioxidant activity which is proposed to participate in the prevention of low density lipoprotein (LDL) oxidation. In this study, hPON3 gene was amplified from Human Fetal Liver Marathon-Ready cDNA and exp...

journal_title：Protein expression and purification

authors： Lu H,Zhu J,Zang Y,Ze Y,Qin J

更新日期：2006-03-01 00:00:00

abstract：:The AP-1 transcription factor is a dimeric protein complex formed primarily between Jun (c-Jun, JunB, JunD) and Fos (c-Fos, FosB, Fra-1, Fra-2) family members. These distinct AP-1 complexes are expressed in many cell types and modulate target gene expression implicated in cell proliferation, differentiation, and stres...

journal_title：Protein expression and purification

authors： Wang WM,Lee AY,Chiang CM

更新日期：2008-05-01 00:00:00

abstract：:The major immunodominant integral outer membrane protein C (OmpC) from Salmonella typhi Ty21a was overexpressed, without the signal peptide, in Escherichia coli. The protein aggregates as inclusion bodies (IBs) in the cytoplasm. OmpC from IBs was solubilized with 4 M urea and refolded. This involved rapid dilution of ...

journal_title：Protein expression and purification

authors： Kumar PD,Krishnaswamy S

更新日期：2005-03-01 00:00:00

abstract：:Lectins are a class of proteins with specific carbohydrate-binding properties found in a wide variety of plants and animals. Gramineae lectins are presumably defense-related proteins in plants that exert their effect by binding to N-acetylglucosamine. Barley lectin is a vacuolar protein synthesized with an amino-termi...

journal_title：Protein expression and purification

authors： Schroeder MR,Raikhel NV

更新日期：1992-12-01 00:00:00

abstract：:Most parasitic protozoa lack the de novo purine biosynthetic pathway and rely exclusively on the salvage pathway for their purine nucleotide requirements. Enzymes of the salvage pathway are, therefore, candidate drug targets. We have cloned the Plasmodium falciparum adenylosuccinate synthetase gene. In the parasite, a...

journal_title：Protein expression and purification

authors： Jayalakshmi R,Sumathy K,Balaram H

更新日期：2002-06-01 00:00:00